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Detection of intra-snacific variations and analysis of nonulation structure in spider mites, using genetic markers

使用遗传标记检测叶螨的内部变异并分析非结构结构

基本信息

批准号：
10460021
负责人：
TAKAFUJI Akio
金额：
$ 3.78万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

Two species of spider mites, Tetranychus urticae and T.kanzawai, are both highly polyphagous and occur widely in Japan. This study compared the variation in diapause characteristics of the two species and determined the phylogenetic relationship between the two forms of T.urticae (green and red) and also between those of T.kanzawai (K and T).The diapause of Japanese T.uticae is highly variable and diapause percentage ranged from 100% in the north to nearly zero in southwestern Honshu and Kyushu, but no difference was detected among populations from different host types. On the other hand, T.kanzawai had a strong diapause capacity and populations in the 4 main islands of Japan exhibited nearly 100% diapause. However, diapause percentage drastically decreased in populations from Okinawa Islands. T.kanzawai populations derived from tea had weaker diapause capacity than those derived from herbaceous and deciduous hosts.Analysis of COI gene (mitochondrial cytochrome oxidase subunit I) showed that the two forms of T.urticae were recognized in a single cluster and the existence of haplotypes with phylogenetical divergence suggested that multiple accidental immigrations of the mite had occurred in Japan.The phylogenetic trees constructed using the DNA sequences (COI and ITS1) failed to clearly distinguish between the two types T and K of T.kanzawai. The results suggested that the T types were derived from the K type.

两种叶螨：二斑叶螨（Tetranychus urticae）和叶螨（T.kanzawai）都是高度杂食性的，在日本广泛存在。本研究比较了这两个物种滞育特征的变化，并确定了两种形式的二斑荨麻疹（绿色和红色）之间以及红斑荨麻疹（K和T）之间的系统发育关系。日本斑鸠菊的滞育变化很大，滞育率从北部的100%到本州西南部和九州的接近零，但不同寄主的种群之间没有检测到差异。类型。另一方面，T.kanzawai具有很强的滞育能力，日本4个主要岛屿的种群表现出近100%的滞育率。然而，冲绳群岛人口的滞育率急剧下降。来自茶树的T.kanzawai种群的滞育能力比来自草本和落叶寄主的种群要弱。COI基因（线粒体细胞色素氧化酶亚基I）分析表明，T.urticae的两种形式被识别在一个簇中，并且具有系统发育差异的单倍型的存在表明该螨在日本。使用DNA序列（COI和ITS1）构建的系统发育树未能清楚地区分T.kanzawai的T和K两种类型。结果表明T型是从K型衍生而来的。