The study of the biofilm formed on saliva-caoted restorative material and secondary caries

唾液覆盖修复材料上形成的生物膜与继发龋的研究

• 批准号: 11470405
• 负责人: KONISHI Norifumi
• 金额: $ 9.28万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11470405/
• 关键词: Actinomyces viscosus; Streptococcus sanguis; Streptococcus mutans; confaocal laser scanning microscopy; adherent bacteria; immunofluoresence technique; 抗A.viscosus家兎血清; 蛍光免疫染色; 染色像; 抗S.mutans家兎血清

The purpose of this study is to investigate the biofilm formed on saliva-caoted restorative material as compared with that on human enamel using immnofluoresence technique and confocal laser scanning microscopy (CLSM). Disk specimens prepared from composite resin and human enamel were retained in oral for a period time. These specimens were stained with Propidium Iodide and using FITC-Conjugated Swine Anti-Rabbit Immunogloblins with rabbit antibodies against Actinomyces viscosus, Streptococcus sanguis and Steptococcus mutans. The ratio of the adherent specific bacteria to adherent cells was examined. Furthermore, the secondary carious dentin sections were stained immunohistologically using VECTORSTAIN ABC KIT with rabbit antibodies against A. viscosus, S. sanguis and S. mutans.The findings were as follows:The levels of adherent bacteria on composite resin were higher than that on human enamel. Immnofluoresence technique and CLSM were useful tool for the observation of dental biofilm.The ratio of adherent S. mutans and to adherent cells on composite resin was same as compared with that on human enamel. The ration of adheretit S. sanguis to adherent cells was same, but the ration of adherent A, viscosus to adherent cells was different between both materials.There were no differences in the localization of the microorganisms in secondary carious dentin between composite resin material and amalgam materials.This study revealed that there were quantitative and qualitative differences in biofilms on both materials.

本研究的目的是用免疫荧光技术和激光共聚焦扫描显微镜(CLSM)研究唾液引导的修复材料和人牙釉质上形成的生物膜。将由复合树脂和人牙釉质制成的圆盘标本在口腔中保留一段时间。这些标本用碘化丙啶染色，用FITC标记的猪抗兔免疫球蛋白与兔抗粘性放线菌、血链球菌和变形链球菌抗体反应。检测贴壁特异菌与贴壁细胞的比例。应用VECTORSTAIN ABC试剂盒，用兔抗粘附性葡萄球菌、血链球菌和变形链球菌抗体对牙本质二次龋损切片进行免疫组织化学染色，结果表明：粘附菌在复合树脂上的粘附率明显高于在人牙釉质上的粘附率。免疫荧光技术和激光共聚焦显微镜是观察牙科生物膜的有效工具，与人牙釉质相比，复合树脂表面的变形链球菌黏附率和黏附细胞比率基本相同。两种材料的粘附力与附着细胞的比例相同，但粘附力与附着细胞的比例不同。复合树脂材料与银汞合金材料在牙本质中的微生物分布差异无统计学意义。

项目成果

Tomomasa TAKATSUKA et al: "Adhesion in vivo Oral Streptococci to Porcelain, Composite Resin Cement and Human Enamel"Dental Material Journal. Vol.19 No.4. 363-372 (2000)

Tomomasa TAKATSUKA 等：“口腔链球菌对瓷器、复合树脂水泥和人牙釉质的体内粘附”牙科材料杂志。

小西法文 他: "エナメル質およびレジン表面に形成されたプラーク中のmutans streptococci比率の経時的変化"日本歯科保存学雑誌. 第43巻 春季特別号. 109 (2000)

Norifumi Konishi 等人：“牙釉质和树脂表面上形成的牙菌斑中变形链球菌的比例的时间变化”，日本保守牙科杂志，第 43 卷春季特刊（2000 年）。

Norifumi Konishi et al.: "Confocal laser scanning microscopic analysis of Actinomyces viscosus adherence to composite resin"Journal of Dental Research. 80 special issue. 619 (2001)

Norifumi Konishi 等人：“粘性放线菌粘附于复合树脂的共焦激光扫描显微分析”牙科研究杂志。

Norifumi Konishi et al: "Confocal laser scanning microscopic analysis of Actinomyces viscosus adherence to composite resin"Journal of Dental Research. 80, special issue. 619 (2001)

Norifumi Konishi 等人：“粘性放线菌粘附于复合树脂的共焦激光扫描显微分析”牙科研究杂志。

Norifumi Konishi et al.: "Confocal laser scanning microscopic analysis of early plaque formed on resin composite and human enamel"Journal of Oral Rehabilitation. (In press).

Norifumi Konishi 等人：“树脂复合材料和人牙釉质上形成的早期牙菌斑的共焦激光扫描显微分析”口腔康复杂志。