Molecular etiological study on buccal carcinogenesis in Bangladesh

分析了在孟加拉国和日本收集的健康和病理口腔组织样本中人类乳头瘤病毒（HPV）的流行情况。PCR扩增的共识引物集(1)和新的DNA阵列系统检测25个不同的HPV基因型。PCR扩增可检出1个HPV质粒拷贝。本研究使用的DNA阵列系统可检测50份HPV质粒，无需交叉杂交。28个日本样本中有2个发现了HPV，但55个正常粘膜样本和21个来自孟加拉国的癌症样本中没有发现HPV。这些结果与先前关于日本口腔癌中HPV患病率的研究结果（在25%的口腔癌样本中检测到HPV基因组；(2)）表明，HPV感染存在几何差异，HPV在孟加拉国口腔粘膜的致癌作用不显著。可能是生活方式的差异导致了HPV检出率的差异。然而，HPV传播到口腔区域的机制仍然不清楚。

The prevalence of human papillomavirus (HPV) in healthy and pathological oral tissue samples collected in Bangladesh and Japan was analyzed. PCR amplification with a consensus primer set (1) and a novel DNA array system detecting twenty-five different HPV genotypes were utilized. PCR amplification was able to detect one copy of HPV plasmid. The DNA array system used in this study could detect fifty copies of HPV plasmid without cross hybridization. HPV was found in 2 of the 28 Japanese samples but not in any of the 55 normal mucosa or 21 cancer samples from Bangladesh. These results together with results of a previous study on prevalence of HPV in Japanese oral cancers (detection of HPV genome in 25% of oral cancer samples ; (2)) suggest that a geometrical difference in HPV infection exists, and that HPV plays an insignificant role in the carcinogenesis of buccal mucosae in Bangladesh. It is possible that differences in life styles contribute to the discrepancy in HPV detection rate. However, the mechanism of HPV transmission to the oral region is still obscure.