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Influence of habitual exercise and circumstantial stresses on non-specific immunity and oxidant-antioxidant balance

习惯性运动和环境压力对非特异性免疫和氧化-抗氧化平衡的影响

基本信息

批准号：
10470101
负责人：
KUMAE Takashi
金额：
$ 1.92万
依托单位：
National Institute of Public Health
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

We presented the results of basic research using rats as an experimental animal at many scientific congresses. The stresses, forced or voluntary exercise, sleep disturbance, and isolation, were loaded to mature rats. The forced and voluntary exercise influenced body weights, however, the producing ability of reactive oxygen species (ROS) from alveolar macrophages was almost not affected by the stresses. ROS was measured by a parallel luminescent measuring system using a 96-well microplate. To develop an index to measure alveolar macrophage activity, the system was remodeled for fluorescence measurement. There were few significant differences among the stress groups on the oxidant-antioxidant balance in mitochondrial and microsomal fractions of rat lungs. These results suggest that non-specific immunity and oxidant-antioxidant balance in lungs are resistant to the stresses loaded after maturation.To clarify the subjects' circumstances toward forced or voluntary, we surveyed the health, … More psychological, and food-intake statuses in athletes and reported the results to the scientific journals. The effect of endurance training during a 1-month summer camp on the non-specific immunity was investigated and published in Int. J.Sports Med. The results suggest that the non-specific immunity is not directly impaired by the summer camp. The cytotoxic activities of natural killer cells of joggers were not affected by endurance running during a month-long distance run competition. A measurement method for a total antioxidant activity of serum was established to estimate the in vivo oxidant-antioxidant balance and we reported the mechanistic approach and application of the serum total antioxidant activity. The serum total antioxidant activity is a trapping capacity of ROS.The influence of daily-exercise on the oxidant-antioxidant balance of aged residents (over 65 years old) was estimated by serum, however, no significant influence of daily-exeercise was observed. Intensity of daily-exercise may be too low to affect the oxidant-antioxidant balance. Less

我们在许多科学大会上展示了以大鼠为实验动物的基础研究结果。对成年大鼠施加强迫或自愿运动、睡眠障碍和隔离等应激。强迫运动和自愿运动影响体重，但肺泡巨噬细胞产生ROS的能力几乎不受应激的影响。ROS由96孔微孔板的平行发光测量系统测量。为了开发一种测量肺泡巨噬细胞活性的指数，该系统被改装为荧光测量。各应激组大鼠肺线粒体和微粒体的氧化-抗氧化平衡无明显差异。这些结果表明，肺中的非特异性免疫和氧化-抗氧化平衡对成熟后的应激负荷具有抵抗力。为了澄清受试者被迫或自愿的情况，我们调查了健康，…更多的运动员的心理和食物摄入状况，并将结果报告给科学期刊。对1个月夏令营耐力训练对非特异性免疫功能的影响进行了研究，并发表在《国际》杂志上。J.Sports Med。提示夏令营对机体非特异性免疫功能无直接损害作用。在长达一个月的长跑比赛中，耐力跑不影响慢跑者自然杀伤细胞的细胞毒活性。建立了一种测定血清总抗氧化活性的方法，以评价体内氧化-抗氧化平衡，并报道了血清总抗氧化活性的机理及应用。每日运动对老年居民(65岁以上)氧化-抗氧化平衡的影响是通过血清来评估的，而日常运动对老年居民氧化-抗氧化平衡的影响不明显。日常锻炼的强度可能太低，不会影响氧化-抗氧化平衡。较少