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Construction of an human embryo anatomical database using high resolution MR microscope

使用高分辨率磁共振显微镜构建人类胚胎解剖数据库

基本信息

批准号：
16206012
负责人：
KOSE Katsumi
金额：
$ 32.45万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16206012/
关键词：
MRI NMR MR microscope Human embryo embryo 3D image super-conducting magnet MRI 超並列

项目摘要

Our group recently reported 3D MR microscopic images of 1,204 chemically fixed human embryos selected from the Kyoto Collection of Human Embryos. The number of image matrix for these images was limited to 128×128×256 voxels primarily because of the magnetic field strength (2.34 T) used for the image acquisition. In the present study we developed a 9.4 T MR microscope with a wide dynamic range, and acquired 3D MR microscopic images of human embryos using a 256×256×512 image matrix. Carnegie Stage (CS) 16 to 22 human embryos were selected from the Kyoto Collection of Human Embryos. The specimens were stored in NMR sample tubes filled with formalin solution and used for MR microscopy measurements. The MR microscope was developed using a 9.4 T vertical wide bore superconducting magnet, a home-built gradient probe, and a MRI console. The MRI receiver system consisted of two. MRI receivers with different gain (typically 37dB (spin-echo) / 25dB (gradient-echo) difference). NMR signals were simultaneously acquired using the two receiver channels, and MRI datasets in the k-space were synthesized from those acquired in the two channels. A 3D gradient-echo (TR=100ms, TE=5ms) pulse sequence was used for the 256×256×512 voxel image acquisition. The presented results demonstrated the effectiveness of our system. In conclusion, large matrix and high resolution MR microscopic images of human embryos were acquired using a high magnetic field and a parallel receiver system and various structures of the embryos were clearly visualized.

我们的小组最近报告了从京都人类胚胎收藏中选出的 1,204 个化学固定人类胚胎的 3D MR 显微图像。这些图像的图像矩阵数量限制为 128×128×256 体素，主要是因为用于图像采集的磁场强度 (2.34 T)。在本研究中，我们开发了具有宽动态范围的 9.4 T MR 显微镜，并使用 256×256×512 图像矩阵获取人类胚胎的 3D MR 显微图像。卡内基阶段（CS）16至22个人类胚胎选自京都人类胚胎保藏中心。标本储存在装有福尔马林溶液的 NMR 样品管中，用于 MR 显微镜测量。 MR显微镜是使用9.4 T垂直宽口径超导磁体、自制梯度探头和MRI控制台开发的。 MRI 接收器系统由两个组成。具有不同增益的 MRI 接收器（通常为 37dB（自旋回波）/25dB（梯度回波）差异）。使用两个接收器通道同时采集 NMR 信号，并根据两个通道采集的数据合成 k 空间中的 MRI 数据集。 3D 梯度回波（TR=100ms，TE=5ms）脉冲序列用于 256×256×512 体素图像采集。所呈现的结果证明了我们系统的有效性。总之，使用高磁场和并行接收系统获得了人类胚胎的大矩阵和高分辨率磁共振显微图像，并且胚胎的各种结构清晰可见。