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Chromosome engineering for the development of transgenic avian

Chromosome engineering for the development of transgenic avian
用于开发转基因禽类的染色体工程
批准号:
11450313
负责人:
IIJIMA Shinji
金额:
$9.54万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B).
财政年份:
1999
资助国家:
日本
项目状态:
已结题
起止时间:
1999 至 2000

项目摘要

项目成果

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中文摘要
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英文摘要
We are trying to establish transgenic chicken by using a virus vector. For this, a pantropic retrovirus which is based on Moloney murine leukemia virus and contains VSV-G gene instead of env. This virus has several advantages such as broad host range. GFP and Neo^r gene. The former gene was expressed from virus LTR and the latter from inserted RSV promoters. A helper cell line which express virus gag and pot genes, was transfected with this vector and stable transfectants were isolated. VSV-G gene was transiently infected to one of the transfectant and replication-deficient virus particles were produced. By ultracentrifigation, virus particles were concentrated to 10^9 cfu/ml. Blastderm of quail was infected with this virus. After in vitro hatching, we analyzed the presence of vector DNA sequences. In almost all cells of Go chimera, this vector sequence could be detected.
期刊论文(4)
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会议论文
Shinji Mizuarai, Masamichi Kamihira, Ken-Ichi Nishijima and Shinji Iijima: "Integrase-mediated nonviral gene transfection with enhanced integration efficiency"Journal of Bioscience and Bioengineering. 88[5]. 461-467 (1999)
Shinji Mizuarai、Masamichi Kamihira、Ken-Ichi Nishijima 和 Shinji Iijima:“整合酶介导的非病毒基因转染,具有增强的整合效率”生物科学与生物工程杂志。
DOI: --
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影响因子: --
作者: []
通讯作者:
Yuichi Machida,Katsuhide Miyake,Kouji Hattori,Shin Yamamoto,Mitsuo Kawase and Shinji Iijima: "Structure and function of a novel coliphage-associated sialidase"FEMS Microbiology Letters. 182. 333-337 (2000)
Yuichi Machida、Katsuhide Miyake、Kouji Hattori、Shin Yamamoto、Mitsuo Kawase 和 Shinji Iijima:“新型大肠杆菌噬菌体相关唾液酸酶的结构和功能”FEMS 微生物学快报。
DOI: --
发表时间:
期刊:
影响因子: --
作者: []
通讯作者:
Shinji Mizuarai, Masamichi Kamihira, Ken-Ichi Nishijima and Shinji Iijima: "Integrase-mediated nonvital gene transfection with enhanced integration efficiency"Joumal of Bioscience and Bioengineering. 88[5]. 461-467 (1999)
Shinji Mizuarai、Masamichi Kamihira、Ken-Ichi Nishijima 和 Shinji Iijima:“整合酶介导的非生命基因转染,具有增强的整合效率”生物科学与生物工程杂志。
DOI: --
发表时间:
期刊:
影响因子: --
作者: []
通讯作者:
Construction of chicken mutant library using primordial germ cells
  • 批准号:
    25660291
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
  • 资助金额:
    $2.58万
  • 财政年份:
    2013
  • 负责人:
    IIJIMA Shinji
  • 依托单位:
The use of reproduction technology for the establishment of transgenic chicken
  • 批准号:
    18360393
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 资助金额:
    $11.35万
  • 财政年份:
    2006
  • 负责人:
    IIJIMA Shinji
  • 依托单位:
Improvement of Gene Therapy by Use of Aritificial Virus
  • 批准号:
    16360410
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 资助金额:
    $9.66万
  • 财政年份:
    2004
  • 负责人:
    IIJIMA Shinji
  • 依托单位:
Production of insulin in egg white by transgenic chicken
  • 批准号:
    13555225
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 资助金额:
    $8.77万
  • 财政年份:
    2001
  • 负责人:
    IIJIMA Shinji
  • 依托单位:
国内基金
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  • 项目类别:
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  • 资助金额:
    --
  • 批准年份:
    2026
  • 负责人:
    董娜
  • 依托单位:
SIRT1/PGC-1α信号轴调节线粒体葡萄糖代谢对人皮肤鳞状细胞癌作用机制研究
  • 批准号:
    2026JJ82418
  • 项目类别:
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  • 资助金额:
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  • 批准年份:
    2026
  • 负责人:
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