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Role of myosin in plant cells

肌球蛋白在植物细胞中的作用

基本信息

批准号：
14340255
负责人：
SHIMMEN Teruo
金额：
$ 10.11万
依托单位：
University of Hyogo (2004)Himeji Institute of Technology (2002-2003)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

Cytoplasmic streaming is generally observed in plant cells and involved in intracellular transport. It has been suggested that actin-myosin system is involved in generation of the motive force of cytoplasmic streaming. We had succeeded in isolating myosin molecules involved in cytoplasmic streaming in tobacco cultured cells and lily pollen tubes. In Arabidopsis thaliana, two kinds of genes supposed to code myosin molecule had been reported (myosin VIII and myosin XI). However, the function of had been remained to be elucidated. It was supposed that myosin XI is involved in cytoplasmic streaming. It has been suggested that the N terminal region has a function to slide along actin filaments and that C terminal region has a function to bind organelles. In the present study, we tried to identify the organlles carried by mysin XI.In Arabidopsis thaliana, three classes of myosin XI gene have been identified. We raised an antibody against C terminal region of MYA2 myosin. Immunocytochemical analysis revealed that this myosin is co-localized with actin filaments in epidermal cells of leaf, root hair cells and cultured cells. Distribution of this myosin in stomatal cell suggested that this myosin may associated with peroxisomes. It has been suggested that actin filament is concerned with intracellular transport of peroxisomes. However, the moter protein was remained to be identified. Therefore, we carried out an analysis using transgenic Arabidopsis plants expressing GFP in peroxisomes. Itr was found that this myosin is really associated with peroxisomes, and suggested that this myosin is responsible for transport of peroxisomes.

细胞质流在植物细胞中普遍存在，参与细胞内物质的运输。有人认为肌动蛋白-肌球蛋白系统参与了细胞质流动动力的产生。我们已经成功地分离出烟草培养细胞和百合花粉管中参与细胞质流的肌球蛋白分子。在拟南芥中，已报道了两种可能编码肌球蛋白分子的基因（肌球蛋白VIII和肌球蛋白XI）。然而，其功能仍有待阐明。据推测，肌球蛋白XI参与细胞质流动。有人认为N末端区具有沿着肌动蛋白丝滑动的功能，而C末端区具有结合细胞器的功能。在本研究中，我们试图鉴定myosin XI携带的细胞器。在拟南芥中，myosin XI基因已经被鉴定为三类。我们制备了MYA 2肌球蛋白C端抗体。免疫细胞化学分析表明，这种肌球蛋白与肌动蛋白丝共定位于叶表皮细胞、根毛细胞和培养细胞中。这种肌球蛋白在气孔细胞中的分布表明，这种肌球蛋白可能与过氧化物酶体有关。有人认为肌动蛋白丝与过氧化物酶体的细胞内转运有关。然而，马达蛋白仍有待鉴定。因此，我们使用在过氧化物酶体中表达GFP的转基因拟南芥植物进行了分析。Itr发现这种肌球蛋白确实与过氧化物酶体有关，并建议这种肌球蛋白负责过氧化物酶体的运输。