ROS Bursts in Plant Growth and Fertility: Evolution and Diversification of the NADPH Oxidase Family
植物生长和生育力中 ROS 的爆发:NADPH 氧化酶家族的进化和多样化
基本信息
- 批准号:456649769
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:德国
- 项目类别:Research Units
- 财政年份:
- 资助国家:德国
- 起止时间:
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
Land plant evolution has shaped fundamental changes in the alternation of generations and the fertilization mode. Whereas bryophytes possess a dominant haploid generation and fertilization occurs between flagellated spermatozoids and egg cells in archegonia on gametophytes, gametophytes in angiosperms are reduced to a low number of cells. Here, fertilization has become independent of water, but requires the interaction of tip-growing pollen tubes with the embryo sac enclosed in the ovule. The local generation of reactive oxygen species (ROS) is involved in cell wall remodelling and signalling processes on both the male and female side of reproduction. In angiosperms, Respiratory Burst Oxidase Homologs (RBOHs) have been identified as the source of specific extracellular ROS generation. The first plant RBOHs appeared before the transition to land in green algae and the protein family has continuously expanded during land plant evolution, suggesting functional diversification. The precise role of RBOH isoforms and local generation of extracellular ROS for plant growth and reproduction remains unknown in an evolutionary perspective.This project will investigate (1) the ancestral biological function of RBOH plant homologs and (2) the sub- and neo-functionalisation of RBOH within model species and between evolutionary distant plant model species. We will generate null mutants for the two RBOH isoforms of the liverwort Marchantia polymorpha and the four RBOH homologs of the moss Physcomitrium patens via reverse genetics and quantify the resulting phenotypes regarding plant growth and fertility. In order to specify and quantify ROS-dependent fertility defects, we will use and further develop intracellular and extracellular dynamic sensing of local ROS generation via genetically encoded redox biosensors. In addition, we will investigate expression domains and subcellular localisation of the four P. patens isoforms via reporter constructs. Complementation studies with algal and bryophyte RBOH homologs in A. thaliana rboh mutants with reduced tip growth and fertility will allow us to conclude on functional diversification across large evolutionary distances.Thus, this project will specify the role and functional evolution of RBOHs in local ROS generation in the apoplast during sexual reproduction in different land plant lineages. This will allow a better understanding of the complex redox processes occurring during angiosperm fertilization, as well as reveal the evolutionary roots of RBOH function in plants.
陆地植物的进化在世代交替和施肥方式上产生了根本性的变化。苔藓植物具有优势的单倍体,受精发生在鞭毛精子和配子体上的卵母细胞之间,而被子植物的配子体则减少到很少的细胞数量。在这里,受精不再依赖于水,而是需要顶端生长的花粉管与封闭在胚珠内的胚囊相互作用。活性氧(ROS)的局部生成参与了雄性和雌性生殖的细胞壁重塑和信号传递过程。在被子植物中,呼吸爆发氧化酶同源物(RBOHs)已被确定为特异性细胞外ROS生成的来源。最早的植物RBOHs出现在绿藻向陆地过渡之前,在陆地植物进化过程中,蛋白质家族不断扩大,表明功能多样化。从进化的角度来看,RBOH异构体和细胞外ROS的局部产生对植物生长和繁殖的确切作用仍然未知。该项目将研究(1)RBOH植物同源物的祖先生物学功能;(2)RBOH在模式物种内和进化距离较远的植物模式物种之间的亚功能化和新功能化。我们将通过反向遗传学的方法获得多态地苔(Marchantia polymorpha)的两个RBOH同型体和苔藓(Physcomitrium patens)的四个RBOH同源体的零突变体,并量化与植物生长和繁殖有关的结果表型。为了明确和量化ROS依赖性的生育缺陷,我们将使用并进一步开发通过基因编码的氧化还原生物传感器对局部ROS生成的细胞内和细胞外动态传感。此外,我们将通过报告结构研究四种P. patens亚型的表达域和亚细胞定位。在尖端生长和繁殖能力降低的拟南芥RBOH突变体中,与藻类和苔藓植物RBOH同源物的互补研究将使我们能够在大进化距离上得出功能多样化的结论。因此,本项目将明确RBOHs在不同陆地植物系有性生殖过程中外质体局部ROS生成中的作用和功能进化。这将有助于更好地理解被子植物受精过程中发生的复杂氧化还原过程,并揭示植物RBOH功能的进化根源。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Professorin Dr. Stefanie Müller-Schüssele其他文献
Professorin Dr. Stefanie Müller-Schüssele的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Professorin Dr. Stefanie Müller-Schüssele', 18)}}的其他基金
The Significance of Organellar Membrane Contact Sites in Photosynthetic Metabolism of Plant Cells
细胞器膜接触位点在植物细胞光合代谢中的意义
- 批准号:
387162851 - 财政年份:2017
- 资助金额:
-- - 项目类别:
Research Grants
相似海外基金
Effect of nonlinear interaction between waves on abrupt bursts of emission in ultra high harmonic ion cyclotron frequency range
波间非线性相互作用对超高谐波离子回旋加速器频率范围内突然发射的影响
- 批准号:
23K03363 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Grant-in-Aid for Scientific Research (C)
Basic and applied research on extinction bursts when treating problem behavior
处理问题行为时灭绝爆发的基础和应用研究
- 批准号:
10657028 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Tidal Disruptions of stars by SMBHsr-ray bursts and gravitational waves
SMBHsr 射线爆发和引力波对恒星的潮汐扰动
- 批准号:
2886683 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Studentship
Study of TeV gamma-ray emission mechanisms of Gamma-Ray Bursts
伽马射线暴TeV伽马射线发射机制研究
- 批准号:
23K13127 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Grant-in-Aid for Early-Career Scientists
The physics of Gamma-Ray Bursts and multi-messenger sources unveiled by the SVOM and Einstein Probe satelites
SVOM 和爱因斯坦探测器卫星揭示的伽马射线爆发和多信使源的物理学
- 批准号:
2892473 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Studentship
Global dynamo mechanism in binary neutron star mergers and its application to short gamma-ray bursts
双中子星并合中的全局发电机机制及其在短伽马射线暴中的应用
- 批准号:
23H01172 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Grant-in-Aid for Scientific Research (B)
CAREER: Fast Radio Bursts Illuminating the Unseen Universe
事业:快速射电爆发照亮了看不见的宇宙
- 批准号:
2240032 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Continuing Grant
NeTS: Medium: Managing Datacenter Traffic Bursts, Fast and Slow
NeTS:中:管理数据中心突发流量、快速和慢速
- 批准号:
2313164 - 财政年份:2023
- 资助金额:
-- - 项目类别:
Standard Grant
Localised fast radio bursts as new probes of cosmology
局域快速射电暴作为宇宙学的新探测器
- 批准号:
DE220100819 - 财政年份:2022
- 资助金额:
-- - 项目类别:
Discovery Early Career Researcher Award