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Study of endurance training effects on anti-oxidative activity.

耐力训练对抗氧化活性影响的研究。

基本信息

批准号：
14380022
负责人：
KUMAE Takashi
金额：
$ 4.67万
依托单位：
Incorporated administrative agency National Institute of Health and Nutrition
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

One characteristic point of this study is that we establish a simple and convenient method to measure levels of total anti-oxidative activity(TAA) in serum based upon a hypothesis that antioxidants will act synergistically and cooperatively in vivo. The other point in this study is to establish a reliable measurement method of reactive oxygen species(ROS) generated by phagocytes.The antioxidants in a rat broncho-alveolar lavage fluid(BALF) were measured to estimate oxidative stress. It is thought that in BALF, the protein and the thiobarbituric acid reactive materials(TBAR) level are good marker for acute and long term stress, respectively. Different effects between forced and voluntary training on rats were revealed by measurements of ROS generating capability of alveolar macrophage(AM) and levels of IL-1β,IFNγ,and TNFα,in AM cultured mediums.Effects of marathon race on civilian runners were investigated. Significant increases were observed in the neutrophil numbers and levels of IL-6,IL-8,and G-CSF after the race. The nuetrophilic functions and the expressions of CD11b and CD16 were measured on runners run completely the race. Results suggest that the nuetrophilic functions are suppressed after the race and increment of neutrophil number may be compensatory effects of decreased functions. The antioxidants, TAA, and cytokine levels of female long-distance runner were measured to investigate effects of a summer camp. Significant positive correlation was observed between TAA and TBAR levels. According to changes of cytokine levels, systemic inflammatory response is suppressed and Th2 activity may be down regulated. Effects of habitual exercise on serum antioxidant levels in older residents in a rural area were studied. Significant positive correlation is observed between TAA and TBAR levels in a group of males with a habitual physical exercise.These results suggest that TAA is a useful marker to estimate an anti-oxidative status in serum.

本研究的一个特点是，我们建立了一个简单方便的方法来测量血清中的总抗氧化活性（TAA）的水平的基础上的假设，抗氧化剂将在体内发挥协同作用和合作。本研究的另一个目的是建立一种可靠的测定吞噬细胞产生活性氧（ROS）的方法，通过测定大鼠支气管肺泡灌洗液（BALF）中的抗氧化剂来评价氧化应激。认为BALF中蛋白质和硫代巴比妥酸反应物质（TBAR）水平分别是急性和长期应激的良好标志。通过测定大鼠肺泡巨噬细胞（AM）活性氧（ROS）产生能力及AM培养液中IL-1β、IFNγ、TNFα水平，揭示了强制训练与自愿训练对大鼠的不同影响，并探讨了马拉松比赛对普通运动员的影响。在比赛后观察到中性粒细胞数量和IL-6、IL-8和G-CSF水平显著增加。对跑完全程的运动员进行了外周血淋巴细胞的功能检测和CD 11b、CD 16的表达。结果提示，赛跑后中性粒细胞功能受到抑制，中性粒细胞数量的增加可能是对功能下降的代偿作用。为探讨夏令营对女子长跑运动员抗氧化能力、TAA和细胞因子水平的影响。TAA与TBAR水平呈显著正相关。根据细胞因子水平的变化，全身炎症反应受到抑制，Th 2活性可能下调。研究了农村老年人运动习惯对血清抗氧化水平的影响。在一组有运动习惯的男性中，TAA与TBAR水平呈显著正相关，提示TAA是一个评价血清抗氧化状态的有用指标。