Basic morphological studies on the tooth pain transmission.

牙痛传递的基础形态学研究。

基本信息

  • 批准号:
    06671814
  • 负责人:
  • 金额:
    $ 1.41万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
  • 财政年份:
    1994
  • 资助国家:
    日本
  • 起止时间:
    1994 至 1995
  • 项目状态:
    已结题

项目摘要

We traced the trigeminal sensory neurons with HRP injected into the pulp of mouse lower incisors. Two adult ICR mice were anesthetized with Nembutal. The lower incisor on one side was then cut with a dental disk in the region of tooth eruption from the mandible. Ten microliter of 5% WGA-HRP dissolved in saline was injected into the pulp cavity by microsyringe. The animals were reanesthetized 48 hours later and perfused through the left cardiac ventricle first with saline and then with 200ml of 1% glutaraldehyde-0.5% paraformaldehyde in phosphate buffer(pH7.4). The ipsilateral trigeminal ganglion and brain stem were dissected out, re-fixed in the same fixative for 2 hours at 4゚C,and stored in buffered 10% sucrose solution at 4゚C.Frozen sections were cut at 30 mum with a Cryostat and rinsed in a solution of 3,3', 5,5'-tetramethylbenzidine and 0.3% H_2O_2. Large diameter of trigeminal ganglion neurons (total : 917 cells) from two animals were measured with ocular micrometer. The size of the mouse trigeminal ganglion neurons varied from 7 to 40 mum in diameter, having a peak between 20 and 25 mum. The HRP-labelled neurons were large, being 20-35mum in diameter. No HRP-incorporated neurons were seen in the brain stem, especially in the mesencephalic nucleus. These results directly or indirectly indicate that larger sensory neurons may give rise to unmyelinated fibers.Recently, since NO is thought of as a sensory neurotransmitter, we studied the distribution of the ANDPH-diaphorase (NADPH-d, NO synthetic enzyme) in the pulps of lower incisor and molar of the rat. As a result, many NADPH-d positive nerve fibers were seen in the plexus of Rushkow and dentinal tubules, running through the odontoblastic layr. Thus, the results suggest that the pain perception in the dentin and pulp is closlely related to NO-positive nerve fibers.
将HRP注射到小鼠下切牙牙髓内,追踪三叉神经感觉神经元。用Nembutal麻醉两只成年ICR小鼠。然后在下颌出牙区用牙盘切割一侧的下门牙。用微量注射器将10微升5%WGA-HRP溶于生理盐水注入牙髓腔内。48小时后再次麻醉,先用生理盐水经左心室灌注,然后用1%戊二醛-0.5%多聚甲醛200ml磷酸盐缓冲液(pH7.4)灌流。取同侧三叉神经节和脑干,在4゚C下用相同的固定剂重新固定2小时,保存在4゚C的10%蔗糖缓冲液中。冰冻切片在30 mm恒温器上切开,用3,3‘,5,5’-四甲基联胺和0.3%H_2O_2溶液冲洗。小鼠三叉神经节神经元的直径从7到40微米不等,峰值在20到25微米之间。HRP标记的神经元较大,直径为20~35微米。在脑干,尤其是中脑核内未见HRP阳性神经元。这些结果直接或间接地表明较大的感觉神经元可能产生无髓纤维。最近,由于NO被认为是一种感觉神经递质,我们研究了ANDPH-黄递酶(NADPH-d,NO合成酶)在大鼠下切牙和磨牙牙髓中的分布。结果显示,在Rushkow神经丛和牙本质小管丛中可见大量NADPH-d阳性神经纤维,贯穿成牙本质细胞层。因此,这些结果表明,牙本质和牙髓中的痛觉与NO阳性神经纤维密切相关。

项目成果

期刊论文数量(24)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Ishizuka,H.and Hiura,A.: "A light and electron microscopic study on pulpal nerve fibers in the lower incisor of the mouse." Arch.Histol.Cytol.55(2). 167-178 (1992)
Ishizuka,H. 和 Hiura,A.:“对小鼠下切牙牙髓神经纤维的光和电子显微镜研究。”
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    0
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Hiura, A., Nasu, F., Kuwahara, M.& Ishizuka, H.: "Large trigeminal sensory neurons innervate the mouse lower incisor pulp." Shikoku Dent.Res.7(2). 193-195 (1994)
日浦,A.,那须,F.,桑原,M.
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    0
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樋浦明夫、石塚寛、大田美香: "血漿漏出と痒み誘発における侵害受容性知覚神経とマスト細胞の役割" 東邦医学会雑誌. 42. 307-320 (1995)
Akio Hiura、Hiroshi Ishizuka、Mika Ota:“伤害性感觉神经和肥大细胞在血浆渗漏和瘙痒诱导中的作用”东邦医学会杂志 42. 307-320 (1995)。
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    0
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Ishizuka,H.and Hiura,A.: "Quantitative electron-microscopic analyses of pulpal nerve fibres in the mouse lower inoisor after neonatal capsaicin treatment." Archs oral Biol.37(12). 1085-1090 (1992)
Ishizuka, H. 和 Hiura, A.:“新生儿辣椒素治疗后小鼠下肌牙髓神经纤维的定量电子显微镜分析。”
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    0
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HIURA Akio其他文献

HIURA Akio的其他文献

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{{ truncateString('HIURA Akio', 18)}}的其他基金

Synaptic relationship between primary, afferent newe terminals and interneurons in the spinal trigeminal nucleus caudalis
三叉神经尾核初级、传入神经末梢和中间神经元之间的突触关系
  • 批准号:
    10470382
  • 财政年份:
    1998
  • 资助金额:
    $ 1.41万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)

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牙髓炎中三叉神经节神经元与成牙本质细胞之间逆行通讯对成牙本质细胞功能的调节
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    22K09972
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    2022
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Understanding trigeminal ganglion development through the lens of Familial Dysautonomia
从家族性自主神经功能障碍的角度了解三叉神经节的发育
  • 批准号:
    10432307
  • 财政年份:
    2022
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Understanding trigeminal ganglion development through the lens of Familial Dysautonomia
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三叉神经节在感觉处理中的作用
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Macrophages in trigeminal ganglion contributes to the ectopic orofacial pain following inferior alveolar nerve injury
三叉神经节中的巨噬细胞导致下牙槽神经损伤后的异位口面部疼痛
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谷氨酸作为神经递质将能够通过三叉神经节控制口腔感觉
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大鼠三叉神经节感觉形态的三维分布
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    19K24151
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