GENE EXPRESSION AND INTERNAL FEEDBACK REGULATION OF ETHYLENE BIOSYNTHESIS IN RIPENING FRUITS

成熟果实乙烯生物合成的基因表达和内部反馈调节

• 批准号: 11460013
• 负责人: INABA Akitsugu
• 金额: $ 9.86万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11460013/
• 关键词: FRUIT RIPENING; BANANA; TOMATO; ETHYLENE; ETHYLENE-RELATED GENES; ETHYLENE SIGNALING; ACC SYNTHASE; ACC OXIDASE; LE-FIL; アグロバクテリュウム; エチレン生合成; エチレン信号伝達; 遺伝子発現; ACC合成酵素; ガストランジェントアッセイ; LE-ACS6; MA-ACS1

Expression and internal feedback regulation of the genes related to ethylene biosynthesis during ripening in banana and tomato fruits were investigated.MA-ACS1 was the gene in banana fruit related to the ripening process and was inducible by exogenous ethylene. At the onset of climacteric, ethylene production increased greatly with a sharp peak concomitant with an increase in the accumulation of MA-ACS1 mRNA and then decreased rapidly. This sharp peak of ethylene production was accompanying a sharp peak of in vivo ACC oxidase activity. However, the level of MA-ACO1 transcripts remained high from the onset of climacteric to the fully ripe stage. This discrepancy between enzyme activity and gene expression of ACC oxidase was, at leastin part, due to reduced contents of ascorbate and ferrous, cofactors of the enzyme, during ripening.We examined extraction method for ACC synthase from banana pulp using polyethyleneglycol added buffer followed by acetone washing. The method gave a high ACC synthase activity in accordance with ethylene production throughout fruit ripening.A trasgenic tomato plants were obtained in which LE-EIL genes, a most down stream gene in ethylene signalings, were introduced into the plants.

研究了香蕉和番茄果实成熟过程中乙烯生物合成相关基因的表达及内反馈调节。MA-ACS1是香蕉果实成熟过程相关基因，可由外源乙烯诱导。在更年期开始时，乙烯产量大幅增加，并出现尖锐的峰值，伴随着 MA-ACS1 mRNA 积累的增加，然后迅速下降。乙烯产生的尖峰伴随着体内 ACC 氧化酶活性的尖峰。然而，从更年期开始到完全成熟阶段，MA-ACO1 转录物的水平仍然很高。 ACC 氧化酶的酶活性和基因表达之间的这种差异至少部分是由于成熟过程中酶的辅助因子抗坏血酸和亚铁的含量降低。我们研究了使用添加聚乙二醇的缓冲液然后丙酮洗涤从香蕉果肉中提取 ACC 合酶的方法。根据整个果实成熟过程中乙烯的产生，该方法给出了高ACC合酶活性。获得了转基因番茄植物，其中将乙烯信号传导最下游基因LE-EIL基因引入植物中。

项目成果

Lin, X.: "Extraction and properties of 1-aminocyclopropane-1-carboxylate synthase in banana fruit"Journal of the Japanese Society for Horticultural Science. 69-6. 696-701 (2000)

Lin, X.：“香蕉果实中 1-氨基环丙烷-1-羧酸合酶的提取和特性”日本园艺学会杂志。

Liu X., Y. Kubo, R. Nakano, and A. Inaba: "Extraction and properties of 1-aminocyclopropane-1-carboxylate synthase in banana fruit."Journal of the Japanese Society for Horticultural Science. 69-6. 696-701 (2000)

Liu X.、Y. Kubo、R. Nakano 和 A. Inaba：“香蕉果实中 1-氨基环丙烷-1-羧酸合酶的提取和特性。”日本园艺学会杂志。

Liu X., Shiomi S., Nakatsuka A, Kubo Y., Nakamura R and Inaba A.: "Characterization of ethylene biosynthesis associated with ripening in banana fruit."Plant Physiology. 121. 1257-1265 (1999)

Liu X.、Shiomi S.、Nakatsuka A、Kubo Y.、Nakamura R 和 Inaba A.：“与香蕉果实成熟相关的乙烯生物合成的特征。”植物生理学。

横谷尚起: "トマトのエチレン情報伝達系遺伝子EIN3ホモローグのクローニングと解析"園芸学会雑誌. 70・別2. 471 (2001)

Naoki Yokotani：“番茄乙烯信号基因EIN3同源物的克隆和分析”园艺学会杂志70，第2部分。471（2001）。

Lin, X.: "Characterization of ethylene biosynthesis associated with ripening in banana fruit"Plant Physiology. 121-4. 1257-1265 (1999)

Lin，X.：“与香蕉果实成熟相关的乙烯生物合成的特征”植物生理学。