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Control of the Functionality by selective modification on protein surface with calixarene compounds

通过杯芳烃化合物选择性修饰蛋白质表面来控制功能

基本信息

批准号：
14350419
负责人：
INOUE Katsutoshi
金额：
$ 9.28万
依托单位：
Saga University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

In this year, oxidative polymerization of aromatic compounds (polyrnerizatio with enzyme) has been investigated by using cytochrome c, which is aoluble to organic media with calixarene compounds as catalyst. Oxidative polymerization of o-phenylene diamine has been investigated and the reaction rate is confirmed to be dependent on cytchrome c concentration, added amount of oxidative compound and a kind of organic solvents. After some hours of the reaction under the adequate condition, polymer with a few thousand of molecular weight was formed, but larger polymer has not been formed.Second, transport of protein has been investigated to continuously separate of some proteins. At the forward and back extraction of proteins, both rates are dependent on some factors, such as extractant concentration, pH values of feed and recovery phases, salt concentration, and added alcohol concentration. The optimal system based on the above factors realize that cytchrome c exsisting in feed phase is extracted with liquid membrane containing calix[6]arene carboxyric acid as a carrier and continuously stripped to recovery phase.Next, the extraction of diamine has been investigated for the elucidation of protein recognition with calk[6]arene carboxylic acid. The dipeptide compound with two amino groups was firstly prepared. The extraction of the compound was carried out. Although calyix[6]arene compound showed high extraction property to the dipeptide compound, the guest compound is suggested to be extracted with a single molecule of calixarene compound which means not to form 1:2 complex.Finally, the novel host compound has been prepared to investigate the extraction property. The compound possesses two pyridil and two carboxyl groups in a single molecule of calix[4]arene. It was found to show silver selectivity. Calix[4]arene carboxylic acid with allyl groups has been also prepared to investigate silver extraction.

近年来，人们利用细胞色素c（它不溶于有机介质），在杯芳烃化合物的催化下，研究了芳香族化合物的氧化聚合（酶聚合）。研究了邻苯二胺的氧化聚合反应，确定了反应速率与细胞色素c浓度、氧化剂用量和有机溶剂种类有关。在适当的条件下反应几个小时后，形成了分子量为几千的聚合物，但没有形成更大的聚合物。第二，研究了蛋白质的传输，以连续分离一些蛋白质。在蛋白质的正向和反向提取中，两种速率都取决于一些因素，例如提取剂浓度、进料和回收相的pH值、盐浓度和添加的醇浓度。在此基础上建立的优化体系实现了以杯[6]芳烃羧酸为载体的液膜萃取液相中的细胞色素C，并将其连续反萃至回收相，进而研究了杯[6]芳烃羧酸对二胺的萃取，以阐明其对蛋白质的识别作用。首次合成了含两个氨基的二肽化合物。进行化合物的提取。虽然杯[6]芳烃对二肽类化合物表现出很好的萃取性能，但对客体化合物的萃取最好用单分子杯芳烃萃取，即不能形成1：2的络合物。该化合物在一个杯[4]芳烃分子中含有两个吡啶和两个羧基。发现其显示出银选择性。制备了含烯丙基的杯[4]芳烃羧酸，并研究了它对银的萃取作用.