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Ecological and molecular phylogenetic analysis of the distribution expansion process of spider mites in Southeast and East Asia

东南亚和东亚红蜘蛛分布扩展过程的生态和分子系统发育分析

基本信息

批准号：
16405022
负责人：
TAKAFUJI Akio
金额：
$ 7.3万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16405022/
关键词：
Spider mites Diapause COI sequences Tetranychus urticae Tetranychus kanzawai Phylogenetic relationship Distribution expansion COI 塩基配列分子系統樹 DNAバーコード遺伝的分化 DNA アジア地理的変異

项目摘要

We studied the variation in diapause capacity and the mitochondrial DNA (COI) variation in two species of spider mites (Tetranychus urticae and T.kanzawai) for populations collected from Southeast and East Asia, to elucidate their distribution expansion patterns over the area. Japanese T.uritice (green form) populations showed a clinal change in diapause percentage and populations in the South had a very low diapause capacity. This low diapause capacity was also observed in all populations from Southeast and East Asia. The mites are known to originate from the Mediterranean areas and to have expanded its distribution range over the world within a short period. The present phylogenetic analysis based on COI gene confirmed that all populations from the areas studied are included in a single cluster with very little variation in the COI sequences. The results combined showed that T.urticae is an invasive species in Asia, including Japan, and in the process of distribution expansion the mi … More tes lost its diapause capacity, while they obtained high dispersal capacity, to adjust warm climates with continuous food availability.Most Japanese T.kanzawai populations, except those from Okinawa Islands, exhibited diapause percentages higher than 90%. Diapause percentages varied greatly among populations from Taiwan. This genetically diversified diapause capacity was also observed in populations from northern Vietnam. While populations from Thailand, including those from in the North, showed very low diapause capacity, populations from West Jawa, Indonesia and those from Manila, Philippines maintained high diapause capacity, in spite of their distribution in the tropics. Thus, in T.kanzawai no clear relationship was observed between dipause capacity and latitude and diapause was not subject to natural selection. The phylogenetic analysis based on the COI sequences showed that all populations from Honshu and southward, including those from Korea, Taiwan, Vietnam, and Thailand were genetically homogeneous and formed a single clade. However, populations from northern Hokkaido had 13 haplotypes, thus showing a comparatively high genetic differentiation. The results above implied that T.kanzawai originates in northern area and has expanded its distribution range to southern areas very recently. Less

我们研究了东南亚和东亚两种叶螨（二斑叶螨和神泽叶螨）的滞育能力和线粒体DNA（COI）变异，以阐明它们在该地区的分布扩展模式。日本野螟（绿色型）种群滞育率呈梯度变化，南方种群滞育能力很低。在东南亚和东亚的所有种群中也观察到这种低滞育能力。已知该螨起源于地中海地区，并在短时间内将其分布范围扩大到世界各地。目前基于COI基因的系统发育分析证实，所有的人口从研究的地区包括在一个单一的集群，在COI序列的变化很小。综合以上结果表明，二斑叶螨在包括日本在内的亚洲地区是一种入侵物种，在分布扩展的过程中， ...更多信息除冲绳外，大部分日本神泽赤眼蜂种群的滞育率均在90%以上。滞育率在台湾种群间差异很大。这种遗传多样性滞育能力也观察到从北方越南的人口。虽然来自泰国的种群，包括来自北方的种群，表现出非常低的滞育能力，但来自印度尼西亚西爪哇和菲律宾马尼拉的种群，尽管分布在热带，却保持着很高的滞育能力。因此，在T.kanzawai没有观察到明显的关系，滞育能力和纬度和滞育不受自然选择。基于COI序列的系统发育分析表明，所有的人口从本州和南方，包括那些来自韩国，台湾，越南和泰国的遗传同质，形成一个单一的分支。而来自北方北海道的群体则有13种单倍型，表现出较高的遗传分化。以上结果表明，神泽赤眼蜂起源于北方地区，近年来已向南方地区扩展。少