The study on the molecular basis for neurovirulence of influenza virus.

我们以前报道过神经毒性甲型流感病毒株A/WSN/33对小鼠大脑黑质有很强的亲和力。在这个项目中，我们尝试了两个研究。(1)利用神经元-神经胶质共培养系统观察细胞对病毒感染的体外反应。病毒抗原严格局限于神经元细胞体及其神经突。我们可以得出结论，病毒优先感染神经元，主要是报道的th阳性神经元在SN体内感染。因此，体外培养系统对流感病毒的神经毒力研究具有重要意义。(2)我们检测了经鼻接种后神经毒性甲型流感病毒在脑内的免疫组织化学定位。我们观察到，在感染的早期阶段，甲型流感病毒入侵，可能通过血液传播，并特别影响儿茶酚胺能神经元。

we previously reported that the neurovirulent influenza A virus strain, A/WSN/33 has strong affinity for the substantia nigra in mouse brain. In this project we tried two study. (1)we observed the cellular response to viral infection in vitro with using a neuron-glia co-culture system. Viral antigens were strictly confined to the neuronal cell bodies and their neurites. We can conclude that the virus preferentially infects neurons, mainly TH-positive neurons in the SN as reported in vivo infection.. Therefore In vitro culture system is useful for the study of neurovirulence of influenza Virus. (2)We examined the immunohistochemical localization of neurovirulent influenza A virus in the brain after intranasal inoculation. We observed in the early phase of infection, influenza A virus invades, probably through hematogenous spread, and particularly affects catecholaminergic neurons.