Analysis and Construction of Prediction Model for Change of Microbial Community Structure in Environmental Water Based on Microbial Quinone Profile

基于微生物醌谱的环境水体微生物群落结构变化预测模型分析与构建

• 批准号: 11480149
• 负责人: GOTO Naohiro
• 金额: $ 5.76万
• 依托单位: Toyohashi University of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11480149/
• 关键词: Microbial community structure; Quinone; River; Water quality; Diurnal change; Plastoquinone; 微生物生態; 群集構造; 河川水; キノンプロファイル; 浮遊微生物; 付着微生物; バイオマーカ; 環境水; バイオマス; 微生物多様性

Natural aquatic ecosystems such as lakes, ponds, and rivers are composed of numerous kinds of microorganisms. Among the microorganisms, bacteria play a crucial role in transformations of most nutrients, organic matter and energy flux in aquatic ecosystem. Utilization of dissolved organic matter by heterotrophic bacteria constitutes a major link in the energy flow of aquatic ecosystem. Biomass concentration and microbial community structure are important factors controlling the capacity to degrade organic compounds (pollutants) in the ecosystem. Biomass is an important parameter because it measures the quantity of energy being stored at certain trophic levels within a biological community. In recent years, the technique of using quinone profiles has gained increased recognition as a simple and useful tool for the analysis of microbial population dynamics in mixed cultures.In this study, the technique of quinone profiles was applied to clarify the change in microbial community structure … More and identifying diurnal change in community structure of suspended microorganisms. The microbial community structures in surface water of 11 ponds, 4 rivers and 3 tidal flats were characterized and compared by analyzing the compositions of respiratory and photosynthetic quinones (ubiquinone (UQ), menaquinone (MK), plastoquinone (PQ) vitamin K1 (VK1 )).The dominant quinone species in river-suspended microorganisms was UQ and MK, and that in pond-suspended microorganism and riverbed-attached microorganisms was PQ-9 and VK1. This indicates that heterotrophic bacteria were abundant in the river suspended microorganisms, but photosynthetic microorganisms such as micro-algae and cyanobacteria dominated the pond suspended bacteria and the riverbed attached microorganisms. Concentration of respiratory quinones (Uqs and MKs) in the surface waters increased linearly with high BOD.The comparison of microbial community structure in other environments such as soils and activated sludges are enabled by analyzing of quinone profile in these natural aquatic ecosystems. Less

湖泊、池塘、河流等自然水生生态系统由多种微生物组成。在众多微生物中，细菌在水生生态系统中的营养物质、有机质和能量通量的转化中起着至关重要的作用。异养细菌对溶解性有机物的利用是水生态系统能量流动的重要环节。生物量浓度和微生物群落结构是控制生态系统降解有机物（污染物）能力的重要因素。生物量是一个重要的参数，因为它衡量的是生物群落中某些营养级储存的能量数量。近年来，醌类谱技术作为一种简单有效的微生物种群动态分析工具得到了越来越多的认可，本研究应用醌类谱技术来阐明微生物群落结构的变化 ...更多信息 以及确定悬浮微生物群落结构的日变化。通过对11个池塘、4条河流和3个滩涂表层水微生物呼吸醌类和光合醌类组成的分析，对表层水微生物群落结构进行了表征和比较（泛醌（UQ）、甲基萘醌（MK）、质体醌（PQ）、维生素K1（VK 1））。河流悬浮微生物中主要的醌种类为UQ和MK，池塘悬浮微生物和河床附着微生物中的优势种为PQ-9和VK 1。这说明河流悬浮微生物中异养细菌含量丰富，但池塘悬浮细菌和河床附着微生物中以微藻、蓝藻等光合微生物为主。沃茨中呼吸性醌类化合物（Uqs和MKs）的浓度随BOD的升高而线性增加。通过对这些自然水体生态系统中醌类化合物的分析，可以比较土壤和活性污泥等其他环境中微生物的群落结构。少

项目成果

Tadao Kunihiro, Byung-Ran Lim, Yukari Naito, Hong-Ying Hu, Naohiro Goto and Koichi Fujie: "Analysis of Microbial Community Change in River Using Quinone Profile"Proceedings of 1999 Annual Conference of Japan Society of Environmental Science. 92-93 (1999)

Tadao Kunihiro、Byung-Ran Lim、Yukari Naito、Hong-Ying Hu、Naohiro Goto 和 Koichi Fujie：“利用醌谱分析河流微生物群落变化”日本环境科学学会 1999 年年会论文集。

H.-Y.Hu, T.Kunihiro, N.Goto, K.Fujie: "Evaluation of the impact of domestic wastewater on the microbial community structure in a stream"The 2nd Seminar of JSPS-MOE Core University Program on Urban Environment. 45-51 (2002)

H.-Y.Hu、T.Kunihiro、N.Goto、K.Fujie：“生活废水对溪流中微生物群落结构的影响评估”JSPS-教育部城市环境核心大学项目第二期研讨会。

T.Kunihiro, H.-Y.Hu, B.-R.Lim, N.Goto, K.Fujie: "Microbial community structures of suspended and sessile microorganisms in rivers"First IWA Asia-Pacific Regional Conference,proceedings II. 917-921 (2001)

T.Kunihiro，H.-Y.Hu，B.-R.Lim，N.Goto，K.Fujie：“河流中悬浮和固着微生物的微生物群落结构”第一届IWA亚太地区会议，会议记录II。

B.-R.Lim, H.-Y.Hu, N.Goto, K.Fujie: "Analysis of relationship between water quality and microbial community structure in river"韓国大韓環境工学会秋季大会. (水質部分). 347-348 (2001)

B.-R.Lim、H.-Y.Hu、N.Goto、K.Fujie：“河流水质与微生物群落结构的关系分析”韩国环境工程学会秋季会议（水质部分。 ）347-348（2001）。

Hong-Ying Hu: "Development of a novel solid phase extraction method for the analysis of bacterial quinones in activated sludge with a higher reliability"J.Bioscience and Bioeng.. 87(3). 378-382 (1999)

胡红英：“开发一种新型固相萃取方法，用于分析活性污泥中细菌醌，具有更高的可靠性”J.Bioscience and Bioeng.. 87(3)。