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Study on stomach cancer by iodine deficiency and salt addition food in Helicobacter pylori infected mongorian gerbils.

幽门螺杆菌感染蒙古沙鼠缺碘和加盐食品引起胃癌的研究

基本信息

批准号：
14370125
负责人：
MISUMI Junichi
金额：
$ 8.9万
依托单位：
Oita University (2004)大分医科大学 (2002-2003)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

Mongolian gerbils can be readily infected with H.pylori, and the resultant chronic active gastritis, peptic ulcers, and intestinal metaplasia resemble lesions appearing in humans. Mongolian gerbils infected with H.pylori are very useful for the analysis of gastric carcinogenesis.We intended to establish an animal model of stomach carcinogenesis using H.pylori-infected Mongolian gerbils with deficency I and NaCl. A total of 170 male, 7-week-old Mongolian gerbils were used for this study.Gerbiles were randomly assigned to eight groups : 1)only given normal feed ; 2)H.pylori infection ; 3)I deficiency ; 4)H.pylori+I deficiency ; 5)5% NaCl ; 6)H.pylori+5% NaCl ; 7)5% NaCl+I deficiency ; 8)H.pylori+5% NaCl+I deficiency. The animal were killed(2005/4/4) after 27 months of the experiment.Histopathological changes(HE stain)In the HP infection(group 6) with high salt, there was a evident inflammatory cell infiltration in the fundus and in HP infection(group 2) ectopic pyloric glands with a severe inflammatory cell infiltration were observed the fundic regions. In addition, the cell proliferation of the pyloric mucosa was markedly accelerated in the infected animals from groups 2 and 3. The infected animals from groups 3 and 8 revealed hyperplastic changes in the pyloric mucosa, cystic glandular dilation of the pyloric glands and erosions. Some of the pyloric glands penetrated the muscularis mucosae multifocally and extended into the submucosa. Infiltration of lymphocytes, macrophages and neutrophils was seen in the lamina proplia and submucosa with formation of lymphoid follicles. On the other hand, no abnormal findings were observed in animals from group 1, whereas a severe damage of the lamina propria mucosa with a defect of glands was found in animals from group 3.In addition, we made Alcian Blue stain and PAS stain.

蒙古沙鼠很容易感染幽门螺杆菌，并由此产生慢性活动性胃炎、消化性溃疡和肠上皮化生，类似于人类出现的病变。幽门螺杆菌（H.pylori）感染的蒙古沙鼠在胃癌发生机制的研究中具有重要意义，本研究拟用H. pylori感染的蒙古沙鼠建立胃癌发生的动物模型。选用170只7周龄雄性长爪沙鼠，随机分为8组：1）正常饲料组; 2）幽门螺杆菌感染组; 3）碘缺乏组; 4）幽门螺杆菌+碘缺乏组; 5）5%NaCl组; 6）幽门螺杆菌+5%NaCl组; 7）5%NaCl+碘缺乏组; 8）幽门螺杆菌+5%NaCl+碘缺乏组。实验结束后处死动物（2005/4/4），HE染色：HP感染组（6组）胃底有明显炎性细胞浸润，HP感染组（2组）胃底有异位幽门腺，炎性细胞浸润严重。此外，在第2组和第3组的感染动物中，幽门粘膜的细胞增殖显著加速。第3组和第8组的感染动物显示幽门粘膜增生性变化、幽门腺囊性腺体扩张和糜烂。部分幽门腺多灶性穿透粘膜肌层，并延伸至粘膜下层。淋巴细胞、巨噬细胞和中性粒细胞浸润于前板和粘膜下层，并形成淋巴滤泡。另一方面，第1组动物中未观察到异常结果，而第3组动物中发现粘膜固有层严重损伤，伴有腺体缺损。另外，我们进行了阿辛蓝染色和PAS染色。