Control of the Functionality by selective modification on protein surface with calixarene compounds

本文研究了以杯芳烃为催化剂的有机介质中的细胞色素C氧化聚合芳香族化合物(与酶聚合)的反应。研究了邻苯二胺的氧化聚合反应，确定了反应速率与细胞色素c浓度、氧化化合物的加入量和一种有机溶剂有关。在合适的条件下反应几个小时后，形成了几千分子量的聚合物，但没有形成更大的聚合物。第二，研究了蛋白质的传输，以连续分离一些蛋白质。在蛋白质的正向和反向提取过程中，这两种速率都取决于萃取剂浓度、料液和回收相的pH值、盐浓度和添加酒精浓度等因素。基于上述因素建立的优化体系实现了以杯[6]芳烃羧酸为载体的液膜萃取进料相中存在的细胞色素c，并连续反萃至回收相。其次，对二胺的萃取进行了研究，以阐明钙[6]芳烃羧酸对蛋白质的识别作用。首次合成了含两个氨基的二肽化合物。对该化合物进行了提取。虽然杯[6]芳烃类化合物对二肽类化合物具有较高的萃取性能，但客体化合物建议用单一分子的杯[6]芳烃类化合物进行萃取，不形成1：2的络合物，最后制备了新型主体化合物，考察了其萃取性能。该化合物在一个杯[4]芳烃分子中含有两个吡啶和两个羧基。结果表明，该催化剂具有银的选择性。还制备了含烯丙基的杯[4]芳烃羧酸，用于银的萃取。

In this year, oxidative polymerization of aromatic compounds (polyrnerizatio with enzyme) has been investigated by using cytochrome c, which is aoluble to organic media with calixarene compounds as catalyst. Oxidative polymerization of o-phenylene diamine has been investigated and the reaction rate is confirmed to be dependent on cytchrome c concentration, added amount of oxidative compound and a kind of organic solvents. After some hours of the reaction under the adequate condition, polymer with a few thousand of molecular weight was formed, but larger polymer has not been formed.Second, transport of protein has been investigated to continuously separate of some proteins. At the forward and back extraction of proteins, both rates are dependent on some factors, such as extractant concentration, pH values of feed and recovery phases, salt concentration, and added alcohol concentration. The optimal system based on the above factors realize that cytchrome c exsisting in feed phase is extracted with liquid membrane containing calix[6]arene carboxyric acid as a carrier and continuously stripped to recovery phase.Next, the extraction of diamine has been investigated for the elucidation of protein recognition with calk[6]arene carboxylic acid. The dipeptide compound with two amino groups was firstly prepared. The extraction of the compound was carried out. Although calyix[6]arene compound showed high extraction property to the dipeptide compound, the guest compound is suggested to be extracted with a single molecule of calixarene compound which means not to form 1:2 complex.Finally, the novel host compound has been prepared to investigate the extraction property. The compound possesses two pyridil and two carboxyl groups in a single molecule of calix[4]arene. It was found to show silver selectivity. Calix[4]arene carboxylic acid with allyl groups has been also prepared to investigate silver extraction.