Human Liver Cell and Pancreatic Cell Transplantation-Study Using Resected Liver and Pancreas Segment-

肝细胞或胰岛细胞移植已被研究作为肝或胰腺移植的替代方案，用于治疗肝代谢紊乱或重症胰腺炎和糖尿病患者。肝细胞移植治疗急慢性肝功能衰竭正从实验室走向临床。目前全世界已有80多例肝细胞移植，其中一个主要的限制因素是高质量细胞的可获得性，因为人肝细胞来源于切除的移植物。还需要评估肝再生的机制，这将改善移植的人肝细胞的存活和功能。这种方法可能使肝细胞移植在不久的将来成为肝移植的一种可接受和实用的替代方法。为了实现这一过程，必须建立能够提供大量肝细胞的肝细胞库系统。我们曾报道一种简便的冷冻保存肝癌的方法 关于我们 使用微胶囊化技术测试。在这里，我们研究了如何在冷冻保存过程中避免微囊化肝细胞的冷冻损伤。低温显微镜显示，海藻酸钠微囊化技术保护肝细胞免受细胞外冰晶生长引起的物理损伤。超微结构检查显示微囊化肝细胞的细胞内环境得以维持。我们的微囊化技术保护肝细胞免受冷冻损伤。部分肝切除术后肝再生的调控是一个复杂的过程，涉及到许多不同的细胞因子。我们研究了其中之一，转化生长因子-β 1（TGF-β 1），肝再生抑制剂的作用。处理动物的肝脏中S期细胞数量增加。PHx后24 h抗体给药导致增殖的最高峰;此外，在那时也观察到峰值MIB-5标记。然而，任何动物之间的残留肝脏重量/体重比和再生率均无显著差异。这些结果表明，TGF-β 1可能抑制G1检查点，血清TGF-β 1浓度可能影响HGF调节PHx后肝再生和维持增殖的稳态。这些研究将在不久的将来促进肝细胞或胰腺细胞移植。少

Hepatocyte or pancreatic islets cell transplantations have investigating as an alternative to liver or pancreas transplantation in patients with liver-based metabolic disorders or with severe pancreatitis and diabetes mellitus. Hepatocyte transplantation is making its transition from bench to bedside for acute and chronic liver failure. More than 80 patients have now been transplanted world wide.A major limiting factor is the availability of good quality cells as human hepatocytes are derived from resected grafts. There is also a need to evaluate the mechanism of liver regeneration that will improve the survival and function of transplanted human hepatocytes. Such approach may allow hepatocyte transplantation to become an accepted and practical alternative to liver transplantation in the near future. To realize this procedure, a hepatocyte bank system capable of supplying large numbers of hepatocytes must be established. We previously reported an easy method for cryopreserving hepatocy … More tes using a microencapsulation technique. Here, we investigated how cryoinjury to microencapsulated hepatocytes could be avoided during cryopreservation. Cryomicroscopy showed that the alginate microencapsulation technique protected the hepatocytes from physical damage caused by the growth of extracellular ice crystals. Ultrastructural examination revealed that the intracellular environment of the microencapsulated hepatocytes was maintained. Our microencapsulation technique protects hepatocytes from cryoinjury. This novel technique could be utilized by hepatocyte banks.The regulation of liver regeneration after partial hepatectomy (PHx) is complex and involves many different cytokines. We investigated the role of one of these, transforming growth factor-beta1 (TGF-beta1), an inhibitor of liver regeneration. Livers from treated animals contained an increased number of cells in S phase. Antibody administration 24 h after PHx resulted in the highest peak of proliferation ; moreover, peak MIB-5 labeling was also observed at that time. However, neither residual liver-weight-to-body-weight ratios nor regeneration rates differed significantly between any of the animals. These results indicate that TGF-beta1 may inhibit the G1 checkpoint, and serum TGF-beta1 concentration may influence HGF to regulate liver regeneration and to maintain homeostasis of proliferation after PHx. These investigations willl promote hepatocyte or pancreatic cell transplantaion in near future. Less