BASIC STUDIES ON THE PRODUCTION AND UTILIZATION OF MONOCLONAL ANTIBODIES IN ANIMAL HUSBANDRY FIELD

单克隆抗体生产及畜牧业应用的基础研究

• 批准号: 02660286
• 负责人: AMANO Takashi
• 金额: $ 1.28万
• 依托单位: FACULTY OF AGRICULTURE, TOKYO UNIVERSITY OF AGRICULTURE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02660286/
• 关键词: Monoclonal antibody; Blood groups; Cattle; Water buffalo; Horse; Goat; Red cell antigen; 共通性抗原; モノクロ-ナル抗体; 牛血液型; 水牛血液型; 馬血液型; 肉種鑑別; 抗ミオグロビン

Main research object is the basic studies on production and utilization of monoclonal anti-bodies for animal blood group antigens. The research results are summarized as follows.1. The 38 kinds of monoclonal antibodies reacting with A, B, C, F, S, Z systems in cattle, 8 kinds of antibodies reacting with A, C, P systems in horses, 13 kinds of antibodies in water buffaloes and 9 kinds of antibodies in goats were established.2. Among these monoclonal antibodies, some antibodies recognized subtype antigens belonging the same blood group system showed quantitative reaction, namely monoclonal antibody identified each subtype antigen by the antibody titers, that is A_1/A_2 of A system, F_1/Fc of F system, S_1/S_2, U_1/U_2/U_3(SU) of S system in cattle, Ac/Aa, Ac/Aa of A system、 Pc/Pa of P system in horses, NW-2/NW-8/NW-3 in water buffaloes. The results indicated that the quantitative relationships exist between subtype antigens.3. Antigens, that is A_2, H of A system, Fc of F system、 some of S system, existed also in water buffaloes and goats, and X_2 antigen existed in water buffaloes as common antigens with cattle.4. The possibility to establish the monoclonal antibodies for the identification of meat species was obtained by using horse mioglobin.

主要研究对象为动物血型抗原单克隆抗体的制备和应用的基础研究。主要研究成果如下：1.建立了38种与牛A、B、C、F、S、Z系统反应的单克隆抗体，8种与马A、C、P系统反应的单克隆抗体，13种与水牛反应的单克隆抗体，9种与山羊反应的单克隆抗体.在这些单克隆抗体中，识别同一血型系统亚型抗原的抗体有定量反应，即单克隆抗体通过抗体滴度识别各亚型抗原，即牛A系统的A_1/A_2，F系统的F_1/Fc，S系统的S_1/S_2、U_1/U_2/U_3（SU），马A系统的Ac/Aa、Ac/Aa，P系统的Pc/Pa，NW-2/NW-8/NW-3在水牛中。结果表明，各亚型抗原之间存在定量关系.水牛和山羊中也存在抗原，即A系统的A_2、H、F系统的Fc、部分S系统的抗原，水牛中存在X_2抗原，作为与牛的共同抗原。4.利用马肌红蛋白建立单克隆抗体用于肉类品种鉴定的可能性。

项目成果

NOMURA,Koh: "The production and characterization of monoclonal antibodies to bovine blood group antigens" Animal Science and Technology(Jpn.). 62. 336-342 (1991)

NOMURA，Koh：“牛血型抗原单克隆抗体的生产和表征”动物科学与技术（日本）。

Nomura, k: "The production and characterization of monoclonal antibodies to bovine blood group antigens" Anim. Sci. Technol. (Jpn.). 62(4) : 62(4). 336-342 (1991)

Nomura, k：“牛血型抗原单克隆抗体的生产和表征”Anim。

NOMURA, Koh: "The Production and Characterization of Monoclonal Antibodies to Bovine Blood Group Antigens" Animal Science and Technology. 62. 336-342 (1991)

NOMURA, Koh：“牛血型抗原单克隆抗体的生产和表征”动物科学与技术。