Separation and Purification of Polyunsaturated Fatty acids by Chromatographic Process with Surface-treated Polymer Microspheres

表面处理聚合物微球色谱法分离纯化多不饱和脂肪酸

• 批准号: 11450300
• 负责人: KITAMURA Yoshiro
• 金额: $ 6.91万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11450300/
• 关键词: Polymeric Microparticle; Polymer Microsphere; Polyunsaturated Fatty Acids; Separation and Purification; Chromatographic Separation; Silver Ion; Ion Exchange; Adsorption

Poly (styrene-co-glycidyl methacrylate) (poly (St-GMA)) microspheres were prepared by the suspension polymerization, providing the polymer microspheres for separation of polyunsaturated fatty acids (PUFAs) such as docosahexaenoic acid (DHA) and eicosapentaacetic acid (EPA). These polymer microspheres had a potential to have ion-exchange property by ring-opening reaction of epoxy groups on the surface with disodium iminoacetate monohydrate. After the introduction of ion-exchange group onto polymer microspheres, Na^+ was exchanged with Ag^+ to prepare polymer microspheres bearing Ag^+ on their surface. It was well-known that π-electrons of carbon-carbon double bonds of polyunsaturated fatty acids were reacted with Ag^+ to form π-complexes, indicating that Ag^+ on the polymer microsphere surface have the affinity for polyunsaturated fatty acids. Adsorption equilibrium of Ag^+ on surface-treated polymer microspheres was well described with Langmuir isotherm. Adsorption experiments of ethyl ester of DHA (DHA-Et) were carried out with polymer microspheres having various amount of Ag^+. As a result, the amount of Ag^+ on polymer microspheres was found to be a significant determinant for adsorption of DHA-Et. The amount of docosahexaenoic acid adsorbed increased with an increase in amount of Ag^+ adsorbed.

采用悬浮聚合法制备了苯乙烯-甲基丙烯酸缩水甘油酯共聚物（St-GMA）微球，为分离二十二碳六烯酸（DHA）和二十碳五乙酸（EPA）等多不饱和脂肪酸（PUFA）提供了聚合物微球。这些聚合物微球具有潜在的离子交换性能，通过开环反应的环氧基团的表面与一水亚氨基乙酸二钠。在聚合物微球上引入离子交换基团后，通过Na^+与Ag^+的交换，制备了表面负载Ag^+的聚合物微球。多不饱和脂肪酸的碳碳双键上的π电子与Ag^+形成π络合物，表明聚合物微球表面的Ag^+对多不饱和脂肪酸具有亲合性。Ag^+在经表面处理的聚合物微球上的吸附符合Langmuir等温式。研究了不同Ag^+含量的聚合物微球对DHA乙酯（DHA-Et）的吸附性能。结果表明，聚合物微球上Ag^+的量是DHA-Et吸附的重要决定因素。随着Ag^+吸附量的增加，二十二碳六烯酸的吸附量也随之增加。