Analysis of apoptosis-related products in delayed neuronal death following cerebral ischemia

脑缺血后迟发性神经元死亡的凋亡相关产物分析

• 批准号: 10557129
• 负责人: TABUCHI Kazuo
• 金额: $ 4.67万
• 依托单位: Saga Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10557129/
• 关键词: cerebral ischemia; apoptosis; SELDI-TOF MS; midkine; Bcl-2; cerebrospinal fluid; 虚血; 神経細胞死; Bcl-xL; 遅発性神経細胞死; DNAfragmentation; TUNEL; Bax

We examined the internucleosomal DNA fragmentation by in situ DNA double breaks staining and immunohistochemical expression of Bcl-2 family gene products in rat hippocampal CA1 pyramidal neurons following transient ischemia (rat 4-ressels occlusion model). The degenerating dark neurons with condensed nuclei in CA1 were clearly stained for DNA strand breaks 2 days ischemia. Apoptotic indices which stand for percentage of positive stained nuclei in total nuclei were as follows ; O (Sham), 9.2 (Day 1), 68.2 (Day 2), 93.5 (Day4). Bcl-2α expression was observed in both CA1 and CA3 of sham-operated rats. However, positivity of Bcl-2 was decreated in CA1 and was increased in CA3 of ischemia rats, On the other hand, no staining of Bcl-xL in sham-operated group and positive staining in other group were demonstrated. No positive staining of Bax was observed in any groups. These results suggested that Bcl-2 gene products might be involved in apoptotic neuronal death in CA1 after transient ischemia.

通过原位DNA双断裂染色和Bcl-2家族基因产物的免疫组化表达检测大鼠海马CA1锥体神经元短暂性缺血后核体间DNA的断裂。缺血2 d后，CA1区退行性暗神经元的DNA链断裂明显染色。代表阳性染色细胞核占总细胞核百分比的凋亡指数如下：O(假),9.2(1天),68.2(2天),93.5(第4天)。假手术大鼠CA1和CA3中均有Bcl-2α的表达。而缺血大鼠CA1中Bcl-2的表达降低，CA3中Bcl-2的表达升高，假手术组未见Bcl-xL染色，其他组呈阳性。各组均未见Bax阳性染色。提示Bcl-2基因产物可能参与了CA1短暂性缺血后神经元凋亡的过程。

项目成果

Kawaguchi,S.: "Induction of apoptosis in glioma cells by recombinant human Fas ligand"Neurosurgery. 46. 431-439 (2000)

Kawaguchi,S.：“重组人 Fas 配体诱导神经胶质瘤细胞凋亡”神经外科。

Makoto Furuta, Tetsuya Shiraishi, Shigeo Ueda, Takashi Suzuyama, Kazuo Tabuchi, Yu Fukase Maoshi Arikuni: "Discovery of medulloblastoma biomarkers from cerebrospinal fluids using proteinchip SELDI-TOF Mass Spectrometry"Neuroimmunological Research. 13. 61-

Makoto Furuta、Tetsuya Shiraishi、Shigeo Ueda、Takashi Suzuyama、Kazuo Tabuchi、Yu Fukase Maoshi Arikuni：“使用蛋白质芯片 SELDI-TOF 质谱法从脑脊液中发现髓母细胞瘤生物标志物”神经免疫学研究。

田渕和雄,白石哲也,川口正二郎,一ノ瀬誠,増岡淳: "脳機能の解明"九州大学出版会. 8 (2000)

田渊和夫、白石哲也、川口正二郎、一之濑真、增冈润：“脑功能的阐明”九州大学出版社8（2000）。

古田誠,白石哲也,上田茂雄,鈴山堅志,田渕和雄,深瀬優,有國尚: "SELDT-TOF mass spectrometry(Protein Chip)を利用した髄芽腫の髄液バイオマーカー同定の試み"神経免疫研究. 13. 61-65 (2000)

Makoto Furuta、Tetsuya Shiraishi、Shigeo Ueda、Kenji Suzuyama、Kazuo Tabuchi、Masaru Fukase、Nao Arikuni：“尝试使用 SELDT-TOF 质谱（蛋白质芯片）识别脑脊液生物标志物”神经免疫学研究。 (2000)

古田誠,白石哲也,上田茂雄,鈴山堅志,田渕和雄,深瀬優,有國尚: "SELDI-TOF mass spectrometry(Protein Chip)を利用した髄芽腫の髄液バイオマーカー同定の試み"神経免疫研究. 13. 61-65 (2000)

Makoto Furuta、Tetsuya Shiraishi、Shigeo Ueda、Kenji Suzuyama、Kazuo Tabuchi、Yu Fukase、Nao Arikuni：“尝试使用 SELDI-TOF 质谱（蛋白质芯片）识别脑脊液生物标志物”神经免疫学研究。 (2000)