Construction of detection system for prion protein using recombinant chicken monoclonal antibody

重组鸡单克隆抗体朊病毒蛋白检测体系的构建

• 批准号: 11460138
• 负责人: MATSUDA Haruo
• 金额: $ 4.22万
• 依托单位: Hirosima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11460138/
• 关键词: Chicken; Monoclonal antibody; prion disease; phage displayed antibody

The chicken is a useful animal for the development of the specific antibodies against the mammalian conserved proteins. In this study, we generated construction of expression vector for chicken single chain fragment of variable region (scFv), named as pCPDS, and two types of chicken scFv antibodies specific for prion protein. In the Western blotting and ELISA analysis, the recombinant monoclonal antibodies were reactive to prion protein from mouse brains. In the foundamental examination for immuno-PCR using phage displayed chicken monoclonal antibody, furthermore, these antibodies were detectable to prion protein peptide with high specificity.

鸡是开发针对哺乳动物保守蛋白的特异性抗体的有用动物。本研究构建了鸡源单链抗体可变区（scFv）的表达载体pCPDS，并制备了两种鸡源特异性抗朊蛋白的单链抗体。Western blotting和ELISA分析表明，重组单克隆抗体与小鼠脑朊蛋白呈阳性反应。用噬菌体展示的鸡单克隆抗体进行免疫PCR的基础试验，发现这些抗体对朊病毒蛋白肽段具有高度的特异性。

项目成果

Nakamura, N., Horiuchi, H., Furusawa, S., and Matsuda, H.: "Construction of recombinant chicken monoclonal antibody using phage displayed system"Curr. Prog, on Avian Immunol. Res. Group. 335-340 (2000)

Nakamura, N.、Horiuchi, H.、Furusawa, S. 和 Matsuda, H.：“使用噬菌体展示系统构建重组鸡单克隆抗体”Curr。

Yukko OTSUBO ほか: "Role of bursin on the development of B lymphocytes in chicken embryonic bursa of Fabricius."Dev.Comp.Immunol.. (in press). (2001)

Yukko OTSUBO 等人：“法氏囊鸡胚胎法氏囊中法氏囊 B 淋巴细胞发育的作用”。Dev.Comp.Immunol..（出版中）。

Komei FUKUSHIMA ほか: "In vitro system for immunoglobulin class switching using BHK cells transfected with murine recombinant CD4O ligand"Journal of Veterinary Medical Science. 61. 615-619 (1999)

Komei FUKUSHIMA 等人：“使用鼠重组 CD4O 配体转染的 BHK 细胞进行免疫球蛋白类别转换的体外系统”《兽医医学科学》杂志 61. 615-619 (1999)。

Nakamura, N., Aoki, Y., Horiuchi, H., Furusawa, S., Yamanaka, H., Kitamoto, T., and Matsuda, H.: "Construction of recombinant monoclonal antibodies from a chicken hybridoma line secreting specific antibody"Cytotechnol.. 31. 1-8 (2000)

Nakamura, N.、Aoki, Y.、Horiuchi, H.、Furusawa, S.、Yamanaka, H.、Kitamoto, T. 和 Matsuda, H.：“从分泌特异性抗体的鸡杂交瘤系中构建重组单克隆抗体

N.NAKAMURAほか: "Construction of recombinant chicken monoclonal antibody using phage displayed system"Current Progress on Avian Immunology Research. 335-340 (2000)

N.NAKAMURA 等：“使用噬菌体展示系统构建重组鸡单克隆抗体”《禽类免疫学研究进展》335-340 (2000)。