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Comparison of chloroplast, mitochondrial and multiple nuclear DNA trees in genus Pinus subgenus Stnobus

松属 Stnobus 叶绿体、线粒体和多核 DNA 树的比较

基本信息

批准号：
14340265
负责人：
WATANO Yasuyuki
金额：
$ 6.46万
依托单位：
Chiba University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14340265/
关键词：
Pinus introgessive hybridization cytoplasmic capture Molecular phylogeny mitochondrial DNA chloroplast DNA nuclear DNA cytoPlasmic capture mitochondrial DNA

项目摘要

Hybridization and introgression have been considered to be one of the main factors that lead to phylogenetic incongruence among different data sets. In the plants of Pinaceae, chloroplast and mitochondrial DNAs may have different evolutionary histories through introgression because cpDNA and mtDNA are inherited paternally and maternally, respectively. In fact, Pinus species have insufficient reproductive barriers, and many examples of introgressive hybridization have been reported. Pinus species, therefore, could be susceptible to phylogenetic bias by hybridization and introgression. In the present study, we reconstructed cpDNA tree, mtDNA tree, and four nuclear DNA trees in the genus Pinus subgenus Strobus, and compared them in order to detect cryptic and ancient introgression episodes which caused incongruence among gene trees. Four nrDNA trees were constructed based on partial sequences of GapCp, PgiC and ESTs of Pinus taeda (PtIFG_9008, PtIFG_0464). MtDNA tree was generated based on partial sequences of intron2 of nad1 and intronl of nad5 genes. CpDNA tree was based on the dataset of Wang et al. (1999) and our additional data. In nrDNA trees, species of the section Strobus were monophyletic, and the species of the other section (Parrya) were paraphyletic. In the sec. Parrya, two species of the subsection Gerardianae formed a clade, which was a sister to the sec. Strobus. CpDNA tree was consistent with the nrDNA trees in the relationship among sections and subsections. On the other hand, mtDNA tree showed three clades, and in the two of them, each clade consisted of the combined members both from sec. Strobus and from sec Parrya subsec. Gerardianae. Because phylogenetic relationships suggested by the nrDNA and cpDNA trees are congruent and also reasonable in morphological respects, the cause of incongruence could be attributed to the bias in the mtDNA tree. Possibly, mtDNA introgression had occurred anciently between species of different sections.

杂交和渐渗一直被认为是导致不同数据集之间系统发育不一致的主要因素之一。在松科植物中，由于cpDNA和mtDNA分别是父本遗传和母本遗传，叶绿体DNA和线粒体DNA可能具有不同的进化史。事实上，松树物种没有足够的生殖屏障，已经有许多导入杂交的例子被报道。因此，通过杂交和渗入，松树物种可能容易受到系统发育偏差的影响。在本研究中，我们重建了松属亚属的cpDNA树、mtDNA树和四个核DNA树，并对它们进行了比较，以发现导致基因树之间不一致的隐蔽和古老的导入事件。根据火炬松GapCp、PGIC和EST的部分序列(PtIFG_9008、PtIFG_0464)构建了4个nrDNA树。根据nad1基因内含子2和nad5基因内含子1的部分序列构建mtDNA树。CpDNA树基于Wang等人的数据集。(1999)和我们的补充数据。在nrDNA树中，球花组的物种为单系种，另一组(Parrya)的物种为单系种。在证券交易委员会。Parrya，Gerardianae亚科的两个物种组成了一个分支，它是SEC的姐妹。斯特罗伯斯。CpDNA树与nrDNA树在亚组和亚组间的关系基本一致。另一方面，线粒体DNA树显示出三个分支，在这两个分支中，每个分支都由来自SEC的组合成员组成。Strobus和Sec Parrya Subsec。杰拉迪亚科。由于nrDNA和cpDNA树的系统发育关系是一致的，在形态上也是合理的，不一致的原因可以归因于mtDNA树的偏见。线粒体DNA的导入可能在古代就发生在不同组的物种之间。