Mechanism creating Biodiversity of Alpine Plants in Sakhalin

在库页岛约60个地点进行了实地调查，制作了2000张标本，收集了500份DNA样本。我们攀登并考察了契科霍瓦山、弗拉基米罗夫卡山、日丹科山和昌阿山。Adenocaulon是Sakhalin(Fukuda et al.2002)的新记录。分析了日本及其邻近地区包括Sakhalin在内的马先丹的叶绿体DNA。日本和韩国也发现了在库页岛发现的单倍型A和E，但在千岛群岛发现了另一种单倍型D(Fujii 2003)。采用聚合酶链式反应-限制性片段长度多态性方法对热带云杉叶绿体DNA进行了分析。库页岛北部的种群在遗传上与东北亚相似，南部库页岛的种群与北海道相似。库页岛和千岛群岛裸子植物的地理分布已被阐明。明确了其特征分布型，提出了S-K指数(Takahashi，2004)。库页岛和千岛群岛的16科、29属、33种的染色体数目得到了澄清(Probatova等人，2004年)。

Field research was conducted at about 60 localities in Sakhalin.2000 sheets of the herbarium specimens were made and 500 DNA samples were collected. We climbed and surveyed Mt.Chekohova, Mt.Vladimirovka, Mt.Zhidanko, and Mt.Changa. Adenocaulon was newly recorded from Sakhalin (Fukuda et al.2002)Chloroplast DNA of Pedicularis resupinata was analyzen in Japan and its neighboring regions including Sakhalin. Haplotypes A and E found in Sakhalin are also found in Japan and Korea, but another haplotype D is found in the Kuril Islands (Fujii 2003). Chloroplast DNA of Picea Jezoensis was analyzed by PCR-RFLP methods. The population of northern Sakhalin is similar to that of northeastern Asia genetically, and that of southern Sakhalin similar to that of Hokkaido. Geographical distribution of gymnosperms are clarified in Sakhalin and the Kurils. The characteristic distribution patterns were clarified and S-K index was newly proposed (Takahashi 2004). Chromosome numbers of 16 families, 29 genera and 33 species of Sakhalin and the Kurils were clarified (Probatova et al.2004).