Basic research of the memory fixation facilitation by the anesthetics - Multipoint analysis of hippocampus LTP and Ca imaging -

采用MED64SYSTEM(松下电工)对小鼠和大鼠脑片进行经络多点细胞外刺激/记录。通过设置恒温层用于噪声抑制和热控制，对整个燃烧室进行了实验。小鼠/大鼠脑切片制备及诱发电位多点记录，在乙醚麻醉下对小鼠断头及降温后制作350微米厚的脑片。作为上一财年的一个问题，虽然回流时切片的剥落有了很大的改善，但它来了，还不够，将来也会继续，并将被设计出来。长时程增强的形成。15-20分钟后加入强直性刺激对照刺激，诱导长时程增强。常可观察到对照组诱发电位逐渐升高的现象。为此，有必要进一步检测切片的孵化条件(温度和时间)和刺激条件(脉冲强度和间隔)。小鼠/大鼠脑片制备及膜片钳、高速钙离子成像同步测量。背部猜测目前全细胞贴片尚待最后将突触传递LTP麻醉后增加突触前，并进行高速钙离子成像。

MED64SYSTEM (Matsushita Electric Industrial) was used in respect of 64 channel multipoint extacellular stimulation/record in mouse and rat brain slice. By having constant temperature layer for noise counterplan and thermal control, the experiment was carried out in respect of the whole chamber.1. Mouse/rat brain slide preparation and multipoint recording of evoked potential.The slice of thickness 350 microns was made under the ether anesthesia in respect of the mouse in decapitation and after the cooling. As a problem in the preceding fiscal year, though the peeling in the slice piece under reflux is considerably improved, it comes in, and it is yet insufficient, and will continue in future too and will be devised.2. The formation of the long-term potentiation.15-20 By adding tetanic stimulation after the control stimulation in the minute, the long-term potentiation was induced. The phenomenon in which evoked potential of the control gradually increased was often observed. For this, it is necessary to examine incubation condition (temperature and time), and stimulation condition (intensity and interval of the pulse) of the slice further.3. Mouse/rat brain slice preparation and patch clamp, high-speed calcium imaging synchronization measurement.The back is guessed at present whole cell patch yet at last would synaptic transmission LTP anesthesia post-increase presynapse, and the high-speed calcium imaging will be carried out.