Analysis of apoptosis-related products in delayed neuronal death following cerebral ischemia

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We examined the internucleosomal DNA fragmentation by in situ DNA double breaks staining and immunohistochemical expression of Bcl-2 family gene products in rat hippocampal CA1 pyramidal neurons following transient ischemia (rat 4-ressels occlusion model). The degenerating dark neurons with condensed nuclei in CA1 were clearly stained for DNA strand breaks 2 days ischemia. Apoptotic indices which stand for percentage of positive stained nuclei in total nuclei were as follows ; O (Sham), 9.2 (Day 1), 68.2 (Day 2), 93.5 (Day4). Bcl-2α expression was observed in both CA1 and CA3 of sham-operated rats. However, positivity of Bcl-2 was decreated in CA1 and was increased in CA3 of ischemia rats, On the other hand, no staining of Bcl-xL in sham-operated group and positive staining in other group were demonstrated. No positive staining of Bax was observed in any groups. These results suggested that Bcl-2 gene products might be involved in apoptotic neuronal death in CA1 after transient ischemia.