The study on the molecular basis for neurovirulence of influenza virus.

我们曾报道甲型流感病毒神经毒株A/WSN/33与小鼠脑内黑质有很强的亲和力。在这个项目中，我们尝试了两项研究。(1)采用神经元-神经胶质细胞共培养系统，观察细胞对病毒感染的体外反应。病毒抗原仅限于神经元胞体及其突起。我们可以得出结论，病毒优先感染神经元，主要是黑质中的TH阳性神经元，正如在体内感染所报道的那样。因此，体外培养体系可用于流感病毒神经毒力的研究。(2)鼻腔接种甲型流感强毒后，观察其在脑内的免疫组织化学定位。我们观察到，在感染的早期阶段，甲型流感病毒可能通过血液传播入侵，特别是对儿茶酚胺能神经元的影响。

we previously reported that the neurovirulent influenza A virus strain, A/WSN/33 has strong affinity for the substantia nigra in mouse brain. In this project we tried two study. (1)we observed the cellular response to viral infection in vitro with using a neuron-glia co-culture system. Viral antigens were strictly confined to the neuronal cell bodies and their neurites. We can conclude that the virus preferentially infects neurons, mainly TH-positive neurons in the SN as reported in vivo infection.. Therefore In vitro culture system is useful for the study of neurovirulence of influenza Virus. (2)We examined the immunohistochemical localization of neurovirulent influenza A virus in the brain after intranasal inoculation. We observed in the early phase of infection, influenza A virus invades, probably through hematogenous spread, and particularly affects catecholaminergic neurons.