Study on matrix metalloproteinase activities and cartilage degradation of the temporomandibular joint

颞下颌关节基质金属蛋白酶活性与软骨降解的研究

基本信息

  • 批准号:
    11470400
  • 负责人:
  • 金额:
    $ 5.95万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    1999
  • 资助国家:
    日本
  • 起止时间:
    1999 至 2001
  • 项目状态:
    已结题

项目摘要

We have shown that several types of matrix metalloproteinases MMPs with different substrate specificities were detectable in synovial fluid(SF) from patients who suffered from temporomandibular disorders(TMD). Among these MMPs, existence of MMP-1,2,3, and 9 were correlated to bone morphologic changes of the TMJ. Our deta have also shown that induction of the active-form of MMP9 and MMP3 was important for cartilage degradation in TMJ with internal derangement(ID), which further proceeds to osteoarthritis. To further test the hypothesis that TMJ chondrocytes and disc cells synthesize specific MMPs in vitro upon cytokine stimulation, we isolated these cells from IL-1-injected rat TMJs. MMP9 and MMP3 syntheses were inducible in these cells by IL-1, whereas MMP2 was constitutively expressed on both cell types. Our studies also demonstrated that pro-forms of MMP9 and MMP3 were converted into their active-forms in vitro by IL-1 stimulation, whereas IL-1 had no remarkable effect on expression of inhibitors of MMPs(TIMPs). These results indicate that MMP9 and MMP3 are predominantly produced by disc cells and chondrocytes, and the activation of the MMPs may be regulated by proinflammatory cytokines(IL-1 and/or TNFα). These findings also suggest that IL-1 or TNFα, which usually exists in SF of patients with ID or OA, may have important roles in synthesis and activation of ECM-degrading proteinases, thereby exacerbating cartilage breakdown.
我们已经表明,几种类型的基质金属蛋白酶MMPs具有不同的底物特异性检测滑液(SF)从颞下颌关节紊乱病(TMD)患者谁遭受。其中MMP-1、2、3、9的表达与颞下颌关节骨形态学改变相关。我们的研究还表明,MMP 9和MMP 3的活性形式的诱导对于TMJ内紊乱(ID)的软骨降解是重要的,其进一步进展为骨关节炎。为了进一步验证TMJ软骨细胞和椎间盘细胞在体外细胞因子刺激下合成特异性MMPs的假设,我们从IL-1注射的大鼠TMJ中分离出这些细胞。MMP 9和MMP 3的合成诱导在这些细胞中的IL-1,而MMP 2组成型表达的两种细胞类型。我们的研究还表明,MMP 9和MMP 3的前体形式在体外被IL-1刺激转化为它们的活性形式,而IL-1对MMPs的抑制剂(TIMPs)的表达没有显著影响。这些结果表明,MMP 9和MMP 3主要由椎间盘细胞和软骨细胞产生,MMP的活化可能受促炎细胞因子(IL-1和/或TNFα)的调节。这些发现还表明,IL-1或TNFα,通常存在于SF ID或OA患者,可能在ECM降解蛋白酶的合成和激活中起重要作用,从而加剧软骨破坏。

项目成果

期刊论文数量(59)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Ijima Y,: "Role of Interleukin-1 in Induction of Matrix Metalloproteinases Synthesized by Rat Temporomandibular Joint Chondrocytes and Disc Cells."European Journal of Oral Science. (in press). (2000)
Ijima Y,:“白细胞介素-1 在诱导大鼠颞下颌关节软骨细胞和椎间盘细胞合成的基质金属蛋白酶中的作用。”欧洲口腔科学杂志。
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    0
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Ijima Y: "Interleukin-1 Induces Production of Matrix Metalloproteiriase in Rat Temporcxriandlbuiar Joint Chondrocytes and Disc Cells"The Bulletin of Kanagawa Dental College. 27. 12-14 (1999)
Ijima Y:“白细​​胞介素-1 诱导大鼠颞关节软骨细胞和椎间盘细胞中基质金属蛋白酶的产生”神奈川牙科学院通报。
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    0
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Ijima Y, Kobayashi M, and Kubota E: "The relationship between type II procollagen-C peptide and matrix degrading factors in synovial fluid of TMDs"The Bulletin of Kanagawa Dental College. 28. 1-3 (2000)
Ijima Y、Kobayashi M、Kubota E:“TMDs 滑液中 II 型前胶原 C 肽与基质降解因子的关系”神奈川齿科大学通报。
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    0
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Ijima Y: "Role of Interleukin-1 in Induction of Matrix Metalloproteinases Synthesized by Rat Temporomandibular Joint Chondrocytes and Disc Cells"European Journal of Oral Science. 109. 50-59 (2000)
Ijima Y:“白细​​胞介素-1 在诱导大鼠颞下颌关节软骨细胞和椎间盘细胞合成的基质金属蛋白酶中的作用”欧洲口腔科学杂志。
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    0
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Kubota T: "Ideifitlfication of matrix metalloproteinase (MMPs) in synovial fluid from patients with temporomandibular disorder"European Journal of Oral Science. 106. 992-998 (1998)
Kubota T:“颞下颌疾病患者滑液中基质金属蛋白酶 (MMP) 的鉴定”欧洲口腔科学杂志。
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KUBOTA Eiro其他文献

KUBOTA Eiro的其他文献

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{{ truncateString('KUBOTA Eiro', 18)}}的其他基金

Study on a new combination chemotherapy for head and neck squamous cell carcinomas
头颈鳞癌新型联合化疗方案的研究
  • 批准号:
    21390543
  • 财政年份:
    2009
  • 资助金额:
    $ 5.95万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Study for the diagnosis of early cartilage degradation in the patient with TMJ internal derangement
颞下颌关节内紊乱患者早期软骨退化的诊断研究
  • 批准号:
    08457557
  • 财政年份:
    1997
  • 资助金额:
    $ 5.95万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Studies on Treatment Effects and Prognoses of Adoptive Immunotherapy of Cancer Patients by using autologous and allogeneic lymphocytes.
使用自体和同种异体淋巴细胞对癌症患者进行过继免疫治疗的治疗效果和预后的研究。
  • 批准号:
    06671888
  • 财政年份:
    1994
  • 资助金额:
    $ 5.95万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Studies on Adoptive Immunotherapy Utilizing Allogeneic Spleen Cells Against Cancers
利用同种异体脾细胞对抗癌症的过继免疫疗法的研究
  • 批准号:
    03670949
  • 财政年份:
    1991
  • 资助金额:
    $ 5.95万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

相似海外基金

Histological tissue response and synovial fluid analysis after arthroscopic surgery with electrocautery in TMJ
颞下颌关节镜电灼手术后的组织学组织反应和滑液分析
  • 批准号:
    12671977
  • 财政年份:
    2000
  • 资助金额:
    $ 5.95万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Clinical signs and synovial fluid analysis in the temporomandibular joint of mandibular prognathism patients
下颌前突患者颞下颌关节临床体征及滑液分析
  • 批准号:
    08672319
  • 财政年份:
    1996
  • 资助金额:
    $ 5.95万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Research on diagnosis of temporomandibular disorders using synovial fluid analysis
滑液分析诊断颞下颌关节紊乱病的研究
  • 批准号:
    07838027
  • 财政年份:
    1995
  • 资助金额:
    $ 5.95万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
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