The study of the biofilm formed on saliva-caoted restorative material and secondary caries

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The purpose of this study is to investigate the biofilm formed on saliva-caoted restorative material as compared with that on human enamel using immnofluoresence technique and confocal laser scanning microscopy (CLSM). Disk specimens prepared from composite resin and human enamel were retained in oral for a period time. These specimens were stained with Propidium Iodide and using FITC-Conjugated Swine Anti-Rabbit Immunogloblins with rabbit antibodies against Actinomyces viscosus, Streptococcus sanguis and Steptococcus mutans. The ratio of the adherent specific bacteria to adherent cells was examined. Furthermore, the secondary carious dentin sections were stained immunohistologically using VECTORSTAIN ABC KIT with rabbit antibodies against A. viscosus, S. sanguis and S. mutans.The findings were as follows:The levels of adherent bacteria on composite resin were higher than that on human enamel. Immnofluoresence technique and CLSM were useful tool for the observation of dental biofilm.The ratio of adherent S. mutans and to adherent cells on composite resin was same as compared with that on human enamel. The ration of adheretit S. sanguis to adherent cells was same, but the ration of adherent A, viscosus to adherent cells was different between both materials.There were no differences in the localization of the microorganisms in secondary carious dentin between composite resin material and amalgam materials.This study revealed that there were quantitative and qualitative differences in biofilms on both materials.