Molecular etiological study on buccal carcinogenesis in Bangladesh

对孟加拉和日本采集的健康和病理口腔组织标本中人乳头瘤病毒(HPV)的感染情况进行了分析。用一套通用的引物(1)和一种新的检测25种不同HPV基因型的DNA阵列系统进行了PCR扩增。聚合酶链式反应扩增可检测到1个拷贝的HPV质粒。本研究所使用的DNA阵列系统无需交叉杂交即可检测到50个拷贝的HPV质粒。在28个日本样本中有2个发现了HPV，但在来自孟加拉国的55个正常粘膜或21个癌症样本中没有任何一个发现HPV。这些结果与先前关于日本口腔癌中HPV感染率的研究结果(在25%的口腔癌样本中检测到HPV基因组；(2)表明HPV感染存在几何差异，HPV在孟加拉国口腔粘膜的癌变中起着微不足道的作用。生活方式的不同可能是造成HPV检出率差异的原因之一。然而，HPV传播到口腔区域的机制仍不清楚。

The prevalence of human papillomavirus (HPV) in healthy and pathological oral tissue samples collected in Bangladesh and Japan was analyzed. PCR amplification with a consensus primer set (1) and a novel DNA array system detecting twenty-five different HPV genotypes were utilized. PCR amplification was able to detect one copy of HPV plasmid. The DNA array system used in this study could detect fifty copies of HPV plasmid without cross hybridization. HPV was found in 2 of the 28 Japanese samples but not in any of the 55 normal mucosa or 21 cancer samples from Bangladesh. These results together with results of a previous study on prevalence of HPV in Japanese oral cancers (detection of HPV genome in 25% of oral cancer samples ; (2)) suggest that a geometrical difference in HPV infection exists, and that HPV plays an insignificant role in the carcinogenesis of buccal mucosae in Bangladesh. It is possible that differences in life styles contribute to the discrepancy in HPV detection rate. However, the mechanism of HPV transmission to the oral region is still obscure.