Investigation on CDDP resistance of Head and Neck Cancer.

点击翻译按钮获取中文摘要

a) Investigation on CDDP resistance-related genes by differential displayWe compared gene expression in the HNSCCs sensitive to CDDP with that in the resistant variants using a fluorescent differential display technique. We found that hCGα and human mt COII are highly expressed in the CDDP resistant variants.b) Investigation on CDDP resistance-related genes by cDNA microarrayWe compared gene expression in the HNSCCs sensitive to CDDP with that in the resistant variants using the cDNA microarray method. We found that five genes are differentially expressed between CDDP variants and the parental cells.c) CDDP resistance-related genes in clinical samplesWe compared gene expression in the biopsy materials from two cases of maxillary cancer using the cDNA microarray method. By using T7-based RNA amplification, we were able to analyze the difference in the gene expression of the small biopsy specimens.