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Basic research of the memory fixation facilitation by the anesthetics - Multipoint analysis of hippocampus LTP and Ca imaging -

麻醉剂促进记忆固定的基础研究 - 海马 LTP 和 Ca 成像的多点分析 -

基本信息

批准号：
11470324
负责人：
UEKI Masaaki
金额：
$ 6.53万
依托单位：
Kagawa Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

MED64SYSTEM (Matsushita Electric Industrial) was used in respect of 64 channel multipoint extacellular stimulation/record in mouse and rat brain slice. By having constant temperature layer for noise counterplan and thermal control, the experiment was carried out in respect of the whole chamber.1. Mouse/rat brain slide preparation and multipoint recording of evoked potential.The slice of thickness 350 microns was made under the ether anesthesia in respect of the mouse in decapitation and after the cooling. As a problem in the preceding fiscal year, though the peeling in the slice piece under reflux is considerably improved, it comes in, and it is yet insufficient, and will continue in future too and will be devised.2. The formation of the long-term potentiation.15-20 By adding tetanic stimulation after the control stimulation in the minute, the long-term potentiation was induced. The phenomenon in which evoked potential of the control gradually increased was often observed. For this, it is necessary to examine incubation condition (temperature and time), and stimulation condition (intensity and interval of the pulse) of the slice further.3. Mouse/rat brain slice preparation and patch clamp, high-speed calcium imaging synchronization measurement.The back is guessed at present whole cell patch yet at last would synaptic transmission LTP anesthesia post-increase presynapse, and the high-speed calcium imaging will be carried out.

采用MED64SYSTEM （Matsushita Electric Industrial）对小鼠和大鼠进行64通道多点细胞外刺激/脑切片记录。通过设置恒温层进行噪声对抗和热控制，对整个腔室进行了实验。小鼠/大鼠脑切片制备及诱发电位多点记录。分别在乙醚麻醉下对小鼠断头和冷却后切片，切片厚度为350微米。1 .作为上一会计年度的问题，回流下切片的剥皮虽然有了很大的改善，但还是进来了，还不够，以后还会继续，还会设计。长期增强的形成。15-20在对照刺激后，于分钟内加强直性刺激，诱导长时程增强。经常观察到对照组的诱发电位逐渐升高的现象。为此，有必要进一步考察切片的孵育条件（温度和时间）和刺激条件（脉冲强度和间隔）。小鼠/大鼠脑切片制备和膜片钳，高速钙成像同步测量。目前推测背部全细胞贴片，最后将突触传递LTP麻醉后增加突触前，并进行高速钙显像。