Production of goat lines selected by genes of MHC class II and challenge for improve to disease resistance

(1) MHCⅱ类固定等位基因山羊系的选育。首席研究员天野博士于1986年3月从韩国引进了3只本地山羊（1公2母）到东京农业大学动物育种遗传实验室。利用这些家畜，我们已经根据MHC II类DRB3的三种不同基因生产了三个山羊系。为了明确3个山羊品系的遗传变异，对27个基因座编码的血蛋白进行了分析，发现其中PA-3和Alp 2个基因座存在多态性。多态性位点比例（P poly）在0.074 ~ 0.037之间，平均杂合度(H)在0.0049 ~ 0.0239之间。P多聚值和H值表明不同品系山羊的遗传变异水平不同。为了明确鉴定并进行亲子鉴定，我们还检测了50个微卫星标记。其中一个标记是MHCII-DR标记，它根据山羊的线来分类。等位基因数量在1 ~ 4之间。86%的位点被检测为多态性。从这些分析中发现，这50个微卫星标记对我们的山羊是有用的。标记杂合度范围在0.245 ~ 0.292之间，与日本沙羊的0.535范围相比处于较低水平，与西巴山羊的杂合度范围相同。利用该品系的4 ~ 5个多重标记，可以对该品系的山羊进行亲子鉴定。2)葡萄球菌超抗原刺激MHCⅱ类单倍型固定山羊的免疫应答本研究旨在了解葡萄球菌超抗原（sAg）刺激MHCⅱ类单倍型固定山羊的免疫应答。为此，我们拟建立山羊乳腺免疫系统免疫模型。我们专注于体外和体内研究；外周血T细胞和乳腺T细胞对sAg的反应性表征，以及T细胞受体Vb与sAg结合的鉴定。采用3株不同MHCⅱ类单倍型的山羊菌株A、D和E，探讨sag驱动的T细胞应答。然而，在MHC II类单倍型不同山羊之间没有观察到显著水平的vb依赖性T细胞反应。目前的研究表明，这些山羊菌株为通过细菌感染应激探索免疫和致病事件提供了强有力的反刍动物模型。少

(1) The breeding of the goat lines with fixed allele of MHC class IIThe head investigator Dr. Amano imported three individuals of native goats, that is one male and two Females, from Korea to the Laboratory of Animal Breeding and Genetics, Tokyo University of Agriculture, in march 1986. Using this stock animals, we have been produced three lines of goats based on three varied genes, of MHC class II DRB3. To clarify the genetic variations of the animals of three goat lines, blood proteins which were encoded by 27 genetic loci were analyzed, and 2 of them, PA-3 and Alp, were found to be polymorphic. The values of proportion of polymorphic loci (P poly) ranged between 0.074 and 0.037, and the average heterozygosity (H) ranged between 0.0049 and 0.0239. The values of P poly and H suggest that the level of genetic variavilities are different by lines of goat. To clarify the identification and to carry out the parentage test, we also tested 50 microsatellite markers. One of the markers, that … More is MHCII-DR marker classified goats by its lines. The number of alleles ranged from 1 to 4. Eighty-six per cent of loci were detected as polymorphic. From these analyses it was found that these 50 microsatellite markers are useful for our goats. Marker heterozygosity ranged between 0.245 to 0.292, this value showed low level by comparison with he range of 0.535 in Japanese Saanen, and same level as the range of Siba goat. It was possible to carry out the parentage testing in goats of the lines by using 4 to 5 multiple markers of them.2) Immune response on MHC class II haplotype fixed goat stimulated by the Staphylococcus superantigenThis study is to understand the immune response on MHC class II haplotype fixed goat stimulated by the Staphylococcus superantigen (sAg). For this object, we plane to establish a goat model, which can be broke up the mammary immune system by sAg. We focused on following in vitro and in vivo studies ; characterization of reactivity of peripheral blood T cells and mammary gland T cells to sAgs, and identification of T cell receptor Vb bind with sAg. Three goat strains which were have different MHC class II haplotype, A, D and E, were employed to explore sAg-driven T cells responses. However, no significant levels of Vb-dependent T cell responses were observed between MHC class II haplotype different goat. In the present studies indicated that these goat strains have proven powerful ruminant model for exploring immunological and pathogenic event in through the bacteria infectious stress. Less