Biomolecular Infrastructure for Detection of Radioisotopes, Fluorescence, Chemiluminescence

一台配备蓝色激光器的台风近红外生物分子成像仪和一台Azure 280凝胶成像仪是由发现补助金资助的申请人申请的，目的是支持约克大学生命科学大楼（LSB）的多个研究项目。约克大学的生命科学大楼支持26名主要研究人员，其中至少有15名来自NSERC发现赠款支持的生物学和化学系。这些研究计划严重依赖于检测和定量与感兴趣的核酸和蛋白质连接或相关的放射性同位素、荧光团和化学发光（ECL）水平的能力。 LSB中唯一用于凝胶和膜（即32 P标记的RNA和DNA或35 S标记的蛋白质）上的放射性同位素检测的仪器是2008年购买的Typhoon生物分子成像仪。该成像仪也是LSB中唯一能够检测蓝色波长荧光标记蛋白质或核酸的仪器。近日，2008年购买的Typhoon成像仪出现故障，厂家已表示不再支持，无法维修。这意味着目前不可能在LSB中进行定量的基于凝胶或膜的放射性同位素或荧光检测。ECL目前使用MicroChemi 2.0进行检测，该仪器的使用寿命即将结束，因为它现在经常出现错误，并且制造商不再提供部件或支持。因此，需要一套新的仪器，可用于放射性同位素，荧光和ECL检测是极其迫切的，因为依赖于这种功能的研究计划，他们的研究生产力受到严重阻碍。 LSB中有广泛的发现资助支持小组，研究各种研究课题，包括但不限于RNA加工，表观遗传学，信号转导，泛素和SUMO对蛋白质的翻译后修饰，宿主-微生物相互作用，细胞分化和细胞适应压力。所要求的仪器将是支持所有这些方案的关键基础设施。这些仪器将由研究生和其他高素质的人员使用，为学术界或工业界的未来职业生涯提供宝贵的培训。该申请将有利于申请人和学员从不同的范围寻求公平的群体。

A Typhoon NIR Biomolecular Imager with a Blue Laser and an Azure 280 Gel Imager are requested by Discovery Grant funded applicants for the purpose of supporting multiple research programs in the Life Science Building (LSB) at York University. York University's Life Science Building supports 26 primary investigators, including at least 15 in the Departments of Biology and Chemistry that are supported by NSERC Discovery Grants. These research programs rely heavily on the ability to detect and quantitate levels of radioisotopes, fluorophores and chemiluminescence (ECL) linked to or associated with nucleic acids and proteins of interest. The only instrument for radioisotope detection on gels and membranes (i.e. 32P labelled RNA and DNA, or 35S labelled proteins) in the LSB was a Typhoon Biomolecular Imager purchased in 2008. This imager was also the only instrument in the LSB that was capable of detecting blue wavelength fluorescently labelled proteins or nucleic acids. Recently, the Typhoon imager purchased in 2008 broke down, and the manufacturer has indicated that it is no longer supported and cannot be repaired. This means that it is currently impossible to perform quantitative gel or membrane-based radioisotope or fluorescence detection in the LSB. ECL is currently detected using a MicroChemi 2.0 that is at the end of its lifespan, as it is now routinely giving errors and for which parts or support is no longer provided by the manufacturer. Thus the need for a new set of instruments that can be used for radioisotope, fluorescence and ECL detection is extremely urgent, as research programs that rely on this functionality have had their research productivity severely hampered. There is a wide breadth of Discovery grant supported groups in the LSB studying diverse research topics including but not limited to RNA processing, epigenetics, signal transduction, post-translational modification of proteins by ubiquitin and SUMO, host-microbe interactions, cellular differentiation and cellular adaptations to stress. The requested instruments will represent critical infrastructure supporting all of these programs. The instruments will be used by graduate students and other highly qualified personnel, gaining a valuable training for future careers in academia or industry. The application will benefit applicants and trainees from a diverse breadth of equity seeking groups.