Investigation on CDDP resistance of Head and Neck Cancer.

头颈癌CDDP耐药性调查。

• 批准号: 11470352
• 负责人: INUYAMA Yukio
• 金额: $ 4.74万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11470352/
• 关键词: cis-Diamminedichloroplatinum (II); Head and Neck Cancer; Drug resistance; 頭頸部癌

a) Investigation on CDDP resistance-related genes by differential displayWe compared gene expression in the HNSCCs sensitive to CDDP with that in the resistant variants using a fluorescent differential display technique. We found that hCGα and human mt COII are highly expressed in the CDDP resistant variants.b) Investigation on CDDP resistance-related genes by cDNA microarrayWe compared gene expression in the HNSCCs sensitive to CDDP with that in the resistant variants using the cDNA microarray method. We found that five genes are differentially expressed between CDDP variants and the parental cells.c) CDDP resistance-related genes in clinical samplesWe compared gene expression in the biopsy materials from two cases of maxillary cancer using the cDNA microarray method. By using T7-based RNA amplification, we were able to analyze the difference in the gene expression of the small biopsy specimens.

a）差异显示法研究顺铂耐药相关基因我们采用荧光差异显示技术比较了顺铂敏感的HNSCC与耐药变异体的基因表达。B）CDDP耐药相关基因的cDNA微阵列研究应用cDNA微阵列技术比较了CDDP敏感和耐药细胞株中基因表达的差异。我们发现5个基因在CDDP变异体和亲本细胞之间差异表达。c）临床样本中CDDP耐药相关基因我们使用cDNA微阵列方法比较了两例上颌癌活检材料中的基因表达。通过使用基于T7的RNA扩增，我们能够分析小活检标本的基因表达差异。

项目成果

樋口 栄作: "coop耐性ヒト頭頸部扁平上皮癌細胞株におけるhcGeおよびhuman mtcoII遺伝子発理の増強"北海道医誌. 74巻3号. 231-238 (1999)

Eisaku Higuchi：“coop 抗性人头颈鳞状细胞癌细胞系中 hcGe 和人 mtcoII 基因表达的增强”北海道医学杂志，第 74 卷，第 3 期。231-238 (1999)

Nagahashi T.: "Concurrent chemoradiotherapy in hypopharyngeal cancer."Head and Neck Jpn. 26. 476-482 (2000)

Nagahashi T.：“下咽癌的同步放化疗。”Head and Neck Jpn。

Ito D: "Induction of CTL responses by simultaneousadministration of liposomal peptide vaccine with anti-CD40 and anti-CTLA-4 mAb."Journal of Immunology. 164. 1230-1235 (2000)

Ito D：“通过同时施用脂质体肽疫苗与抗 CD40 和抗 CTLA-4 mAb 来诱导 CTL 反应。”免疫学杂志。

Homma A.: "Correlation of clinicopathological parameters and biological markers related to apoptosis and ploliferative activity with a clinical outcome in squamous cell carcinoma of the larynx."Auris Nausus Larynx. (in print).

Homma A.：“与细胞凋亡和增殖活性相关的临床病理学参数和生物标志物与喉鳞状细胞癌临床结果的相关性。”Auris Nausus Larynx。

犬山 征夫: "頭頸部癌"癌の臨床. 45巻8号. 861-867 (1999)

Yukio Inuyama：“头颈癌”临床癌症，第 45 卷，第 861-867 期（1999 年）