Study on stomach cancer by iodine deficiency and salt addition food in Helicobacter pylori infected mongorian gerbils.

蒙古沙鼠很容易感染幽门螺杆菌，由此产生的慢性活动性胃炎、消化性溃疡和肠化生类似于人类出现的病变。蒙古沙鼠感染幽门螺杆菌对胃癌发生分析有重要意义。我们拟用ⅰ和NaCl缺乏症感染幽门螺杆菌的蒙古沙鼠建立胃癌动物模型。研究对象为170只7周龄雄性蒙古沙鼠。将沙鼠随机分为8组：1)只给予正常饲料；2) H。幽门螺杆菌感染；3)I不足；4) H。幽门螺杆菌+I缺乏症；5)5% NaCl；6) H。幽门螺杆菌+5% NaCl；7)5% NaCl+I缺乏症；8) H。幽门螺杆菌+5% NaCl+I缺乏症。实验27个月后（2005年4月4日）处死。组织病理学改变（HE染色）高盐HP感染组（6组）眼底可见明显的炎性细胞浸润，HP感染组（2组）眼底可见异位幽门腺伴严重炎性细胞浸润。此外，2组和3组感染动物幽门黏膜细胞增殖明显加快。第3组和第8组感染动物幽门黏膜增生性改变，幽门腺体囊性扩张和糜烂。部分幽门腺多灶性侵及粘膜肌层并延伸至粘膜下层。增生层和粘膜下层可见淋巴细胞、巨噬细胞和中性粒细胞浸润，淋巴滤泡形成。另一方面，1组动物未见异常，而3组动物固有层黏膜严重损伤，腺体缺损。另外，我们制作了Alcian Blue染色剂和PAS染色剂。

Mongolian gerbils can be readily infected with H.pylori, and the resultant chronic active gastritis, peptic ulcers, and intestinal metaplasia resemble lesions appearing in humans. Mongolian gerbils infected with H.pylori are very useful for the analysis of gastric carcinogenesis.We intended to establish an animal model of stomach carcinogenesis using H.pylori-infected Mongolian gerbils with deficency I and NaCl. A total of 170 male, 7-week-old Mongolian gerbils were used for this study.Gerbiles were randomly assigned to eight groups : 1)only given normal feed ; 2)H.pylori infection ; 3)I deficiency ; 4)H.pylori+I deficiency ; 5)5% NaCl ; 6)H.pylori+5% NaCl ; 7)5% NaCl+I deficiency ; 8)H.pylori+5% NaCl+I deficiency. The animal were killed(2005/4/4) after 27 months of the experiment.Histopathological changes(HE stain)In the HP infection(group 6) with high salt, there was a evident inflammatory cell infiltration in the fundus and in HP infection(group 2) ectopic pyloric glands with a severe inflammatory cell infiltration were observed the fundic regions. In addition, the cell proliferation of the pyloric mucosa was markedly accelerated in the infected animals from groups 2 and 3. The infected animals from groups 3 and 8 revealed hyperplastic changes in the pyloric mucosa, cystic glandular dilation of the pyloric glands and erosions. Some of the pyloric glands penetrated the muscularis mucosae multifocally and extended into the submucosa. Infiltration of lymphocytes, macrophages and neutrophils was seen in the lamina proplia and submucosa with formation of lymphoid follicles. On the other hand, no abnormal findings were observed in animals from group 1, whereas a severe damage of the lamina propria mucosa with a defect of glands was found in animals from group 3.In addition, we made Alcian Blue stain and PAS stain.