Synaptic relationship between primary, afferent newe terminals and interneurons in the spinal trigeminal nucleus caudalis

三叉神经尾核初级、传入神经末梢和中间神经元之间的突触关系

• 批准号: 10470382
• 负责人: HIURA Akio
• 金额: $ 1.98万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10470382/
• 关键词: Synapses; Primary afferent neuron; Substantia gelatinosa; Interneuron; Neurophysiological study; Synapses; Primary afferent; Interneuron; Substantia gelatinosa; Spinal trigeminal nucleus; Svnapses; Primarv afferent; Spinal trigeminalnucleus

The aim of this study is to identify the interneurons responding to the electrical stimuli peculiar to convey nociceptive information in the substantia gelatinosa of the mouse superficial dorsal horn. The second is to, investigate the synaptic relation of nociceptive primary afferent nerve (PAN) terminals to inhibitory interneurons such as GABAergic, thereby prove the flow of impulse from the PAN terminals to GABAergic interneurons. Taking out the spinal cord or medulla oblongata, the specimens were coronally cut at 500 μm thickness with microslicer and put into the chamber continuously circulated with medium (PH7.2 〜 7.4) adjusted the temperature at 37℃, including 95 % O_2 and 5 % CO_2.Under the above ideal conditions, the glass electrode was inserted into the substantia gelatinosa. But now the resting potentials of the interneurons could not be obtained. Probably, the neurons were dying during the experiment. Thus, we have no data in this study, however, we have at least prepared the circumferential conditions to carry out the study. We should continue this study, overcome the problems and would get some of the interesting results in near future.

本研究的目的是鉴定小鼠浅表背角明胶质中对传递伤害性信息的特殊电刺激作出反应的中间神经元。二是研究伤害性初级传入神经（PAN）末梢与GABAergic等抑制性中间神经元的突触关系，从而证明脉冲从PAN末梢流向GABAergic中间神经元。取出脊髓或延髓，用显微切片机冠状切开500 μm厚度的标本，放入调温为37℃，95% O_2和5% CO_2的培养液（PH7.2 ~ 7.4）中连续循环。在上述理想条件下，将玻璃电极插入明胶物质中。但目前还无法获得中间神经元的静息电位。也许，在实验过程中神经元正在死亡。因此，我们在这项研究中没有数据，但是，我们至少准备了进行研究的周向条件。我们应该继续这项研究，克服这些问题，在不久的将来会得到一些有趣的结果。

项目成果

A.Hiura: "Cell death of primary afferent nerve cells in neonatal mice treated with capsaisin"Anatomical Science International. 77. 31-34 (2002)

A.Hiura：“用辣椒素治疗的新生小鼠中初级传入神经细胞的细胞死亡”国际解剖科学。

A.Hiura: "Neuroanatomical effects of capsaisin on the primary afferent neurons"Arch.Histol.Cytol. 63(3). 199-215 (2000)

A.Hiura：“辣椒素对初级传入神经元的神经解剖学影响”Arch.Histol.Cytol。

A.Hiura: "FRAP-positive and capsicin-sensitive terminals in the substantia gelatinosa of the mouse spinal trigeminal nucleus caudalis"Okajimas Folia Anat.Jpn. 76(1). 30-40 (1999)

A.Hiura：“小鼠脊髓三叉神经尾核胶质中的 FRAP 阳性和辣椒素敏感末端”Okajimas Folia Anat.Jpn。

中川弘: "新生存期にカプサイシン投与したマウスの侵害熱刺激応答に対する抗NGFとNGFの影響"日薬理誌. 116. 93-100 (2000)

Hiroshi Nakakawa：“抗 NGF 和 NGF 对新生儿期服用辣椒素的小鼠对有害热刺激反应的影响”《日本药理学杂志》116. 93-100 (2000)。

Hiura,A.: "Cell death of primary afferent nerve cells in neonatal mice treated with capsaisin"Anatomical Science International. 77. 31-34 (2002)

Hiura,A.：“用辣椒素处理的新生小鼠初级传入神经细胞的细胞死亡”国际解剖科学。