Differential spectral imaging system for biological and environmental monitoring

用于生物和环境监测的差分光谱成像系统

• 批准号: 14350220
• 负责人: OKUYAMA Masanori
• 金额: $ 9.28万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14350220/
• 关键词: Spectral image; Differential spectrum; Differential imaging; Characteristic Extraction; Modulation imaging

The wavelength-differential imaging process has been theoretically analyzed by spectral simulation using the transmission spectra of the interferometer, sensitivity spectra of RGB cells of CCD and reflection spectrum of an object. The color of each pixel on the obtained wavelength-differential image is calculated using the spectra shown above and simulated image is reconstructed. For an example of gerbera petals, the simulated image was very close to the real wavelength-differential image.Other types of modulated differential imaging system has been developed; fluorescent-and polarization-modulation imaging system. The fluorescent-modulation imaging system provided clear fluorescent images such as chlorophyll and stamp on note even in normal bright room. The polarization-modulation imaging system selectively separated a transparent image and a reflection image from the image shown on and through a glass window.The differential-spectral imaging system has been improved into a real-time system using narrow band-pass filters (interference filters) and the dual view optics by which one can observe simultaneously two images through different optical paths from an object. The new system can even take the differential-spectral images of moving objects or objects which change their spectral characteristics with time. Change of deoxidization of human blood has been monitored over time using the system. When the blood stream of a wrist is stopped by banding, the vein near the surface of the hand must be filled with deoxyhemoglobin. The system has visualized change of the spread of deoxyhemoglobin.

利用干涉仪的透射光谱、CCD的RGB单元灵敏度光谱和物体的反射光谱，通过光谱仿真对波长差分成像过程进行了理论分析。使用上面所示的光谱计算所获得的波长差分图像上的每个像素的颜色，并重建模拟图像。以非洲菊花瓣为例，模拟得到的图像与真实的波长差分图像非常接近。该荧光调制成像系统在正常光照条件下也能获得叶绿素、邮票等清晰的荧光图像。偏振调制成像系统从玻璃窗上显示的图像中选择性地分离出透明图像和反射图像，差分光谱成像系统通过窄带滤光片（干涉滤光片）和双视光学系统改进为实时成像系统，可以通过不同的光路同时观察同一物体的两个图像。新系统甚至可以拍摄运动物体或随时间改变其光谱特性的物体的差分光谱图像。利用该系统监测了人体血液还原度随时间的变化。当手腕的血流通过绑扎而停止时，靠近手表面的静脉必须充满脱氧血红蛋白。该系统实现了脱氧血红蛋白扩散变化的可视化。

项目成果

Kaoru Yamashita, et al.: "Differential Imaging Using Fluorescence-, Polarization-and Spectrum-Modulation"The Papers of Technical Meeting on Physical Sensors. PHS-02-8. 7-10 (2002)

Kaoru Yamashita 等人：“使用荧光、偏振和光谱调制的差分成像”物理传感器技术会议论文。

K.Yamashita: "Wavelength-Differential Imaging for Extraction of Characteristic Spectra"Sensors and Actuators : A.Physical. 97-98. 179-183 (2002)

K.Yamashita：“用于提取特征光谱的波长差分成像”传感器和执行器：A.物理。

K.Yamashita et al.: "Wavelength-Differential Imaging for Extraction of Characteristic Spectra"Sensors and Actuators, A. Physical.. Vol.97-98. 179-183 (2002)

K.Yamashita 等人：“用于提取特征光谱的波长差分成像”传感器和致动器，A.Physical..Vol.97-98。

山下馨, 木村貴之, 奥山雅則, 木村一貴, 濱川圭弘: "蛍光・偏光・波長変調を用いた差分撮像"電気学会フィジカルセンサ研究科資料. PHS-02-8. 7-10 (2002)

Kaoru Yamashita、Takayuki Kimura、Masanori Okuyama、Kazuki Kimura、Keihiro Hamakawa：“使用荧光、偏振和波长调制的差分成像”来自日本物理传感器研究生院电气工程师研究所的材料 PHS-02-8。 (2002)

山下馨: "蛍光・偏光・波長変調を用いた差分撮像"電気学会フィジカルセンサ研究会資料. PHS-02-8. 7-10 (2002)

Kaoru Yamashita：“使用荧光、偏振和波长调制的差分成像”日本物理传感器研究组材料的 IEE。