MOLECULAR ENDOCRINOLOGICAL STUDY ON MECHANISMS OF ARTIFICIAL MATURATION IN FEMALE JAPANESE EEL

雌性日本鳗鱼人工成熟机制的分子内分泌学研究

• 批准号: 11460084
• 负责人: YAMAUCHI Kohei
• 金额: $ 5.95万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11460084/
• 关键词: JAPANESE EEL; PITUITARY; LIVER; OVARY; VITLLOGENIN; NATURAL EEL; GTH; STEROID HORMONE; 染色体; エストロゲンレセプター

1) Mechanism of pituitary GTH productionChanges in mRNA levels of GTH Iβ and IIβ subunits in pituitary of Japanese eel were examined during artificial maturation. The level of Iβ dramatically decreased immediately after the start of the treatment of artificial maturation, while the level of IIβdramatically increased. Experiment of pituitary organ culture revealed that estrogen and androgen strongly supressed the expression of Iβ, while they stimulated the expression of IIβ.2) Mechanism of ovarian sterod productioncDNAs of P450scc, P450c17, P450arom, 3β-HSD and 17β-HSD were cloned from Japanese eel ovary. The mRNA levels of ovarian P450scc, P450c17 , P450arom and 17β-HSD gradually increased during artificial maturation although the level of P450arom decreased in final maturation phase, while the level of 3β-HSD did not change.3) Mechanism of liver vitellogenin(VTG) productionThe mRNA levels of VTG1 in liver increased after mid-vitellogenic stage, while the level of VTG2 increased immediately after the start of the treatment of artificial maturation.4) Mechanism of sexual maturation in natural eelThe mRNA levels of GTH Iβ and IIβ in pituitary and various steroidogenic enzymes in ovary were compared between the artificially maturing Japanese eel (JP) and naturally maturing New Zealand longfinned eel (LF). The level of Iβ in LF was higher than that in JP. However, the levels of others were higher in JP than those in LF.

1)垂体促性腺激素生成机制研究了日本鳗鱼在人工成熟过程中脑垂体促性腺激素Iβ和IIβ亚基基因水平的变化。人工成熟处理后即刻I-β水平显著下降，II-β水平显著升高。垂体器官培养实验表明，雌激素和雄激素对Iβ的表达有强烈的抑制作用，而对IIβ的表达有促进作用。2)从日本鳗鱼卵巢中克隆了P450scc、P450c17、P450arom、3β-HSD和17β-HSD基因。卵巢P450scc、P450c17、P450arom和17β-HSDmRNA水平在人工成熟过程中逐渐升高，而在成熟末期P450arom的水平下降，而3β-HSD的水平没有变化。3)肝脏卵黄原蛋白的产生机制LF组I-β水平高于JP组。但JP组其他各指标水平均高于LF组。

项目成果

Kazeto: "Molecular cloning and characterization of Japanese eel ovarian P45Oc17 (CYP 17) cDNA"Gen. Comp. Endocrinol. 118. 123-133 (2000)

Kazeto：“日本鳗鱼卵巢 P45Oc17 (CYP 17) cDNA 的分子克隆和表征”Gen.

Ikeuchi: "Specific antibodies against synthetic oligopeptides of eel GTH/TSH subunits"Fisheries Sci.. 65. 871-877 (1999)

池内：“针对鳗鱼 GTH/TSH 亚基合成寡肽的特异性抗体”渔业科学 65. 871-877 (1999)

Kazeto: "Molecular cloning and characterization of Japanese eel ovarian P450c17 (CYP17) cDNA"Gen. Comp. Endocrinol.. 118. 123-133 (2000)

Kazeto：“日本鳗鱼卵巢 P450c17 (CYP17) cDNA 的分子克隆和表征”Gen.

Ozaki: "Developmental changes in pituitary-thyroid axis, and formation of gonads in leptocephali and glass eels of Anguilla spp."Fishereis Sci.. 66. 1115-1122 (2000)

尾崎：“垂体-甲状腺轴的发育变化以及安圭拉鳗鱼和玻璃鳗中性腺的形成。”Fishereis Sci.. 66. 1115-1122 (2000)

Kayaba: "Ultrastructure of the oocytes of the Japanese eel Anguilla japonica during artificially induced sexual maturation"Fisheries Sci.. 67. 870-879 (2001)

Kayaba：“人工诱导性成熟过程中日本鳗鲡卵母细胞的超微结构”渔业科学 67. 870-879 (2001)