ESTIMATION OF POSTMORTEM INTERVAL BY POSTMORTEM BIOCHEMICAL CHANGES

通过死后生化变化估计死后间隔

• 批准号: 10470118
• 负责人: MISAWA Shogo
• 金额: $ 7.23万
• 依托单位: UNIVERSITY OF TSUKUBA
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10470118/
• 关键词: post-mortem interval; vascular endothelial growth factor (VEGF); hypoxia; forensic science; ウェスタン・ブロッティング; 酸素・金属応答遺伝子; ウエスタン・ブロッティング; スーパーファミリー

Estimation of the post-mortem interval (PMI) is one of the most important tasks in forensic medicine. Five autopsy organ tissues such as brain, lungs, heart, liver and kidneys were taken at the time of forensic autopsy from 20 known PMI cases with a range of postmortem intervals ranging from 1 to 120 hrs (the mean was 25.81 hrs) and the time-course of vascular endothelial growth factor (VEGF) expression was measured. The human hepatoma-derived Hep 3B cell line was used as a control.The levels of VEGF increased linearly with the PMI up to 20 hrs in lung (r=0.95 and in kidney (r=0.89), and up to 15 hrs PMI in liver (r=0.88). The VEGF levels fell after 24 hrs PMI, and then remained stable. In brain, the levels of VEGF started to increase after 24 hrs PMI and increased linearly with PMI up to 40 hrs in brain (r=0.94) and then begin to fall. In heart, there was no clear correlation between the PMI and VEGF level. Some variations occurred in selected cases, such as the infant and asphyxial deaths.In conclusion, measurement of hypoxia-inducible levels of VEGF in various body organs appears to be a useful method of estimating the PMI up to 24 hrs in forensic medicine and pathophysiology. This method is alsoprobably applicable in ischaemia in clinical and basic medicine.

死亡时间推断是法医学研究的重要内容之一。对20例已知的PMI尸检标本进行尸检时，取脑、肺、心、肝、肾等5种脏器组织，测定其血管内皮生长因子（VEGF）表达的时程。以人肝癌细胞系Hep 3B为对照，肺组织中VEGF表达与PMI呈线性关系，相关系数分别为0.95、0.89和0.88，而肝组织中VEGF表达与PMI呈线性关系，相关系数分别为0.88和0.95。PMI 24小时后VEGF水平下降，然后保持稳定。脑组织中VEGF水平在PMI 24小时后开始升高，40小时后与PMI呈线性关系（r=0.94），然后开始下降。在心脏中，PMI和VEGF水平之间没有明确的相关性。一些变化发生在选定的情况下，如婴儿和窒息deaths.In结论，测量缺氧诱导的VEGF水平在不同的身体器官似乎是一个有用的方法估计PMI到24小时法医学和病理生理学。该方法在临床和基础医学上也可能适用于缺血性疾病。

项目成果

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阪本奈美子・三澤章吾 他: "乳幼児突然死症候群におけるカルシトニン遺伝子関連ペプチド含有肺神経内分泌細胞の分布の免疫組織化学的検討" 日法医誌. 52(1). 27-36 (1998)

Namiko Sakamoto、Shogo Misawa 等：“婴儿猝死综合征中含有降钙素基因相关肽的肺神经内分泌细胞分布的免疫组织化学研究”日本法医学杂志 52(1) (1998)。

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