Study on stomach cancer and liver cancer by Helicobacter pylori in feetion and sterigmatocystin or nitrite treatment in mongorian gerbils

蒙古沙鼠足部幽门螺杆菌与杂色曲霉素或亚硝酸盐治疗胃癌和肝癌的研究

• 批准号: 10557041
• 负责人: MISUMI Junichi
• 金额: $ 11.46万
• 依托单位: OITA MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10557041/
• 关键词: mongorian gerbils; Hericobacter Pylori; Sterigmatocystin; ritrite; Stomach cancer; liver cancer; gastorin; 砂ネズミ; sterigmatocystin; 胃癌; 食塩

In this study, the effects of H.pylori, H.pylori supernatants and ST on the stomach and liver were investigated in Mongolian gerbils. 9-week-aged 214 Mongolian gerbils were divided into five groups (1)control, (2) H.pylori, (3) H.pylori supernatants (4) H.pylori with ST(100 ppb) and (5) H.pylori with ST(1000 ppb) groups. H.pylori were administered at 5.6x10^<12> CFU/ml. H.pylori culture supernatants (containing toxin VacA, we call it toxin below) and ST (in the concentration of 100 ppb and 1000 ppb each) which were administered for a period of two years and four months to animals, were added to feed and mixed respectively. Serum IgG antibody was determined by ELISA, and serum gastrin was determined by RIA. Stomach was examined by histopathological method including HE, PAS and Alcian blue (PH 2.5) staining.(1) Infection rate of H. pylori (table 1) Infection rate of H.pylori of every group which was determined by serum IgG is as follows : H. pylori with ST (100 ppb) group, 83.3 % ; H.pyl … More ori with ST (1000 ppb) group, 72.2 % ; H.pylori group, 63.3 % ; Toxin group, 0 % ; control group, 0 %. There is no significant difference between H.pylori and H.pylori with ST groups. (2) Serum gastrin (table 2) Average value of serum gastrin in every group is : control group, 131.9 pg/ml ; toxin group, 126.0 pg/ml ; H.pylori group, 138.2 pg/ml ; H.pylori with ST (1000 ppb), 179.4 pg/ml ; H.pylori with ST (100 ppb) 156.8 pg/ml. The gastrin values of H.pylori with ST groups were higher than the other groups. There is a significant difference between H. pylori group and H.pylori with ST (1000 ppb) group. It is suggested that ST has some effects on gastrin excretion. (3) Pathology (Macroscopic findings) (table 1) The gastric mucosa of all gerbils in the control and toxin groups showed no visible changes. The gastric mucosa of most H.pylori infected gerbils appeared expanded and thickened in varying degrees, covered with abundant mucus, erosive lesions with bleeding, ulcer, or showed marked cancers. The gastric cancer incidence of every group was : (1) H.pylori with ST (100 ppb) group, 72.2 % ; (2) H.pylori with ST (1000 ppb) group, 63.9 % ; (3) H.pylori group, 40.9 % ; (4) toxin group, 0 % ; (5) control group, 0 %. The gastric cancer incidence of H.pylori administered groups is significantly higher than that of none administered groups. The gastric cancer incidence of H.pylori with ST groups is significantly higher than that of H.pylori group. These results suggest that H.pylori can induce gastric cancer, and that ST can enhance the effect of H. pylori. Less

本研究调查了幽门螺杆菌、幽门螺杆菌上清液和 ST 对蒙古沙鼠胃和肝脏的影响。将214只9周龄蒙古沙鼠分为五组：（1）对照组、（2）H.pylori组、（3）H.pylori上清组、（4）H.pylori ST（100 ppb）组和（5）H.pylori ST（1000 ppb）组。以5.6×10 12 CFU/ml施用幽门螺杆菌。将H.pylori培养上清液（含有毒素VacA，以下称为毒素）和ST（浓度分别为100ppb和1000ppb）分别添加到饲料中并混合，为期两年和四个月。血清IgG抗体采用ELISA法测定，血清胃泌素采用RIA法测定。胃组织病理学检查包括HE、PAS和阿尔新蓝(PH 2.5)染色。(1)H. pylori感染率(表1)血清IgG测定各组H. pylori感染率如下:H. pylori ST(100 ppb)组,83.3%;H. pylori ST(100 ppb)组,83.3%; H.pyl … More ori 含 ST (1000 ppb) 基团，72.2 %；幽门螺杆菌组，63.3%；毒素组，0%；对照组，0%。 H.pylori与ST组H.pylori无显着差异。 (2)血清胃泌素(表2)各组血清胃泌素平均值为：对照组131.9pg/m​​l；毒素组，126.0 pg/ml；幽门螺杆菌组，138.2 pg/ml；幽门螺杆菌，ST (1000 ppb)，179.4 pg/ml；幽门螺杆菌与 ST (100 ppb) 156.8 pg/ml。 ST组H.pylori胃泌素值高于其他组。 H. pylori组与H. pylori加ST(1000 ppb)组有显着差异。提示ST对胃泌素的排泄有一定影响。 (3)病理学(肉眼观察)(表1)对照组和毒素组所有沙鼠的胃粘膜均未见明显变化。大多数感染H.pylori的沙鼠胃粘膜出现不同程度的扩张、增厚，粘液较多，糜烂性病变伴出血、溃疡，或有明显癌变。各组胃癌发病率分别为：（1）H.pylori加ST（100ppb）组，72.2%； (2) ST (1000 ppb) 组幽门螺杆菌，63.9%； (3)幽门螺杆菌组，40.9%； (4)毒素组，0%； (5)对照组，0%。 H.pylori给药组的胃癌发病率显着高于未给药组。 ST组H.pylori胃癌发病率显着高于H.pylori组。这些结果表明H.pylori可以诱发胃癌，而ST可以增强H.pylori的作用。较少的