Analysis of the dominant double-flower gene using high density RAD markers in Eustoma grandiflorum

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Linkage mapping of dominant double-flower trait was conducted in Eustoma grandiflorum. In the F2 population constructed between double-flower and single-flower accessions, double-flower with more than two whorls and flowers with abnormal floral organs were observed. The segregation ratio suggested that more than two genes were involved in the formation of double flowers. A total of 800k RAD tags was determined and 100k SNPs were found in these RAD tags. Linkage analysis was performed using these RAD markers and F2 population.