The study of meintenance of bone metabolism in small animals

为了阐明骨质疏松症的病理机制，建立防治骨质疏松症的方法，有必要建立动物模型。因此，阐明骨代谢，包括改变骨矿物质含量（BMC）和骨矿物质密度（BMD）是重要的。因此，本研究采用Aloka DCS-600 R型DXA测定了不同饮食条件对骨量变化的影响。1998年，研究了钠（Na）摄入量对钙（Ca）和骨代谢的影响。在本实验中，即使动物的骨量明显较低，也能够通过DCS-600 R分析BMD。此外，研究结果还表明，摄入过量的钠会对钙和骨代谢产生负面影响。1999年，通过测量体内（使用活体大鼠）和体外（使用解剖后提取的骨）BMC和BMD的变化，研究了牛奶对骨生长的影响。DCS-600 R能准确、准确地测定刚开始工作的幼鼠骨密度，提示牛奶对生长期的骨有明显的促进作用。2000年，我们用DCS-600 R测定了PTH对低钙饮食大鼠骨代谢的影响。并对不同骨密度仪测定结果进行相关性分析。结果表明，PTH对维持骨代谢有积极作用。DCS-600 R型骨密度仪测定结果与其它骨密度仪测定结果呈正相关，三次实验结果表明DCS-600 R型骨密度仪测定骨密度是检测骨代谢的可靠方法。

It is nessesary using animal models to elucidate of pathology of osteoporosis and to establish the ways of treatment and prevention of osteoporosis. Thus, a clarification of bone metabolism, include changing bone mineral content (BMC) and bone mineral density (BMD), is important. Therefore, in this study the effects of the different diet conditions on changing bone mass were determined using the DXA by Aloka's DCS-600R.In 1998, the influence of sodium (Na) intakes on calcium (Ca) and bone metabolism were examined. In this experiment, it was able to analyze the BMD by DCS-600R, even though the animal had a markedly low bone mass. In addition to this, the results indicated that an excess Na intakes affected negatively on Ca and bone metabolism.In 1999, the effect of milk on bone growth was examined by measurement of changing BMC and BMD in vivo (using vital rats) and in vitro (using extracted bone after dissection). The BMD of the young rats just after wearning could analyze accurately and precisely by DCS-600R.Moreover, the results suggested that the milk could be markedly effective and valuable for bone in growing period.In 2000, the influence of PTH on bone metabolism in the rats fed low Ca diet was determined using the DCS-600R.In addition, the correlation of the results of BMC and BMD in different densitometer was also examined. The results indicated that the PTH could effect positively on meintaining bone metabolism. And there was a possitive correlation between the results of DCS-600R and other densitometer.The results of three experiments show the measurement of BMD by DCS-600R is reliable to examine bone metabolism.