The investigation of chemiluminescence reaction in the micro reactor and its application to the detection in micro analytical system

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Three types of micro flow reactors with different length and width of channel were made by microfabrication techniques for the chemiluminescent (CL) detection of epinephrine (EP) with lucigenin. The volume of each CL reactor was about 10 μl. A solution containing EP and lucigenin and a solution containing NaOH and periodate were injected successively into each inlet of the CL reactor in the range from 20 to 100 μl min^<-1> with a pressure-driven flow system. The intensity of light emission was dependent on geometry of the CL reactors. These results could be explained on the basis of the difference in diffusion and mixing of the reactants in the CL reactors. The maximum light emission were linearly correlated with the concentrations of EP over the range from the detection limit of 5.0x10^<-8> M up to 5.0x10^<-6> M on the use of the CL reactor with the most promising geometry. In addition, we have investigated the enhanced CL reactions for applying to the micro analytical system.