The study on the molecular basis for neurovirulence of influenza virus.

我们先前报道了神经毒性甲型流感病毒株A/WSN/33对小鼠脑中的黑质具有强亲和力。在这个项目中，我们尝试了两项研究。(1)we采用神经元-胶质细胞共培养系统，观察病毒感染对细胞的影响。病毒抗原严格限于神经元细胞体和它们的神经突。我们可以得出结论，病毒优先感染神经元，主要是SN中的TH阳性神经元，如体内感染所报道的。因此，体外培养系统可用于流感病毒神经毒力的研究。(2)We研究了鼻内接种后神经毒性甲型流感病毒在脑中的免疫组织化学定位。我们观察到在感染的早期阶段，甲型流感病毒可能通过血行传播侵入，特别是影响儿茶酚胺能神经元。

we previously reported that the neurovirulent influenza A virus strain, A/WSN/33 has strong affinity for the substantia nigra in mouse brain. In this project we tried two study. (1)we observed the cellular response to viral infection in vitro with using a neuron-glia co-culture system. Viral antigens were strictly confined to the neuronal cell bodies and their neurites. We can conclude that the virus preferentially infects neurons, mainly TH-positive neurons in the SN as reported in vivo infection.. Therefore In vitro culture system is useful for the study of neurovirulence of influenza Virus. (2)We examined the immunohistochemical localization of neurovirulent influenza A virus in the brain after intranasal inoculation. We observed in the early phase of infection, influenza A virus invades, probably through hematogenous spread, and particularly affects catecholaminergic neurons.