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Qualitative and quantitative study of oncogene-products in gliomas.

神经胶质瘤中癌基因产物的定性和定量研究。

基本信息

批准号：
60570673
负责人：
TABUCHI Kazuo
金额：
$ 0.83万
依托单位：
Saga Medical School (1986-1987)Kagawa Medical School (1985)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1987
项目状态：
已结题

项目摘要

Recent studies showed that the amino acid sequence of epidermal growth factor receptor(EGF-R) was strikingly similar to that of erb B transforming protein, the oncogene product of avian erythroblastosis virus(AEV). On the other hand, EGF-R and pp60^<src>, the product of src oncogene of Rous sarcoma virus(RSV) are believed to share the homologous amino acid sequence in their tyrosine kinase domains. To elucidate possible participation of certain oncogene in the development of glioma, four different human (KY, KH, KC & 118MG) glioma cell lines were examined for the presence of EGF-R,pp60^<src> and p21^<ras> by flow cytometry using specific monoclonal antibodies. It was found that all cell lines tested were positive for either EGF-R or pp60^<src>, or both, but none for p21^<ras>, though the degree of exzpression varied among cell lines. Furthermore, the expression of these oncogene-related products including EGF-R and pp60^<src> in glioma cells was markedly suppressed by such anti-glioma agents as prostaglandin(PG) D_2,J_2 and forskolin which could induce cell differentiation, whereas a very slight increased expression of EGF-R in KY cells was observed after the treatment with H-7, an inhibitor of protein kinase C. These results suggest that the src oncogene family including erb B might be involved in either the development and growth or differentiation of certain glial tumors.

最近的研究表明，表皮生长因子受体（epidermal growth factor receptor，EGF-R）的氨基酸序列与禽成红细胞增多症病毒（avian erythroblastosis virus，AEV）的癌基因产物er B B转化蛋白的氨基酸序列有着惊人的相似性。另一方面，认为EGF-R和<src>Rous肉瘤病毒（RSV）的src癌基因产物pp 60-α在它们的酪氨酸激酶结构域中共享同源氨基酸序列。为了阐明某些癌基因在胶质瘤发生中的可能作用，我们用特异性单克隆抗体通过流式细胞术检测了四种不同的人胶质瘤细胞系（KY、KH、KC和118 MG）中EGF-R、pp 60和p21的存在<src><ras>。结果发现，所有检测的细胞系都是EGF-R或pp 60 ~+阳性<src>，或两者都阳性，但没有p21 ~+阳性<ras>，尽管不同细胞系的表达程度不同。前列<src>腺素D_2，J_2和forskolin等抗胶质瘤药物可明显抑制胶质瘤细胞中EGF-R和pp 60 α的表达，而蛋白激酶C抑制剂H-7可使KY细胞中EGF-R的表达略有增加。这些结果提示src癌基因家族包括er B B可能参与某些胶质瘤的发生、生长或分化。