Evaluation of Malignant Potential in Gastroenterological Tumors Using by CGH

我们用比较基因组杂交(CGH)和荧光原位杂交(FISH)研究了结直肠癌。对已知c-myc基因扩增的SRM细胞系进行CGH分析，发现在SRM中，3q、8q、11q扩增，8p2缺失。在对12例结直肠癌的调查中，发现20p(6/12)和20q(7/12)扩增，18q(8/12)和22q(5/12)缺失。有肝转移的结直肠癌约占10%，与预后有关。采用比较基因组杂交(CGH)技术，对9例结肠癌肝转移患者和19例无肝转移患者的染色体异常进行了分析。肝转移8q(5/9)、20p(5/9)、20q(8/9)的结直肠癌染色体均有增加。在无肝转移的结直肠癌中，可观察到染色体8q(2/19)、20p(6/L9)、20q(5/19)的增加。在FISH中，7、17、18和20个拷贝数增加，特别是20个拷贝数增加了84.6%。因此，CGH和FISH是分析结直肠癌遗传变化的有用方法。在CGH中，有肝转移的结直肠癌与20Q的扩增有关。

We have investigated colorectal cancers by Comparative Genomic Hybridizaton(CGH) and Fluorescence in situ hybridization(FISH).1. We investigated the cell line of SRM that was known the amplification of c-myc previously, by CGH.In SRM, it recognized the amplification in 3q, 8q and 11q and the loss in 8p.2. In the investigation in 12 cases in colorectal cancer, it recognized the amplification in 20p(6/12) and 20q(7/12) and the loss in 18q(8/12) and 22q(5/12).3. A colorectal cancer with liver metastasis appears about 10% and relates to the prognosis. We investigate a genetic changes of a colorectal cancer with liver metastasis by Comparative Genomic Hybridization(CGH) In this study, we analyzed the abnormal chromosomes in nine cases with colon cancer with liver metastasis and 19 cases without liver metastasis. A gain in chromosomes in colorectal cancers with liver metastasis of 8q(5/9), 20p(5/9), 20q(8/9) was observed. In colorectal cancers without liver metastasis gains in chromosomes of 8q(2/19), 20p(6/l9), 20q(5/19) were observed.4. In FISH, the gain in a number of copy in 7, 17, 18 and 20. Especially, the gain in a number of copy of 20 showed 84.6%.Therefor, CGH and FISH are the useful analysis in a genetic changes of a colorectal cancer. In CGH, a colorectal cancer with liver metastasis is concerned with the amplification in 20q.