Bio-material pinning to the substrate and its evaluation

在这项研究中，我们旨在建立一种新的方法，将样品分子钉住在衬底表面，并利用表面增强的振动光谱检测由于单层样品而产生的微弱信号。所得结果总结如下：1。多次红外ATR（衰减全反射）测量表明，底物“聚乙烯醇（PVA）”和样品“腺苷”分子之间存在氢键。我们尝试用黄铜制成的“转移辊”将腺苷分子固定在Si衬底上形成的PVA薄膜表面上。表面增强拉曼光谱（SERS）研究表明，腺苷分子可以通过氢键从转移辊上固定到PVA表面。将分子钉在底物上的最佳条件还有待阐明。我们提出了另一种固定样品分子的方法，即“压力附着”（PA）方法。PA法是在玻璃板上蒸发厚度为5nm的银岛膜，用抹刀将样品分子的粉末分散到银岛膜上。将多余的粉末吹干后，在覆盖有分子分散银岛膜的玻璃板上施加压力。对硝基苯甲酸（PNBA）通过电离吸附在银岛膜表面，在0 ~ 0.6 MPa的施加压力下，电离后的PNBA的SERS信号强度增加，而腺苷吸附在银岛膜表面的SERS信号在施加压力时几乎没有变化。得到的结果表明，用PA方法钉住分子取决于分子与底物表面之间的亲和力。

In this study, we aimed to establish a new method for pinning of sample molecules to the substrate surface and to detect faint signals due to the mono-layer sample with surface-enhanced vibrational spectroscopies. The results obtained are summarized as follows.1.Multiple IR ATR (attenuated total reflection) measurements indicate that hydrogen bond between the substrate"poly-vinyl alcohol (PVA)"and sample"adenosine"molecules. We have attempted to pinning adenosine molecules to the PVA thin film surface formed on the Si substrate by using a brass-made"transfer roller". Surface enhanced Raman spectral (SERS) study indicates that adenosine molecules can be pinned from the transfer roller onto the PVA surface through the hydrogen bond. The optimum condition for the pinning molecules to the substrate is yet to be elucidated.2.We have proposed another method for pinning of sample molecules, namely"pressure-attachment"(PA) method. In the PA method, Ag island films (5nm in thickness) are evaporated on glass plate and powder of the sample molecule is dispersed onto the Ag island films with spatula. After air-blowing excess powder, we applied pressure on the glass plate covered the molecule-dispersed Ag island films. Para-nitro benzoic acid (PNBA) was adsorbed on the Ag islands film surface through ionization and the SERS signals of ionized PNBA increased in intensity from the applying pressure of 0 to 0.6 MPa In contrast, the SERS signals due to adenosine adsorbed on the Ag islands surface remained nearly unchanged during applying pressure. The results obtained suggest that pinning of molecules by the PA method depends upon the affinity between the molecule and the substrate surface.