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Histological tissue response and synovial fluid analysis after arthroscopic surgery with electrocautery in TMJ

颞下颌关节镜电灼手术后的组织学组织反应和滑液分析

基本信息

批准号：
12671977
负责人：
KAKUDO Kenji
金额：
$ 2.05万
依托单位：
OSAKA DENTAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671977/
关键词：
TMJ ARTHROSCOPY ELECTROCAUTERY WOUND HEALING SHEEP SYNOVIAL FLUID

项目摘要

Aims : To evaluate the tissue response and synovial fluid analysis after arthroscopic lysis with electrocautery in the temporomandibular joint (TMJ) of sheep histologically, radiologically and biochemicallyMethod : Thirteen adult merino sheep, weighting about 60 kg, were used. The instruments used in this study were a newly developed artroscope with a channel (1.1mm in diameter) for TMJ surgery (Int. J. Oral Maxillofac. Surg.26S : 159, 1997), Electrocautery was inserted into the inner channel and the tip placed directly against the synovial membrane of the posterior pouch and the surface was injured from medial to lateral walls by electrocautery with a power of 125 watts. The tip is then placed against the articular surface of the tuber which was damaged from medial to lateral part with electrocautery until the underlining bone was exposed. At the 2nd, 4th, 8th, 12th week after the initial operation, the sheep were sacrificed by an anesthetic overdose. The specimens were decalified and sagitally sectioned from lateral to medial for histological study. Synovial fluid was collected at same time. Casein zymography was performed to detect the presence of MMP-3 in synovial fluidResults : At the 4th week after the initial operation, all discs were perforated. At the 12th week after the initial operation, the condylar articular surface was partly denuded in some areas, and part of the surface showed fibrous repair, and the disc was displaced ot the anterior portion. These canges were radiographically and histologically similar to adovanced osteoarthritis. Pro MMP-3 was detected in all experimental groupsConclusion : It was suggested that an arthroscopic lysis with electrocautery agaist the synovial membrance of the posterior pouch was induced the osteoarthritic change of sheep TMJ

目的：评价关节镜下电灼术治疗绵羊TMJ后的组织反应和滑液分析。方法：成年美利奴羊13只，体重约60 kg。在这项研究中使用的器械是一种新开发的具有通道(直径1.1 mm)的关节镜，用于TMJ手术(Int.[医]口服液。Surg.26S：159,1997)，将电灼术插入内通道，将尖端直接靠在后袋的滑膜上，用125W的电灼术从内侧壁到外侧壁损伤表面。然后将尖端贴在结节的关节表面上，结节从内侧到外侧用电灼术损坏，直到衬里的骨暴露。于首次手术后第2、4、8、12周，麻醉剂量过大处死动物。标本从外侧向内侧矢状切开，进行组织学研究。同时采集滑液。用酪蛋白酶谱检测滑液中基质金属蛋白酶-3的表达。结果：首次手术后第4周，所有关节盘均穿孔。初次手术后12周，部分区域关节面部分剥离，部分表面呈纤维性修复，关节盘前部移位。这些肿块在放射学和组织学上与晚期骨关节炎相似。结论：关节镜下电灼松解后囊滑膜可诱导绵羊TMJ骨关节炎改变。