Analysis and Construction of Prediction Model for Change of Microbial Community Structure in Environmental Water Based on Microbial Quinone Profile

湖泊、池塘、河流等天然水生生态系统是由多种微生物组成的。在微生物中，细菌在水生生态系统中大多数营养物质、有机物和能量通量的转化中起着至关重要的作用。异养细菌对溶解有机物的利用是水生生态系统能量流动的重要环节。生物量浓度和微生物群落结构是控制生态系统中有机物（污染物）降解能力的重要因素。生物量是一个重要的参数，因为它衡量的是生物群落在一定营养水平上储存的能量的数量。近年来，利用醌谱分析技术作为一种简单有效的混合培养微生物种群动态分析工具得到了越来越多的认可。本研究利用醌谱技术，阐明了悬浮液中微生物群落结构的变化，确定了悬浮液中微生物群落结构的日变化。通过对11个池塘、4条河流和3个潮滩的呼吸和光合醌（泛醌（UQ）、甲基萘醌（MK）、质体醌（PQ）和维生素K1 (VK1)）组成的分析，对地表水微生物群落结构进行了表征和比较。河流悬浮微生物中醌类的优势种为UQ和MK，池塘悬浮微生物和河床附着微生物中醌类的优势种为PQ-9和VK1。这说明异养细菌在河流悬浮微生物中含量丰富，但微藻和蓝藻等光合微生物在池塘悬浮细菌和河床附着微生物中占主导地位。地表水呼吸醌（Uqs和mk）浓度随BOD升高呈线性增加。通过分析这些天然水生生态系统中的醌谱，可以比较土壤和活性污泥等其他环境中的微生物群落结构。少

Natural aquatic ecosystems such as lakes, ponds, and rivers are composed of numerous kinds of microorganisms. Among the microorganisms, bacteria play a crucial role in transformations of most nutrients, organic matter and energy flux in aquatic ecosystem. Utilization of dissolved organic matter by heterotrophic bacteria constitutes a major link in the energy flow of aquatic ecosystem. Biomass concentration and microbial community structure are important factors controlling the capacity to degrade organic compounds (pollutants) in the ecosystem. Biomass is an important parameter because it measures the quantity of energy being stored at certain trophic levels within a biological community. In recent years, the technique of using quinone profiles has gained increased recognition as a simple and useful tool for the analysis of microbial population dynamics in mixed cultures.In this study, the technique of quinone profiles was applied to clarify the change in microbial community structure … More and identifying diurnal change in community structure of suspended microorganisms. The microbial community structures in surface water of 11 ponds, 4 rivers and 3 tidal flats were characterized and compared by analyzing the compositions of respiratory and photosynthetic quinones (ubiquinone (UQ), menaquinone (MK), plastoquinone (PQ) vitamin K1 (VK1 )).The dominant quinone species in river-suspended microorganisms was UQ and MK, and that in pond-suspended microorganism and riverbed-attached microorganisms was PQ-9 and VK1. This indicates that heterotrophic bacteria were abundant in the river suspended microorganisms, but photosynthetic microorganisms such as micro-algae and cyanobacteria dominated the pond suspended bacteria and the riverbed attached microorganisms. Concentration of respiratory quinones (Uqs and MKs) in the surface waters increased linearly with high BOD.The comparison of microbial community structure in other environments such as soils and activated sludges are enabled by analyzing of quinone profile in these natural aquatic ecosystems. Less