Study for the diagnosis of early cartilage degradation in the patient with TMJ internal derangement

颞下颌关节内紊乱患者早期软骨退化的诊断研究

• 批准号: 08457557
• 负责人: KUBOTA Eiro
• 金额: $ 4.8万
• 依托单位: Kanagawa Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457557/
• 关键词: TMJ arthrosis; synovial fluid analysis; proinflammatory cytokine; matrix metalloproteinase; cartilage degradation

In this research project, biochemical markers in the synovial fluid (SF) for detecting intra-articular inflammation and early cartilage degradation of the temporomandibular joint (TMJ) disease were examined. SF was obtained from 25 TMJs in 22 patients with internal derangement (ID) or osteoarthritis (TMJ-OA), 15 asymptomatic TMJs in 11 normal volunteers. Cytokine levels were measured by ELISA,and the proteinase activities were detected by enzymography. SF from TMJs with ID and OA showed higher (p<0.05) levels (330.1(]SY.+-。[)347.7pg/100mug of SF protein) of IL-1 beta than the asymptomatic control TMJs (76.7(]SY.+-。[)95.3pg/100mug of SF protein). SF from TMJs with OA contained significantly (p<0.05) higher levels of IL-1beta (531.8(]SY.+-。[)379.6pg/100mug of SF protein) and IL-6 (979(]SY.+-。[)552pg/100mug of SF protein) than those with ID (IL-1beta : 216.7(]SY.+-。[)280.1pg, IL-6 : 293(]SY.+-。[)434pg). Two matrix metalloproteinases (MMPs) with gelatinolytic activities at 92kDa and 72kDa were constitutively detected in both the TMJ-SF (either normal or disease). Also detected were weak bands with molecular weight of 83kDa and 66kDa. Western blot analysis delineated that these were active forms of MMP-9 (83kDa) and MMP-2 (66kDa). The bands were also detected in TMJ with OA which showed high levels of IL-1beta and IL-6.These findings suggest that concomitant increases, in the levels of cytokines (IL-1 and IL-6) and active forms of MMPs, would be potential catabolic markers for cartilage degradation of the TMJ.

在本研究项目中，检测关节液（SF）中的生化标志物，用于检测关节内炎症和颞下颌关节（TMJ）疾病的早期软骨退化。对22例关节内紊乱（ID）或骨关节炎（TMJ-OA）患者的25个TMJ和11例正常志愿者的15个无症状TMJ进行SF测定。ELISA法检测细胞因子水平，酶谱法检测蛋白酶活性。来自ID和OA的TMJ的SF显示出更高（p<0.05）的水平（330.1（± 0.05）S）。[347.7pg/100 μ g SF蛋白]比无症状对照TMJ（76.7（± 0.001）pg/100 μ g SF蛋白）IL-1 β的水平高。[）95.3pg/100 mug的SF蛋白）。来自患有OA的TMJ的SF含有显著（p<0.05）更高水平的IL-1 β（531.8（± 0.05））。[] 379.6pg/100 μ g SF蛋白）和IL-6（979（± SD）。IL-1 β：552 pg/100 μ g SF蛋白）高于ID组（IL-1 β：216.7（± SD））。[] 280.1pg，IL-6：293（± SD）。[）434pg）。两种基质金属蛋白酶（MMPs）的明胶分解活性在92 kDa和72 kDa的组成型检测TMJ-SF（无论是正常或疾病）。还检测到分子量为83 kDa和66 kDa的弱条带。Western blot分析表明，这些蛋白是MMP-9（83 kDa）和MMP-2（66 kDa）的活性形式。结果表明，IL-1、IL-6和MMP活性形式的增加可能是TMJ软骨降解的潜在标志。

项目成果

Imamura H: "IL-6 and IL-8 levels in synovial fluid correlate with osteoarthritis in the temporomandibular joint" Int J Oral Maxillofac Surg in press. :209-213 (1998)

Imamura H：“滑液中的 IL-6 和 IL-8 水平与颞下颌关节骨关节炎相关”Int J Oral Maxillofac Surg 正在出版。

今村英夫: "マイクロコンピュータ利用顎関節症評価法(IMPATH/TMJ)を用いた顎関節症患者の寄与因子の評価" 日顎誌. 9・1. 139-151 (1997)

今村秀夫：“利用基于微机的颞下颌关节紊乱评估方法（IMPATH/TMJ）评估颞下颌关节紊乱患者的影响因素”Nippon Jaw Journal 9・1（1997）。

Murakami K-I: "Intra-articular levels of prostagiandin E2, hyaluronic acid,and chondroitin-4and6sulfates in the temporomadibular joint synovial fluid ofpatientswith intemal derangement" JOral Maxillofac Surg. 56. 199-203 (1998)

Murakami K-I：“内部紊乱患者颞下颌关节滑液中前列腺素 E2、透明质酸、软骨素 4 和 6 硫酸盐的关节内水平”JOral Maxillofac Surg。

今村 英夫: "マイクロコンピュータ利用顎関節症評価法(IMPATH/TMJ)を用いた顎関節症患者の寄与因子の評価" 日顎誌. 9. 139-151 (1997)

Hideo Imamura：“使用基于微型计算机的颞下颌关节紊乱评估方法（IMPATH/TMJ）评估颞下颌关节紊乱患者的影响因素”Nippon Jaw Journal 9. 139-151（1997）。

Murakami K-I: "Intra-articular levels of prostaglandin E2,hyaluronic acid,and chondroitin-4 and 6 sulfates in the temporomandibular joint synovial fluid of patients with internal derangement" J Oral Maxillofac Surg. 56. 199-203 (1998)

Murakami K-I：“内部紊乱患者颞下颌关节滑液中前列腺素 E2、透明质酸以及硫酸软骨素 4 和 6 的关节内水平”J Oral Maxillofac Surg。