Production of goat lines selected by genes of MHC class II and challenge for improve to disease resistance

MHC II类基因选择的山羊品系的生产及提高抗病性的挑战

• 批准号: 11460131
• 负责人: AMANO Takashi
• 金额: $ 9.02万
• 依托单位: Tokyo University of Agriculture
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11460131/
• 关键词: goat; closed population; breeding of experimental animal line; MHC class II; superantigen; mastitis; microsatellite marker; blood protein polymorphism; 寒地適応; 免疫応答

(1) The breeding of the goat lines with fixed allele of MHC class IIThe head investigator Dr. Amano imported three individuals of native goats, that is one male and two Females, from Korea to the Laboratory of Animal Breeding and Genetics, Tokyo University of Agriculture, in march 1986. Using this stock animals, we have been produced three lines of goats based on three varied genes, of MHC class II DRB3. To clarify the genetic variations of the animals of three goat lines, blood proteins which were encoded by 27 genetic loci were analyzed, and 2 of them, PA-3 and Alp, were found to be polymorphic. The values of proportion of polymorphic loci (P poly) ranged between 0.074 and 0.037, and the average heterozygosity (H) ranged between 0.0049 and 0.0239. The values of P poly and H suggest that the level of genetic variavilities are different by lines of goat. To clarify the identification and to carry out the parentage test, we also tested 50 microsatellite markers. One of the markers, that … More is MHCII-DR marker classified goats by its lines. The number of alleles ranged from 1 to 4. Eighty-six per cent of loci were detected as polymorphic. From these analyses it was found that these 50 microsatellite markers are useful for our goats. Marker heterozygosity ranged between 0.245 to 0.292, this value showed low level by comparison with he range of 0.535 in Japanese Saanen, and same level as the range of Siba goat. It was possible to carry out the parentage testing in goats of the lines by using 4 to 5 multiple markers of them.2) Immune response on MHC class II haplotype fixed goat stimulated by the Staphylococcus superantigenThis study is to understand the immune response on MHC class II haplotype fixed goat stimulated by the Staphylococcus superantigen (sAg). For this object, we plane to establish a goat model, which can be broke up the mammary immune system by sAg. We focused on following in vitro and in vivo studies ; characterization of reactivity of peripheral blood T cells and mammary gland T cells to sAgs, and identification of T cell receptor Vb bind with sAg. Three goat strains which were have different MHC class II haplotype, A, D and E, were employed to explore sAg-driven T cells responses. However, no significant levels of Vb-dependent T cell responses were observed between MHC class II haplotype different goat. In the present studies indicated that these goat strains have proven powerful ruminant model for exploring immunological and pathogenic event in through the bacteria infectious stress. Less

(1)MHC类固定等位基因山羊品系的选育。1986年3月，天野之弥博士从韩国引进了三只本地山羊，即一公两母，用于东京农业大学动物育种与遗传学实验室。利用这些家畜，我们已经培育了基于三个不同基因的三个山羊品系，MHC II类DRB3。为了弄清三个山羊品系动物的遗传变异，对27个遗传座位编码的血液蛋白进行了分析，发现PA-3和ALP两个基因座具有多态性。多态座位比例(P Poly)在0.074~0.037之间，平均杂合度(H)在0.0049~0.0239之间。P、Poly值和H值表明，不同品系山羊的遗传变异水平不同。为了明确鉴定和进行亲子鉴定，我们还检测了50个微卫星标记。其中一个标志，就是…更多的是MHCII-DR标记按其品系对山羊进行分类。等位基因数目从1个到4个不等，86%的座位具有多态。从这些分析中发现，这50个微卫星标记对我国山羊是有用的。标记杂合度在0.245~0.292之间，与日本萨能羊0.535的范围相比，表现出较低的水平，与锡巴山羊的范围相同。2)葡萄球菌超抗原对MHC-II单倍型固定山羊的免疫应答本研究旨在了解葡萄球菌超抗原对MHC-II单倍型固定山羊的免疫应答。为此，我们计划建立一种山羊模型，该模型可以用SAG来破坏乳腺免疫系统。我们主要进行了以下几个方面的体内外研究：外周血T细胞和乳腺T细胞对SAG的反应性鉴定，以及与SAG结合的T细胞受体Vb的鉴定。以三个具有不同MHC II类单倍型的山羊品系A、D和E为研究对象，探讨SAG诱导的T细胞应答。然而，在MHC-II单倍型不同山羊之间，没有观察到依赖VB的T细胞反应的显著水平。目前的研究表明，这些山羊品系已被证明是通过细菌感染应激来探索免疫学和致病事件的强大的反刍动物模型。较少

项目成果

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D.Nyamsamba, K.Nomura, K.Nozawa, M.Yokohama, Yo.Zagdsuren, T.Amano: "Genetic relationship among Mongolian native goat populations estimated by blood protein polymorphism"Small ruminant research. 47. 171-181 (2003)

D.Nyamsamba、K.Nomura、K.Nozawa、M.Yokohama、Yo.Zagdsuren、T.Amano：“通过血液蛋白多态性估计蒙古本土山羊种群之间的遗传关系”小反刍动物研究。

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S.Ogawa, G.Nagamatsu, M.Watanabe, S.Watanabe, T.Hayashi, R.Abe et al.: "Opposing effects of anti-activation-inducible lymphocyte-immunomodulatory molecule/inducible constimulator antibody on the development of acute versus"J. Immunol.. 15. 5741-5748 (2001

S.Okawa、G.Nagamatsu、M.Watanabe、S.Watanabe、T.Hayashi、R.Abe 等人：“抗活化诱导淋巴细胞免疫调节分子/诱导刺激抗体对急性与