Functional analysis of molecular complex composed by CMV-resistance protein RCY1 and other host proteins

巨细胞病毒抗性蛋白RCY1与其他宿主蛋白组成的分子复合物的功能分析

• 批准号: 19380026
• 负责人: TAKAHASHI Hideki
• 金额: $ 12.9万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19380026/
• 关键词: ウイルス抵抗性; キュウリモザイクウイルス; 病害抵抗性遺伝子; NB-LRRタンパク質; 病害抵抗性遣伝子; NB-LRRタンパク

Resistance gene to CMV(Y), RCY1, encodes CC-NBS-LRR-containing protein. In this study, we analyzed the molecular aspect of a complex composed by RCY1 and other host proteins. In conclusion, (1) RCY1-conferred resistance to CMV(Y) required three structural domains : CC, NBS and LRR in RCY1 protein, (2) HR-cell death was induced by Agrobacterium-mediated transient expression of RCY1, but co-expression of all combination of CC, NBS and LRR domain proteins in plant cell did not induce not only the cell death but also molecular interaction among them, indicating that three domains are not able to act trans-complimentarily, (3) A protein with molecular weight of 43kDa and a NTPase containing 'GHKL'ATPase motif were identified as a RCY1-interacting proteins. These finding will give a new insight for understanding the function of RCY1 protein conferring the resistance to CMV(Y).

抗CMV(Y)基因RCY1编码CC-NBS-LRR蛋白。在这项研究中，我们分析了RCY1和其他宿主蛋白组成的复合体的分子方面。综上所述，(1)RCy1对CMV(Y)的抗性需要RCy1蛋白中的CC、NBS和LRR三个结构域，(2)农杆菌介导的RCy1瞬时表达诱导HR细胞死亡，但CC、NBS和LRR结构域蛋白在植物细胞中的共同表达不仅没有诱导细胞死亡，而且它们之间的分子相互作用表明这三个结构域不能相互作用。(3)一个相对分子质量为43 kDa的蛋白和一个含有‘GHKL’ATPase基序的NTPase被鉴定为RCy1相互作用蛋白。这些发现将为理解RCY1蛋白在抗CMV(Y)中的作用提供新的视角。

项目成果

キュウリモザイクウイルス抵抗性の強度を決定する抵抗性遺伝子産物RCY1の蓄積量

决定黄瓜花叶病毒抗性强弱的抗性基因产物RCY1的累积量

• 发表时间: 2008
• 作者: 関根健太郎;ら
• 通讯作者: ら

Spermine signaling plays a significant role in the defense response of Arabidopsis thaliana to cucumber mosaic virus

• DOI: 10.1016/j.jplph.2008.08.006
• 发表时间: 2009-01-01
• 期刊: JOURNAL OF PLANT PHYSIOLOGY
• 影响因子: 4.3
• 作者: Mitsuya, Yoshiko;Takahashi, Yoshihiro;Kusano, Tomonobu
• 通讯作者: Kusano, Tomonobu

Apple latent spherical virus vectors for reliable and effective virus-induced gene silencing among a broad range of plants including tobacco, tomato

苹果潜伏球形病毒载体，可在烟草、番茄等多种植物中实现可靠、有效的病毒诱导基因沉默

• 发表时间: 2009
• 期刊: Arabidopsis Arabidopsis thaliana cucurbits, and legumes. Virology 386
• 作者: Igarashi A.;Yamagata K.;Sugai T.;Takahashi Y.;Sugawara E.;Tamura A.;Yaegashi H.;Yamagishi N.;Takahashi T.;Isogai M.;Iakahashi H.;Ybshikawa N
• 通讯作者: Ybshikawa N

Defense system induced by elicitin-like proteins of biocontrol agent Pythium oligandrum

生防剂少雄腐霉类诱导素蛋白诱导的防御系统

• 发表时间: 2010
• 作者: Takahashi;H.;Takenaka S.
• 通讯作者: Takenaka S.

High-level expression of a resistance gene, RCYI confers extreme resistance to Cucumber mosaic virus. XIII.

抗性基因 RCYI 的高水平表达赋予对黄瓜花叶病毒的极度抗性。

• 发表时间: 2007
• 作者: Sekine K.-T.;Kawakami S.;Hase S.;Shah J.;Takahashi H.
• 通讯作者: Takahashi H.