Isotation and analysis of symptom expression genes in virus-infected by transposon-tagging method.

转座子标记法分离并分析病毒感染症状表达基因。

• 批准号: 04806005
• 负责人: TAKAHASHI Hideki
• 金额: $ 1.22万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04806005/
• 关键词: Symptom expression; Cucumber mosaic virus; Gene tagging; Arabidopsis; 植物ウイルス; バーレー遺伝子

To investigate the genetic determinants of severe chlorotic spot symptoms in cucumber mosaic virus(CMV[Y])-inoculated tobacco(Nicotiana tabacum'Ky57')leaves, symptom expression was compared among tobaccos(N.tabacum'Ky57', N.tabacum'Xanthi nc', and their hybrids)inoculated with CMV(Y), CMV(O), or pseudorecombinants of both strains of CMV.Xanthi nc showed very mild chlorotic spot shmptoms in CMV(Y)-inoculated leaves. Both CMV(O)-inoculated Ky57 and Xanthi nc leaves showed no symptoms. In pseudorecombinant-inoculated Ky57 leaves, severe symptoms were determined by chimeric CMV(O)and CMV(Y)RNA3 transcripts, which contained most of the coat protein gene of CMV(Y). Genetic analysis of severe sympotom formation in CMV(Y). or pseudorecombinantinoculated tobaccos indicated that two recessive nuclearcoded host genes determined severe symptom formation in CMV(Y)-inoculated Ky57. The two recessive host genetic loci completely cosegregated with the two recessive burley loci(yb1 and yb2 genes)of Ky57, whose character is an apparent reduction in chlorophyll of the stems. When several cultivars of N.tabacum were inoculated with CMV(Y), all burley tobaccos clearly showed severe symptoms, but most other cultivars showed very mild symptoms, These results suggest that severe chlorotic spot symptom formation in CMV(Y)-inoculated Ky57 is induced by a combination of the virus coat protein gene and two recessive host genes that are closely linked to or identical to yb genes.

为了探讨接种黄瓜花叶病毒（CMV[Y]）的烟草（Nicotiana tabacum'Ky57'）叶片严重褪绿斑症状的遗传决定因素，比较了接种CMV（Y）的烟草（N.tabacum'Ky57'、N.tabacum'Xanthi nc'及其杂交种）的症状表达， CMV(O)或两种CMV.Xanthi nc菌株的假重组体在接种CMV(Y)的叶子中表现出非常轻微的褪绿斑症状。接种 CMV(O) 的 Ky57 和 Xanthi nc 叶均未表现出任何症状。在假重组接种的Ky57叶子中，嵌合的CMV(O)和CMV(Y)RNA3转录物确定了严重的症状，其中包含CMV(Y)的大部分外壳蛋白基因。 CMV(Y)严重症状形成的遗传分析。或假重组烟草表明，两个隐性核编码宿主基因决定了接种 CMV(Y) 的 Ky57 中严重症状的形成。这两个隐性宿主基因位点与Ky57的两个隐性白肋基因位点（yb1和yb2基因）完全共分离，其特征是茎部叶绿素明显减少。当几个烟草品种接种CMV(Y)时，所有白肋烟都明显表现出严重的症状，但大多数其他品种表现出非常轻微的症状。这些结果表明，接种CMV(Y)的Ky57中严重褪绿斑症状的形成是由病毒外壳蛋白基因和与yb基因紧密连锁或相同的两个隐性宿主基因的组合诱导的。

项目成果

Takahashi,H.,Goto,N.and Ehara,Y.: "Hypersensitive response in cucumber mosaic virus-infected Arabidposis thaliana" Plant Journal. (in press). (1994)

Takahashi,H.、Goto,N.和Ehara,Y.：“黄瓜花叶病毒感染的拟南芥的过敏反应”植物杂志。

Takahashi,H. and Ehara,Y.: "Severe chlorotic spot symptoms in cucumber mosaic virus strain Y-infected tobaccos are induced by a combination of the virus coat protein gane and two host recessive genes." Molecular Plant-Microbe Interactions. 6. (1993)

高桥，H.

H.Takahashi and Y.Ehara: "Severe chlorotic spot symptoms in cucumber mosaic virus strain Y-infected tobaccos are induced by a combination of the virus coat protein gene and two host recessive genes." Mol.Plant-Microbe interact.6. 182-189 (1993)

H.Takahashi 和 Y.Ehara：“黄瓜花叶病毒 Y 株感染的烟草中的严重褪绿斑病症状是由病毒外壳蛋白基因和两个宿主隐性基因的组合引起的。”

H.Takahashi and Y.Ehara: "Changes in the activity and the polypeptide composition of the oxygen-evolving complex in photosystem II of tobacco leaves infected with cucumber mosaic virus strain Y." Mol.Plant-Microbe Interact.5. 269-272 (1992)

H.Takahashi 和 Y.Ehara：“感染黄瓜花叶病毒 Y 株的烟叶光系统 II 中释氧复合物的活性和多肽组成的变化。”

H.Takahashi and Y.Ehara: "Severe chlorotic spot symptoms in cucumber mosaic virus strain Y-infected tobaccos are induced by a combination of the virus coat protein gene and two host recessive genes" Mol.Plant-Microbe Interact. 6. 182-189 (1993)

H.Takahashi 和 Y.Ehara：“黄瓜花叶病毒 Y 株感染的烟草中的严重褪绿斑病症状是由病毒外壳蛋白基因和两个宿主隐性基因的组合诱导的”Mol.Plant-Microbe Interact。