Separation and Purification of Polyunsaturated Fatty acids by Chromatographic Process with Surface-treated Polymer Microspheres

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Poly (styrene-co-glycidyl methacrylate) (poly (St-GMA)) microspheres were prepared by the suspension polymerization, providing the polymer microspheres for separation of polyunsaturated fatty acids (PUFAs) such as docosahexaenoic acid (DHA) and eicosapentaacetic acid (EPA). These polymer microspheres had a potential to have ion-exchange property by ring-opening reaction of epoxy groups on the surface with disodium iminoacetate monohydrate. After the introduction of ion-exchange group onto polymer microspheres, Na^+ was exchanged with Ag^+ to prepare polymer microspheres bearing Ag^+ on their surface. It was well-known that π-electrons of carbon-carbon double bonds of polyunsaturated fatty acids were reacted with Ag^+ to form π-complexes, indicating that Ag^+ on the polymer microsphere surface have the affinity for polyunsaturated fatty acids. Adsorption equilibrium of Ag^+ on surface-treated polymer microspheres was well described with Langmuir isotherm. Adsorption experiments of ethyl ester of DHA (DHA-Et) were carried out with polymer microspheres having various amount of Ag^+. As a result, the amount of Ag^+ on polymer microspheres was found to be a significant determinant for adsorption of DHA-Et. The amount of docosahexaenoic acid adsorbed increased with an increase in amount of Ag^+ adsorbed.