Qualitative and quantitative study of oncogene-products in gliomas.

最近的研究表明，表皮生长因子受体(EGF-R)的氨基酸序列与禽红母细胞增殖症病毒(AEV)癌基因产物erb B转化蛋白的氨基酸序列极为相似。另一方面，Rous肉瘤病毒(RSV)src癌基因产物EGF-R和pp60；；src&gt；被认为在其酪氨酸激酶区具有相同的氨基酸序列。为阐明某些癌基因在脑胶质瘤发生发展中的可能作用，我们用特异性的单抗，用流式细胞术检测了四种不同的人脑胶质瘤细胞系(KY、KH、KC和118 MG)中EGF-R、pp60和lt；src和gt；和p21和lt；ras和gt的表达。结果发现，所有细胞株都表达EGF-R或pp60；；src&gt；或两者均为阳性，但p21&lt；ras&gt；不同细胞系表达程度不同。此外，前列腺素(PG)D_2、J_2和Forsklin等可诱导细胞分化的抗胶质瘤药物可显著抑制胶质瘤细胞中EGF-R和pp60、lt、src和gt的表达，而蛋白激酶C抑制剂H-7处理后KY细胞中EGF-R的表达略有增加。这些结果提示，包括erb B在内的src癌基因家族可能参与了某些胶质瘤的发生、生长和分化。

Recent studies showed that the amino acid sequence of epidermal growth factor receptor(EGF-R) was strikingly similar to that of erb B transforming protein, the oncogene product of avian erythroblastosis virus(AEV). On the other hand, EGF-R and pp60^<src>, the product of src oncogene of Rous sarcoma virus(RSV) are believed to share the homologous amino acid sequence in their tyrosine kinase domains. To elucidate possible participation of certain oncogene in the development of glioma, four different human (KY, KH, KC & 118MG) glioma cell lines were examined for the presence of EGF-R,pp60^<src> and p21^<ras> by flow cytometry using specific monoclonal antibodies. It was found that all cell lines tested were positive for either EGF-R or pp60^<src>, or both, but none for p21^<ras>, though the degree of exzpression varied among cell lines. Furthermore, the expression of these oncogene-related products including EGF-R and pp60^<src> in glioma cells was markedly suppressed by such anti-glioma agents as prostaglandin(PG) D_2,J_2 and forskolin which could induce cell differentiation, whereas a very slight increased expression of EGF-R in KY cells was observed after the treatment with H-7, an inhibitor of protein kinase C. These results suggest that the src oncogene family including erb B might be involved in either the development and growth or differentiation of certain glial tumors.