Development of a super-parallel MR microscope for construction of a 3D image database of human embryos

开发用于构建人类胚胎3D图像数据库的超并行MR显微镜

• 批准号: 14350039
• 负责人: KOSE Katsumi
• 金额: $ 8.64万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14350039/
• 关键词: MRI; NMR; MR microscope; Human embryo; embryo; 3D image; Super-parallel; super-conducting magnet; 胚子; 3D画像

Kyoto University has a large human embryo collection (〜50,000 specimens), which were collected from 1961 to 1974. Because such a collection will never be obtained again, their nondestructive 3Dmeasurements are highly desired. Kyoto and Tsukuba Universities have therefore started a project acquiring 3D MR microscopic images of about 1,200 embryos in two years to create a 3D anatomical database for human embryology. Human embryo specimens were chemically fixed in Bouin's fluid and stored in 10% formalin solution. The embryos were imaged in the formalin to avoid susceptibility effect, with a four-or eight-channel super-parallel MR microscope using a 2.34 T/40 cm superconducting magnet. All of the embryos (about 100 speciments for each stage) were measured using a T_1 weighted 3D SE sequence. The image matrix was 128 x 128 x 256 throughout the whole stages but the voxel size was varied from (40μm)^3 to (150μm)^3. Many anatomical structures were clearly visualized in the 3D images. This result demonstrates usefulness of the anatomical image database in human embryology.

京都大学有大量的人类胚胎标本（约50,000个标本），这些标本收集于1961年至1974年。因为这样的收集将永远不会再获得，他们的非破坏性三维测量是非常需要的。因此，京都大学和筑波大学启动了一个项目，在两年内获取大约1200个胚胎的3D磁共振显微图像，为人类胚胎学创建一个3D解剖数据库。人胚胎标本用化学方法固定在布因氏液中，保存在10%福尔马林溶液中。采用四通道或八通道超平行磁共振显微镜，采用2.34 T/40 cm超导磁体，在福尔马林中对胚胎进行成像，以避免磁化效应。使用T_1加权3D SE序列测量所有胚胎（每个阶段约100个标本）。整个阶段的图像矩阵为128 × 128 × 256，但体素大小从（40μm）^3到（150μm）^3不等。许多解剖结构在三维图像中清晰可见。这一结果证明了解剖图像数据库在人类胚胎学中的有用性。

项目成果

巨瀬 勝美: "NMRイメージング"共立出版. 220 (2004)

高濑克己：“NMR 成像”Kyoritsu Shuppan 220 (2004)。

MR microscopy of the a

MR 显微镜观察

• 发表时间: 2003
• 期刊: Magnetic Resonance in Medical Science 2
• 作者: H.Kumagai;Y.Asakawa;T.Iwane;K.Midorikawa;M.Obara;Yoshioka et al.
• 通讯作者: Yoshioka et al.

Matsuda et al.: "A Super Parallel MR Microscope"Magnetic Resonance in Medicine. (発表予定).

Matsuda 等人：“超级并行 MR 显微镜”医学磁共振（待出版）。

MR microscopy of the articular cartilage with a 1.0T permanent magnet portable MR system : preliminary results

使用 1.0T 永磁便携式 MR 系统对关节软骨进行 MR 显微镜检查：初步结果

• 发表时间: 2003
• 期刊: Magnetic Resonance in Medical Science 2
• 作者: Hiroshi Yoshioka;Tomoyuki Haishi;Takaaki Uematsu;Yoshimasa Mstsuda;Yuji Itai;Katsumi Kose
• 通讯作者: Katsumi Kose

Three-dimensional snow images by MR microscopy

通过 MR 显微镜拍摄的三维雪图像

• 发表时间: 2003
• 期刊: Magnetic Resonance Imaging 21
• 作者: N.Sako;S.Imoto;Y.Tanaka;T.Kurokawa;Ozeki et al.
• 通讯作者: Ozeki et al.