权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

molecular basis for the virion formation of influenza virus

流感病毒病毒粒子形成的分子基础

基本信息

批准号：
10470082
负责人：
NAKAJIMA Katsuhisa
金额：
$ 8.45万
依托单位：
Nagoya City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10470082/
关键词：
Influenza virus virion formation HA protein neuraminidase desialidation GG167 インフルエンザウィルス

项目摘要

We analyzed the role of neuraminidase (NA) on the haemadsorption of haemagglutinin (HA) protein of influenza B virus. The influenza B virus mutant ts-7 had temperature-sensitive mutation in the NA protein. At high temperature, infected cells did not exhibit haemadsorption activity, but addition of bacterial neuraminidase restored haemadsorption activity. Experiments with point-mutated HA cDNAs of B virus showed that two N-acetyl glycosylation sites at amino acid residues 160 and 217 were responsible for the inability of the HA protein to adsorb to erythrocytes. These results indicated that haemadsorption by the HA protein of influenza B virus required involvement of neuraminidase. Because the neuraminidase inhibitor Zanamivir was reported not to penetrate cells, we investigated the action of this inhibitor and found that Zanamivir inhibited haemadsorption on MDCK cells infected with B virus. These results indicated that desialidation of HA was done by NA after two proteins moved to cel … More l surface. From the reassortment experiments between A/Aichi/92 and A/WSN/33 (WSN) viruses, two different phenotype viruses which contained HA gene from A/Aichi/ 92 virus and the NA gene from WSN virus were obtained. PW15 viruse agglutinated chicken red blood cells (CRBC), while PW10 virus did not. However, the expressed HA proteins of these viruses did not adsorb CRBC.The difference in gene constellation between PW15 and PW10 viruses was the membrane protein (M) gene. The former had the M gene from A/Aichi/4/92 virus and the latter had that from WSN virus. In PW15-infected cells, haemadsorption of CRBC was observed 30 min later than that of goose red blood cells and the M1 protein migrated from the nucleus to the cytoplasm 30 min earlier than adsorption of CRBC was observed. On the other hand, in PW1O-infected cells, haemadsorption of CRBC was not observed through the virus replication and the M1 protein stayed in the nucleus after HA and NA activities reached maximum levels. Therefore M1 protein may contribute the desialidation of HA protein by NA protein. Less

我们分析了神经氨酸酶(NA)在乙型流感病毒血凝素(HA)蛋白血吸附中的作用。B型流感病毒突变株ts-7在NA蛋白上有温度敏感型突变。在高温下，感染细胞不表现出吸血活性，但添加细菌神经氨酸酶恢复了吸血活性。对B病毒HA基因的点突变实验表明，第160和217位氨基酸残基上的两个N-乙酰糖基化位点是导致HA蛋白不能吸附到红细胞上的原因。这些结果表明，乙型流感病毒HA蛋白的血液吸附需要神经氨酸酶的参与。由于神经氨酸酶抑制剂扎那米韦不能穿透细胞，我们研究了这种抑制剂的作用，发现扎那米韦抑制了感染B病毒的MDCK细胞的血吸附。这些结果表明，透明质酸是在两个蛋白质移动到细胞…后被NA脱除的。L更是浮出水面。通过A/Aichi/92和A/WSN/33(WSN)病毒的重组实验，获得了A/Aichi/92病毒HA基因和WSN病毒NA基因的两种不同表型病毒。PW15病毒能凝集鸡红细胞，而PW10病毒不凝集鸡红细胞。然而，这些病毒表达的HA蛋白不吸附CRBC。PW15和PW10病毒基因星座的不同在于膜蛋白(M)基因。前者含有A/Aichi/4/92病毒的M基因，后者含有WSN病毒的M基因。在PW15感染的细胞中，观察到CRBC的血液吸附比鹅红细胞晚30min，并且观察到M1蛋白比CRBC的吸附提前30min从细胞核迁移到细胞质。另一方面，在PW10感染的细胞中，没有通过病毒复制观察到CRBC的血吸附，在HA和NA活性达到最大水平后，M1蛋白停留在细胞核中。因此，M1蛋白可能参与了NA蛋白对HA蛋白的脱脂作用。较少