Synaptic relationship between primary, afferent newe terminals and interneurons in the spinal trigeminal nucleus caudalis

本研究的目的是鉴定小鼠背角浅层胶状质中对电刺激产生反应的中间神经元，这些中间神经元是传递伤害性信息的特有神经元。二是研究伤害性初级传入神经（PAN）终末与抑制性中间神经元（如GABA能）的突触联系，从而证实PAN终末向GABA能中间神经元的冲动传递。取脊髓或延髓标本，用显微切片机冠状面切至500 μ m厚，置于pH值为7.2~7.4、温度为37 ℃、含95%O_2和5%CO_2的介质中，在上述理想条件下，将玻璃电极插入胶状质。但现在中间神经元的静息电位无法获得。很可能，神经元在实验过程中死亡。因此，我们在本研究中没有数据，但是，我们至少准备了进行研究的周向条件。我们应该继续这项研究，克服这些问题，并将在不久的将来得到一些有趣的结果。

The aim of this study is to identify the interneurons responding to the electrical stimuli peculiar to convey nociceptive information in the substantia gelatinosa of the mouse superficial dorsal horn. The second is to, investigate the synaptic relation of nociceptive primary afferent nerve (PAN) terminals to inhibitory interneurons such as GABAergic, thereby prove the flow of impulse from the PAN terminals to GABAergic interneurons. Taking out the spinal cord or medulla oblongata, the specimens were coronally cut at 500 μm thickness with microslicer and put into the chamber continuously circulated with medium (PH7.2 〜 7.4) adjusted the temperature at 37℃, including 95 % O_2 and 5 % CO_2.Under the above ideal conditions, the glass electrode was inserted into the substantia gelatinosa. But now the resting potentials of the interneurons could not be obtained. Probably, the neurons were dying during the experiment. Thus, we have no data in this study, however, we have at least prepared the circumferential conditions to carry out the study. We should continue this study, overcome the problems and would get some of the interesting results in near future.