STUDIES ON THE MECHANISM OF LIGHT DEPENDENT CHANGES IN FLOWERCOLOR

花色光变机制的研究

• 批准号: 10460013
• 负责人: KAWABATA Saneyuki
• 金额: $ 6.91万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10460013/
• 关键词: Anthocyanin; Light; Chalcone Synthase; Chalcone Isomerase; Dihydrflavonol Reductase; Photoreceptor; 光依存性; DFR; CHI; イチゴ; カブ

To stabilize the expression of anthocyanin under changing environment, analysis of their mechanism from various point of view is required.1. There are two suggested mechanism of light dependent pigmentation: 1 ) the reaction mediated by photoreceptors, 2) those mediated by sugar supply from leaves or stems. To understand which mechanism is more important, the effects of partial shading of flowers or whole shading of plants were investigated in oriental lily 'Acapulco' and stock 'pigmy rose'. Partial shading of flowers suppressed pigmentation in both species, indicating photoreceptor mediated reaction. In lilies, whole plant shading restricted pigmentation more severely than did flower shading, indicating sugar mediated reaction.2. The expression of PAL, CHS, CHI, DFR genes were investigated in 'Nyoho' strawberry fruits as fruit development. The pigmentation was correlated to the expression of DFR and CHI. The result suggested that these two enzymes are involved in the developmental reg … More ulation of anthocyanin biosynthesis.3. The effect of light on the expression of anthocyanin biosynthesis genes were investigated in 'Toyonoka' strawberry fruits, in which anthocyanin accumulation was sensitive to light intensity. The expressions of PAL, CHS, and DFR were not influenced by light irradiation, suggesting that none of these genes are not involved in light dependent regulation of anthocyanin biosynthesis.4. The anthocyanin accumulation in 'Tsudakabu' turnip root was completely prevented when whole roots were shaded by aluminum foil. When shaded roots were irradiated, the anthocyanin started to accumulate after 20 hours and completed after 80 hours. Using 'Tsudakabu' and 'Yurugi Akamarukabu', in which the pigmentation was light-dependent and -independent, respectively, crossing experiment was conducted. Although segregation pattern of phenotypes was very complex, light dependent trait tended to be dominant. The results suggested that more than 2 genes are involved in the determination of light dependency of anthocyanin accumulation. Less

为了在变化的环境下稳定花青素的表达，需要从多个角度分析其机制。光依赖性色素沉着的机制有两种：1)光感受器介导的反应，2)叶或茎的糖供应介导的反应。本文以东方百合阿卡普尔科（Acapulco）和矮玫瑰（pigmy rose）为研究对象，研究了花的部分遮荫和植物的整体遮荫对植物生长的影响。花的部分遮荫抑制了两种植物的色素沉着，表明光感受器介导的反应。在百合花中，整株遮荫比花遮荫更严重地限制了色素沉着，表明糖介导的反应。研究了‘Nyoho’草莓果实发育过程中PAL、CHS、CHI、DFR基因的表达情况。色素沉着与DFR和CHI的表达相关。结果表明，这两种酶参与了花青素生物合成的发育过程。以“丰冈”草莓果实为材料，研究了光照对花青素合成基因表达的影响。PAL、CHS和DFR的表达不受光照影响，说明这些基因都参与了花青素生物合成的光依赖性调控。全根铝箔遮荫完全阻止了津田萝卜根部花青素的积累。遮荫根辐照后，花青素在20小时后开始积累，80小时后完全积累。以色素沉着光依赖性和非光依赖性的‘津竹’和‘玉木赤色’为材料，进行杂交试验。虽然表型分离模式非常复杂，但光依赖性状倾向于占主导地位。结果表明，花青素积累的光依赖性有2个以上基因参与。少

项目成果

Y.Li,R.Sakiyama,H.Maruyama,S.Kawabata: "Regulation of anthocyanin biosynthesis during fruit development in 'Nyoho' strawberry."J.Japan.Soc.Hort.Sci. 70(1). 28-32 (2001)

Y.Li，R.Sakiyama，H.Maruyama，S.Kawabata：““Nyoho”草莓果实发育过程中花青素生物合成的调节。”J.Japan.Soc.Hort.Sci。

Kawabata, S. et al.: "Role of photoreceptor-and sugar-mediated reactions in dependent anthocyanin production in lily and stock flower"J. Japan. Soc. Hort. Soc.. 72(印刷中). (2002)

Kawabata，S. 等人：“百合和母花中光感受器和糖介导的反应”J. Soc. Soc. 72（出版中）。

Kawabata, S. et al.: "Role of photoreceptor- and sugar-mediated reactions in light dependent anthocyanin production in lily and stock flowers."J. Japan. Soc. Hort. Soc.. (In Print).

Kawabata, S. 等人：“光感受器和糖介导的反应在百合和母花光依赖性花青素生产中的作用。”J.

Li, Y. et al.: "Regulation of anthocyanin biosynthesis during fruit deve in 'Nyoho' strawberry"J. Japan. Soc. Hort. Soc.. 70. 28-32 (2001)

Li，Y.等：“‘Nyoho’草莓果实发育过程中花青素生物合成的调控”J.

Li. Y. et al.: "Regulation of anthocyanin biosynthesis during fruit development in 'Nyoho' strawberry."J. Japan. Soc. Hort. Soc.. 70. 28-32 (2001)

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