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Evaluation of Malignant Potential in Gastroenterological Tumors Using by CGH

使用 CGH 评估胃肠道肿瘤的恶性潜能

基本信息

批准号：
08457332
负责人：
KUSANO Mitsuo
金额：
$ 3.01万
依托单位：
Showa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457332/
关键词：
CGH FISH Colorectal cancer Liver metastasis Flow Cytometry DNA 染色体異常染色体の増幅・欠失

项目摘要

We have investigated colorectal cancers by Comparative Genomic Hybridizaton(CGH) and Fluorescence in situ hybridization(FISH).1. We investigated the cell line of SRM that was known the amplification of c-myc previously, by CGH.In SRM, it recognized the amplification in 3q, 8q and 11q and the loss in 8p.2. In the investigation in 12 cases in colorectal cancer, it recognized the amplification in 20p(6/12) and 20q(7/12) and the loss in 18q(8/12) and 22q(5/12).3. A colorectal cancer with liver metastasis appears about 10% and relates to the prognosis. We investigate a genetic changes of a colorectal cancer with liver metastasis by Comparative Genomic Hybridization(CGH) In this study, we analyzed the abnormal chromosomes in nine cases with colon cancer with liver metastasis and 19 cases without liver metastasis. A gain in chromosomes in colorectal cancers with liver metastasis of 8q(5/9), 20p(5/9), 20q(8/9) was observed. In colorectal cancers without liver metastasis gains in chromosomes of 8q(2/19), 20p(6/l9), 20q(5/19) were observed.4. In FISH, the gain in a number of copy in 7, 17, 18 and 20. Especially, the gain in a number of copy of 20 showed 84.6%.Therefor, CGH and FISH are the useful analysis in a genetic changes of a colorectal cancer. In CGH, a colorectal cancer with liver metastasis is concerned with the amplification in 20q.

我们用比较基因组杂交（CGH）和荧光原位杂交（FISH）研究了结直肠癌。我们研究了以前已知的通过CGH扩增c-myc的SRM细胞系。在SRM中，它识别出3q、8q和11q的扩增和8p.2的缺失。在对12例结直肠癌患者的调查中，发现20p（6/12）和20q（7/12）基因扩增，18q（8/12）和22q（5/12）基因缺失。结直肠癌伴肝转移的发生率约为10%，并与预后有关。本研究采用比较基因组杂交技术（CGH）对9例结直肠癌伴肝转移和19例无肝转移患者的异常染色体进行了分析。结直肠癌肝转移患者的染色体增加8q（5/9）、20p（5/9）、20q（8/9）。在未发生肝转移的结直肠癌患者中，8q（2/19）、20p（6/ 19）、20q（5/19）染色体均有增加。在FISH中，7、17、18和20的拷贝数的增益。特别是20个副本的数量增加了84.6%。因此，CGH和FISH是分析结直肠癌基因变化的有效方法。在CGH中，结直肠癌伴肝转移与20q的扩增有关。