Study on the relationship between PTHrP gene expression and differentiation of oral squamous cell carcinoma

我们发现甲状旁腺激素相关蛋白、甲状旁腺激素相关蛋白、1-34位氨基酸的免疫染色与口腔鳞癌角化和恶性程度的组织学分级有关。外科医生。1997年，京都)。目的：探讨PTHrP基因表达与PTHrP1-34免疫组织化学染色及角化程度的关系。本研究采用33例口腔鳞癌患者的活检标本，其中女性11例，男性22例，正常黏膜4例。用免疫组织化学方法(LSAB法)对5μm厚的石蜡切片进行1-34多克隆抗体的免疫组织化学染色和非同位素原位杂交。我们使用地高辛标记的寡核苷酸探针，它是对编码PTHrP6-16氨基酸序列的人PTHrP mRNA的补充。切片用Mayer‘s苏木精反染。甲状旁腺激素受体基因表达阳性30/33例(91%)。免疫组织化学染色将所有标本分为两组，一组为弱染色，另一组为强染色。虽然强染色组的阳性信号百分率(100%，13/13)高于弱染色组的85%(17/20)，但两者的表达很难区分，提示PTHrP的转录可能对口腔癌的角化或分化影响不大。PTHrP的翻译后加工可能是造成这一现象的主要原因。需要额外的研究来阐明这个问题。部分资助来自日本文部科学省的助学金(10470443号)。

We found that immunostain of parathyroid hormone related protein, PTHrP, amino acids 1-34 correlated with a histological grading for keratinization and malignancy of oral squamous cell carcinoma, OSCC (abstract, the Int.Association of Oral ＆ Maxillofac. Surgeons. 1997, Kyoto). The purpose was to determine whether the expressin of the mRNA of PTHrP is related to the immunohistochemical stains of PTHrP 1-34 and keratinization. Biopsy specimens from 33 patients, 11 females and 22 males, with OSCC and 4 normal mucosal specimens were used of this study. A previously described method of immunohistochemical staining (LSAB method) with polyclonal 1-34 antibodies and non-isotopic in situ hybridization were performed on 5 μm thickness paraffin sections. We used digoxigenin labeled oligonucleotide probe which was complement to the human PTHrP mRNA coding for the amino acid sequence PTHrP 6-16. The sections were counterstained with Mayer's hematoxylin. Positive signals of PTHrP mRNA were present in 30/33 (91%) specimens. All specimens were classified in two groups, one showed slight stains and another showed intense staining by the immunohistochemical investigation. Although the percentage(100%, 13/13) of positive signals of the mRNA in the intense stain group is higher than that(85%, 17/20) in the weak stain group, it was difficult to distinguish two groups by expression of mRNA.The results suggest that transcription of PTHrP might not affect much to keratinication or differentiation of oral cancer. Post translational processing of PTHrP may take a major role for this phenomenon. Additional study is required to elucidate this issue. Partially supported by a Grant-in-aid (No.10470443) from the Ministry of Education Science, and Culture of Japan.