Studies on molecular organization of actin cytoskeleton in plant cells

植物细胞肌动蛋白细胞骨架的分子组织研究

• 批准号: 11304057
• 负责人: SHIMMEN Teruo
• 金额: $ 22.19万
• 依托单位: Himeji Institute of Technology.
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11304057/
• 关键词: Cytoskeleton; Actin; Myosin; Tobacco; Actin-bundling protein; Villin; Cultured cell; Root hair; リン酸化; カルシウム; カルモジュリン; BDM; 原形質流動; 花粉管

Actin cytoskeleton is involved in cytoplasmic streaming and intracellular structure. We studied the structure and function of actin cytoskeleton and related proteins of plant cells.1) Regulation of myosin activity Ca^2Previously we had isolated motile plant myosin from lily pollen tube (Yokota and Shimmen 1994). In the present study, we found that the activity of myosin is regulated by Ca^<2+>.This is the first report of Ca^<2+> regulation of plant myosin. We also found that this myosin are equipped with calmodulin as a light chain. The present results can be a molecular basis for understanding of cytoplasmic streaming by Ca^<2+>.2) Identification of two types of myosinsWe found that tobacco cultured cells have two kinds of myosins. Both myosin was regulated by Ca^<2+>. At present, we are analyzing functions of these myosins in plant cell.3) Analysis of actin-bundling protein, villinPreviously, we had isolated actin-bundling proteins. Analysis of ammo add sequence showed that these actin-bundling proteins belong to a villin family. We studied the role of villin in organization actin filaments in plant cells.

肌动蛋白骨架参与细胞质流动和胞内结构。我们研究了植物细胞肌动蛋白骨架及其相关蛋白的结构和功能。1)肌球蛋白活性的调控Ca^2以前我们从百合花粉管中分离到了运动植物肌球蛋白（Yokota and Shimmen 1994）。在本研究中，我们发现肌球蛋白的活性受Ca^<2+>的调控。这是首次报道Ca^<2+>调控植物肌球蛋白。我们还发现这种肌凝蛋白具有钙调蛋白轻链。本研究结果可为了解Ca^<2+ bbb细胞质流动的分子基础。2)两种肌球蛋白的鉴定我们发现烟草培养细胞有两种肌球蛋白。两种肌凝蛋白均受Ca^<2+>的调控。目前，我们正在分析这些肌凝蛋白在植物细胞中的功能。3)肌动蛋白捆绑蛋白（actin-bundling protein, villin）的分析以前，我们已经分离出了肌动蛋白捆绑蛋白。ammo add序列分析表明，这些肌动蛋白捆绑蛋白属于绒毛蛋白家族。我们研究了绒毛蛋白在植物细胞组织肌动蛋白丝中的作用。

项目成果

Yokota,E他4名: "Biochemical and immunocytochemical characterization of two types of myosins in cultured tobacco BY-2 cells"Plant Physiology. 121. 525-534 (1999)

Yokota、E 和其他 4 人：“培养烟草 BY-2 细胞中两种类型肌球蛋白的生化和免疫细胞化学特征”《植物生理学》121. 525-534 (1999)。

Igarashi,H.他5名: "Actin filaments purified from tobacco cultured BY-2 cells can be translocated by plant myosin"Plant and Cell Physiology. 40. 1167-1171 (1999)

Igarashi, H. 和其他 5 人：“从烟草培养的 BY-2 细胞中纯化的肌动蛋白丝可以通过植物肌球蛋白转位”《植物和细胞生理学》40. 1167-1171 (1999)。

Shimmen, T.: "Studies on electrogenesis under high K^+ concentrations in inernodal cells of Chara corallina"Journal of Plant Research. 114. 59-66 (2001)

Shimmen，T.：“珊瑚藻节内细胞高 K 浓度下的电发生研究”植物研究杂志。

Shimmen, T.: "Electrical perception of "death message" in Chara : involvement of turgor pressure"Plant & Cell Physiology. 42. 366-373 (2001)

Shimmen, T.：“Chara 中‘死亡信息’的电感知：膨胀压力的参与”植物

Yakota, E. 他3名: "Possible involvement of protein phosphorylation in the regulation of cytoplasmic organization and streaming in root hair cells as revealed by a protein phosphatase inhibitor, calyculin A"Protoplasma. 211. 29-38 (2000)

Yakota，E. 和其他 3 人：“蛋白质磷酸酶抑制剂花萼蛋白 A 揭示了蛋白质磷酸化可能参与细胞质组织和根毛细胞流动的调节”Protoplasma。