Study on Oncogenes and Growth-Related Antigens of Human Brain Tumors
人脑肿瘤癌基因及生长相关抗原的研究
基本信息
- 批准号:01480551
- 负责人:
- 金额:$ 0.64万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (B)
- 财政年份:1989
- 资助国家:日本
- 起止时间:1989 至 1990
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Previous studies have demonstrated that the allelic deletions of the short arm of chromosome seventeenth (17p) and the long arm of the tenth (10q) are closely associated with the tumorigenesis of human malignant gliomas. Recent studies have shown that the cellular p53, which is encoded on chromosome17p13.1, may function as a suppressor of neoplastic growth and play an important role in the pathogenesis of human glial tumors. We have focused on the genetic abnormalities of p53 in relation to the development of human glial tumors, and investigated the structure and expression of p53 protein in glial tumors as well as the mutations of p53 gene. Our study demonstrated that several glioma cell lines had an aberrant form of p53 protein not only in its expression but also its molecular structure, and that p53 abnormalities might be an essential event associated with development of glial tumors. Immunoelectron microscopic study revealed that positive reaction for Ki-67 was on the granular and dense components of nucleolus, indicating that Ki-67 might participate in the processing and assembly of preribosmal particles. On the other hand, PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) analysis disclosed nucleotide substitution in 3 out of 7 human glial cell lines examined in exon 7 or 8 of the p53 gene, which were generally conserved over species. We assume these genetic alterations in coding sequence of the p53 gene are quite responsible mutations, because their products show prolonged half-life and are constantly expressed in the nuclei of human glial tumor cells. In view of these points, the genetic alterations of p53 gene appear to play a significant role in the development of glial cell neoplasia.
已有研究表明,第17号染色体短臂(17 p)和第10号染色体长臂(10 q)的等位基因缺失与恶性胶质瘤的发生密切相关。最近的研究表明,位于染色体17p13.1上的细胞p53可能作为肿瘤生长的抑制因子,在人类胶质瘤的发病机制中起重要作用。本研究主要探讨p53基因异常与胶质瘤发生发展的关系,并对p53蛋白在胶质瘤中的结构、表达及p53基因突变进行了研究。我们的研究表明,几个胶质瘤细胞系有一个异常的形式的p53蛋白不仅在其表达,而且其分子结构,和p53异常可能是一个重要的事件与胶质瘤的发展。免疫电镜观察显示,Ki-67阳性反应位于核仁的颗粒状和致密成分上,提示Ki-67可能参与了前核糖体颗粒的加工和组装。另一方面,PCR-SSCP(聚合酶链反应-单链构象多态性)分析显示,在p53基因的外显子7或8中检查的7个人神经胶质细胞系中有3个存在核苷酸取代,这些核苷酸取代通常在物种间保守。我们假设p53基因编码序列中的这些遗传改变是非常负责任的突变,因为它们的产物显示出延长的半衰期,并且在人类神经胶质肿瘤细胞的细胞核中不断表达。因此,p53基因的改变在胶质细胞瘤的发生发展中起重要作用。
项目成果
期刊论文数量(45)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
K. Tabuchi, T. Shiraishi, M. Takagi, N. Momozaki, T. Mineta: "Cell kinetic analysis of brain tumor" Brain tumor pathology. 6. 157-160 (1989)
K. Tabuchi、T. Shiraishi、M. Takagi、N. Momozaki、T. Mineta:“脑肿瘤的细胞动力学分析”脑肿瘤病理学。
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- 影响因子:0
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- 通讯作者:
K. Tabuchi: "Intracranial malignant melanoma" Clinical Neuroscience. 8. 366-367 (1990)
K. Tabuchi:“颅内恶性黑色素瘤”临床神经科学。
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- 影响因子:0
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田渕 和雄,他: "脳腫瘍の成長解析、Brdurd,Kiー67およびPCNA陽性率の比較" 脳腫瘍病理. 6. 157-160 (1989)
Kazuo Tabuchi 等:“脑肿瘤的生长分析,Brdurd、Ki-67 和 PCNA 阳性率的比较”《脑肿瘤病理学》6. 157-160 (1989)。
- DOI:
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- 影响因子:0
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田渕 和雄: "頭蓋内悪性黒色腫" Clinical Neuroscience. 8. 366-367 (1990)
Kazuo Tabuchi:“颅内恶性黑色素瘤”《临床神经科学》8. 366-367 (1990)。
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- 发表时间:
- 期刊:
- 影响因子:0
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田渕和雄: "脳腫瘍のoncogene" Clinical Neuroscience. 7. 70-73 (1989)
Kazuo Tabuchi:“脑肿瘤的癌基因”《临床神经科学》7. 70-73 (1989)。
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- 影响因子:0
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TABUCHI Kazuo其他文献
TABUCHI Kazuo的其他文献
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{{ truncateString('TABUCHI Kazuo', 18)}}的其他基金
Development of new strategies for cancer treatment based in system biological-analysis of cancer system abnormality
基于癌症系统异常的系统生物学分析开发癌症治疗新策略
- 批准号:
15390439 - 财政年份:2003
- 资助金额:
$ 0.64万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Biomarker Discovery of CSF biomarker of medulloblastoma by SELDI-TOF mass spectrometry
生物标志物 通过 SELDI-TOF 质谱法发现髓母细胞瘤的 CSF 生物标志物
- 批准号:
12470293 - 财政年份:2000
- 资助金额:
$ 0.64万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Analysis of apoptosis-related products in delayed neuronal death following cerebral ischemia
脑缺血后迟发性神经元死亡的凋亡相关产物分析
- 批准号:
10557129 - 财政年份:1998
- 资助金额:
$ 0.64万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
Molecular diagnosis of glioblastomas based on genetic alterations.
基于遗传改变的胶质母细胞瘤的分子诊断。
- 批准号:
09470298 - 财政年份:1997
- 资助金额:
$ 0.64万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
STUDY OF DNA FRAGMENTATION AND APOPTOSIS-RELATED GENE PRODUCTS IN HUMAN BRAIN TUMORS AND ESTABLISHMENT OF APOPTOSIS-INDUCING THERAPY.
人脑肿瘤中DNA片段和凋亡相关基因产物的研究及凋亡诱导疗法的建立。
- 批准号:
07457319 - 财政年份:1995
- 资助金额:
$ 0.64万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
STUDY ON THE DIAGNOSIS AND THERAPY OF BRAIN TUMORS IN TERMS OF GENETIC ENGINEERING
脑肿瘤的基因工程诊断和治疗研究
- 批准号:
04454363 - 财政年份:1992
- 资助金额:
$ 0.64万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Qualitative and quantitative study of oncogene-products in gliomas.
神经胶质瘤中癌基因产物的定性和定量研究。
- 批准号:
60570673 - 财政年份:1985
- 资助金额:
$ 0.64万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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