Structure and function of cytoskeleton in plant cells.

植物细胞中细胞骨架的结构和功能。

• 批准号: 06304004
• 负责人: SHIMMEN Teruo
• 金额: $ 11.71万
• 依托单位: Himeji Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06304004/
• 关键词: cytoskeleton; actin; myosin; microtubule; calcium; cytoplasmic streaming; morphogenesis; cell division; 細胞周期; 成長; ベン毛; 細胞壁

1)Cell cycle and cytoskeleton : Protein complex pufied from plasma membrane of tobacco BY-2 cell was found to have capacity to synthesize cellulose microfibril. Dynanics of kinesin-like protein was analyzed during cell cycle of tissure cultued cell. Mechanism of organization of preprophase band of microtubules was studied. 2) Cell Growth and cytoskeleton : Strong contribution of plasma membrane and cell wall to organization of cortical microtubules was found in coenocytic algae. Role of cytoskeletons in positioning of nucleus was elucidated in protonemal cells of fern. 3) Cytoplasmic streaming : Myosin was analyzed and characterized in lily pollen tubes. Close corelation between cytoplasmic free calcium and cytoplasmic streaming was found. Important role of cell wall in organization of microfilaments was found in Vallisneria. Actin binding protein regulated by ATP was purified from Physarum. Calium wave supposed to regulate cytoplasmic streaming was found in Physarum. 4) Flagellar movement : Involvement of a third factor for organization of outer arm of flagella was found. It was found that ammonium ion control nature of phototaxis im Euglena.

1)细胞周期和细胞骨架：烟草BY-2细胞质膜蛋白质复合物具有合成纤维素微纤丝的能力。分析了驱动蛋白样蛋白在组织培养细胞周期中的动态变化。对微管前期带的组织化机制进行了研究。2)细胞生长和细胞骨架：在多核藻中发现质膜和细胞壁对皮层微管的组织有很大的贡献。阐明了蕨类植物原丝体细胞骨架在细胞核定位中的作用。3)细胞质流动：百合花粉管中的肌球蛋白进行了分析和表征。细胞质游离钙与细胞质流动密切相关，细胞壁在苦草属植物微丝组织中起重要作用。从绒泡菌中纯化了ATP调控的肌动蛋白结合蛋白。在绒泡菌中发现了钙波，推测钙波可能是调节细胞质流动的一种机制。4)鞭毛运动：发现鞭毛外臂组织的第三个因素参与其中。发现铵离子控制眼虫趋光性的性质。

项目成果

Kamiya, R.: "Exploring the function of innerand outer dynein arms with Chalamydomonas" Cell Mot.Cytoskeleton. 32. 98-102 (1995)

Kamiya, R.：“利用 Chalamydomonas 探索内部和外部动力蛋白臂的功能”Cell Mot.Cytosculpture。

Kikuyama, M.et al: "In vitro demonstration of hydration-induced calcium release in Nitella flexilis" Proc.Japan Acad.Ser. B71. 179-182 (1995)

Kikuyama, M.et al：“在 Nitella flexilis 中水合诱导的钙释放的体外演示”Proc.Japan Acad.Ser。

Mizuno K.: "A Cytoskeletal 50 kDa protein in higher plants that forms intermediate-sized filaments and stabilizes microtubules." Protoplasma. 186. 99-112 (1995)

Mizuno K.：“高等植物中的一种 50 kDa 细胞骨架蛋白，可形成中等大小的丝并稳定微管。”

Shimmen, T.et al: "Studies on cessation of cytoplasmic streaming under-induced depolarization in Nitella axilliformis" Journal of Plant Research. 108. 457-4462 (1995)

Shimmen, T.等人：“关于腋形黑藻中诱导去极化的细胞质流停止的研究”植物研究杂志。

Mizuta, S.et al: "Assembly of cortical microtubules during cold treatment of the coenocytic green alga, Chaetomorpha moniligera" Planta. 196. 190-192 (1995)

Mizuta, S.et al：“共核绿藻，Chaetomorpha moniligera 冷处理过程中皮质微管的组装”Planta。